Date published: 2025-9-6
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VEGF-C Antibody (E-6) - Western Blotting - Image 357422
VEGF-C 抗体 (E-6): sc-374628. SK-N-MC (A), MH-S (B) 和 F9 (C) 全细胞裂解液中 VEGF-C 表达的 Western 印迹分析.
VEGF-C 抗体 (E-6): sc-374628. SK-N-MC (A), MH-S (B) 和 F9 (C) 全细胞裂解液中 VEGF-C 表达的 Western 印迹分析.

VEGF-C 抗体 (E-6): sc-374628

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说明书
  • VEGF-C 抗体 E-6 是小鼠单克隆 IgG2a κ，VEGF-C抗体, 在29篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的VEGF-C的N-terminus附近的氨基酸103-137之间
  • VEGF-C 抗体 (E-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 precursor and mature VEGF-C
  • 抗VEGF-C抗体（E-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VEGF-C (E-6): sc-374628的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 VEGF-C 抗体 (E-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与VEGF-C 抗体 (E-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 VEGF 抗体 包括 EG-VEGF, VEGF-B, VEGF-C和VEGF-D

VEGF-C抗体（E-6）是一种IgG2a κ小鼠单克隆VEGF-C抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源VEGF-C蛋白。VEGF-C抗体（E-6）有非偶联抗VEGF-C抗体形式和多种偶联抗VEGF-C抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。血管生成的开始被认为是肿瘤发生的早期事件，可能促进肿瘤进展和转移。已描述了几种具有血管生成活性的生长因子。这些包括成纤维细胞生长因子（FGF）、血小板衍生生长因子（PDGF）和血管内皮生长因子（VEGF）。VEGF是一种二聚糖蛋白，与PDGF具有结构同源性。已描述了由选择性mRNA剪接引起的多种VEGF变体。据推测，VEGF可能在体内起肿瘤血管生成因子的作用。另外两种蛋白质，即VEGF-B和VEGF-C，与VEGF具有显著同源性。VEGF-B在心脏和骨骼肌中大量表达，经常与VEGF共表达。VEGF-C与Flt-4和Flk-1结合并特异性激活它们。编码VEGF-B和VEGF-C的基因分别定位于染色体11q13.1和4q34.3。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VEGF-C 抗体 (E-6) 参考文献:

  1. 血管内皮生长因子是人类胶质瘤体内潜在的肿瘤血管生成因子。  |  Plate, KH., et al. 1992. Nature. 359: 845-8. PMID: 1279432
  2. 胚胎血管生成和内皮细胞分化过程中血管内皮生长因子的表达。  |  Breier, G., et al. 1992. Development. 114: 521-32. PMID: 1592003
  3. 血管内皮生长因子多肽家族。  |  Ferrara, N., et al. 1991. J Cell Biochem. 47: 211-8. PMID: 1791185
  4. VEGF-C 对眼部细胞和癌细胞中 VEGF-A 的表达具有不同的调节作用；通过 RhoA 介导的途径促进血管生成。  |  Kumar, B., et al. 2011. Angiogenesis. 14: 371-80. PMID: 21698469
  5. 在增生向肿瘤过渡期间诱导血管生成。  |  Folkman, J., et al. 1989. Nature. 339: 58-61. PMID: 2469964
  6. VEGF-C是维持肠道淋巴管和脂质吸收所必需的。  |  Nurmi, H., et al. 2015. EMBO Mol Med. 7: 1418-25. PMID: 26459520
  7. 抗体介导的VEGF-C递送可有效减轻慢性皮肤炎症。  |  Schwager, S., et al. 2018. JCI Insight. 3: PMID: 30518687
  8. VEGF-C与疑似或已知冠状动脉疾病患者的死亡率。  |  Wada, H., et al. 2018. J Am Heart Assoc. 7: e010355. PMID: 30554564
  9. 在子宫内膜异位症中，与细胞外囊泡相关的VEGF-C促进淋巴管生成和免疫细胞浸润。  |  Li, WN., et al. 2020. Proc Natl Acad Sci U S A. 117: 25859-25868. PMID: 33004630
  10. 动脉输送VEGF-C可稳定动脉粥样硬化病变。  |  Silvestre-Roig, C., et al. 2021. Circ Res. 128: 284-286. PMID: 33210556
  11. VEGF-C通过促进EMT上皮乳腺癌细胞之间的相互作用来介导肿瘤生长和转移。  |  Kong, D., et al. 2021. Oncogene. 40: 964-979. PMID: 33299122
  12. 辛伐他汀通过VEGF-C/VEGFR3/PI3K-Akt途径减轻实验性脑出血后淋巴系统的损伤。  |  Liao, J., et al. 2024. Brain Res Bull. 216: 111045. PMID: 39097032

