021-6093-6350
VEGF 抗体 (F-5): sc-365578. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.
VEGF 抗体 (F-5): sc-365578
4.5(6)
寫評論提問- VEGF抗体(F-5)是小鼠单克隆IgM κ, 在18篇文献中引用，规格为200 µg/ml
- 与氨基酸序列N-terminus（h）对应的多肽序列（VEGF-A的human起源）
- 推荐用于 mouse, rat 和 human 来源的the 189, 165 and 121 amino acid splice variants of VEGF WB, IP, IF 和 ELISA检测
- 看 VEGF (C-1): sc-7269，可查看有标记的 VEGF 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
- 目前，我们还没有完成VEGF 抗体 (F-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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描述
基因信息
蛋白序列
说明书与实验方案
研究信息
関連項目
ChemCruz™ Chemical VEGF Inhibitors or VEGF Activators可用于VEGF细胞反应的功能性分析
VEGF抗体（F-5）是一种IgM κ小鼠单克隆VEGF抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的189、165和121个氨基酸剪接变体的VEGF。VEGF抗体（F-5）为非偶联的抗VEGF抗体形式。血管生成的开始被认为是肿瘤发生的早期事件，并可能促进肿瘤进展和转移。已经发现了几种具有血管生成活性的生长因子。这些包括成纤维细胞生长因子（FGFs）、血小板衍生生长因子（PDGF）和血管内皮生长因子（VEGF）。VEGF是一种与PDGF具有结构同源性的二聚体糖蛋白。已经发现了由mRNA选择性剪接产生的几种VEGF变体。据推测，VEGF在体内可能作为肿瘤血管生成因子发挥作用，因为VEGF的表达模式与胚胎血管生成中的作用一致。一些原发肿瘤中形成VEGF mRNA，体外肿瘤细胞系产生VEGF，且VEGF的有丝分裂活性似乎仅限于内皮细胞。PDGF受体家族的一个成员Flt已被确定为VEGF的高亲和力受体。
仅限研究使用。不适用于诊断和治疗用途。
| 种属 | 基因名称 | 基因ID | 染色体位置 | 亚型（mRNA）登录号 | 蛋白质登录号 | OMIM™ 号码 |
|---|---|---|---|---|---|---|
| Human | VEGFA | Entrez Gene ID: 7422 | 6p21.1 | NM_001025366, NM_001025367, NM_001025368, NM_001025369, NM_001025370, NM_001033756, NM_001171622, NM_001171623, NM_001171624, NM_001171625, NM_001171626, NM_001171627, NM_001171628, NM_001171629, NM_001171630, NM_001204384, NM_001204385, NM_001287044, NM_001317010, NM_003376 | UniProt ID: P15692 | 192240 |
| Mouse | Vegfa | Entrez Gene ID: 22339 | 17 C | NM_001025250, NM_001025257, NM_001110266, NM_001110267, NM_001110268, NM_001287056, NM_001287057, NM_001287058, NM_001317041, NM_009505 | UniProt ID: Q00731 | N/A |
全长 VEGF 蛋白序列，免疫原序列已高亮:
MNLFRFLGDLSHLLAIILLLLKIWKSRSCAGISGKSQVLFAVVFTARYLDLFTNYISLYNTCMKVVYIACSFTTVWLIYSKFKATYDGNHDTFRVEFLVVPTAILAFLVNHDFTPLEILWTFSIYLESVAILPQLFMVSKTGEAETITSHYLFALGVYRTLYLFNWIWRYHFEGFFDLIAIVAGLVQTVLYCDFFYLYITKVLKGKKLSLPA
针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz®偶联抗体，从而可以省去使用二抗。
圣克鲁斯生物技术的抗体有超过36万的研究引文。抗VEGF抗体(F-5)在各种科学出版物中有18篇引文。
针对哺乳动物靶标蛋白的一抗，如抗VEGF 抗体 (F-5)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。 许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。
VEGF 抗体 (F-5) 规格
替代产品名称: VEGF
产品克隆名称: F-5
分类: 一抗
Clonality: 单克隆抗体
抗体同型: IgM kappa light chain
氨基酸:
抗原表位靶标: VEGF-A
抗原表位种属: human
产品反应性/检测: the 189, 165 and 121 amino acid splice variants of VEGF
种属反应性/检测: mouse, rat, human
其它哺乳动物反应性: N/A
其它非哺乳动物反应性: N/A
应用: WB, IP, IF, ELISA
瓶的信息: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin
研究引文: 18篇各种科学出版物的引文
VEGF 抗体 (F-5)可选的