订购信息

产品名称产品编号规格价格数量收藏夹

VEGF-C 抗体 (E-6)

sc-374628
200 µg/ml
RMB2377.00

VEGF-C (E-6): m-IgG Fc BP-HRP 套装

sc-529785
200 µg Ab; 10 µg BP
RMB2662.00

VEGF-C (E-6): m-IgGκ BP-HRP 套装

sc-522795
200 µg Ab, 40 µg BP
RMB2662.00

VEGF-C (E-6): m-IgG2a BP-HRP 套装

sc-547378
200 µg Ab; 10 µg BP
RMB2662.00

VEGF-C 抗体 (E-6) AC

sc-374628 AC
500 µg/ml, 25% agarose
RMB3129.00

VEGF-C 抗体 (E-6) HRP

sc-374628 HRP
200 µg/ml
RMB2377.00

VEGF-C 抗体 (E-6) FITC

sc-374628 FITC
200 µg/ml
RMB2482.00

VEGF-C 抗体 (E-6) PE

sc-374628 PE
200 µg/ml
RMB2580.00

VEGF-C 抗体 (E-6) Alexa Fluor® 488

sc-374628 AF488
200 µg/ml
RMB2685.00

VEGF-C 抗体 (E-6) Alexa Fluor® 546

sc-374628 AF546
200 µg/ml
RMB2685.00

VEGF-C 抗体 (E-6) Alexa Fluor® 594

sc-374628 AF594
200 µg/ml
RMB2685.00

VEGF-C 抗体 (E-6) Alexa Fluor® 647

sc-374628 AF647
200 µg/ml
RMB2685.00

VEGF-C 抗体 (E-6) Alexa Fluor® 680

sc-374628 AF680
200 µg/ml
RMB2685.00

VEGF-C 抗体 (E-6) Alexa Fluor® 790

sc-374628 AF790
200 µg/ml
RMB2685.00

VEGF-C (E-6) 中和勝肽

sc-374628 P
100 µg/0.5 ml
RMB511.00

I used this antibody for Western blot (1:100) and detected specific band around 80kd, is it precursor VEGFC?

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-08-09

Does this antibody have any neutralisation or inhibitory potential in any cell-based assays?

Asked by: Steven91
Thank you for your question. No, this antibody has not been tested for any neutralization or inhibitory use, so we do not have that information. It is recommended for detection of VEGF-C via WB, IP, PAR and ELISA.
Answered by: Tech Service
Date published: 2019-01-14

PMID: # 25796380, This Manuscript has not shown VEGFC expression by western blot. Please correct me if I am wrong.

Asked by: sdas128
Thank you for your question. This journal article is listed as a product citation for our mouse monoclonal antibody, VEGF-C (E-6): sc-374628, because this specific product catalog number and clone name were referenced in the materials and methods of the article. "Immunodetection of VEGF...was done using mouse monoclonal anti-VEGF antibody (1:1000, E-6, sc-374628; Santa Cruz Biotech, Santa Cruz...".
Answered by: Technical Support
Date published: 2017-05-15

What is the recommended secondary reagent for VEGF-C Antibody (E-6): sc-374628 in Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 7
Date published: 2017-02-03
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Rated 1 out of 5 by from Not as good as expectedNo signal on WB at all at a dilution of 1 :250 on mouse lung lysates; incubated O/N at 4oC
Date published: 2021-02-18
Rated 4 out of 5 by from works well for WB and IFShows a specific signal in WB (dilution 1:200 in BSA/TBS-T) at 40kDa for HEp-2, RKO and LoVo whole cell Lysates. When used in IF, the cells (HEp-2) were fixed in 2% formaldehyde and the dilution was adjusted to 1:50 - IF signal was cytoplasmic but still slightly diffuse
Date published: 2020-01-09
Rated 4 out of 5 by from Works well on lymphatic stainingThis antibody works well on lymphatic staining. Beautiful macrophages staining.
Date published: 2019-10-09
Rated 5 out of 5 by from Excelent antibody for western blottingI obtained really strong signal by western blotting, using a 1:500 dilution of this antibody in glioblastoma cell lines
Date published: 2019-06-27
Rated 5 out of 5 by from great featuresStrip is very noticeable， quality is very good，thank you very much
Date published: 2017-09-05
Rated 1 out of 5 by from unexpected protein sizeI tested sample with the lysates from mouse thymic epithelial cell lines and got non-specific band around 20kda. Conditions of WB ; 4-20% SDS-PAGE, Wet transfer (400mA/2hr), 1:200 primary antibody in 5% TBST.
Date published: 2017-08-31
Rated 5 out of 5 by from Great IF imageWe used this antibody for IF staining and got a very clean image showing great HeLa cells
Date published: 2017-01-09
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was extremely strong detection of the ~40/80 kDa target proteins in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for three other very strongly cross-reactive proteins.
Date published: 2016-11-10
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VEGF-C 抗体 (E-6) is rated 4.0 out of 5 by 12.
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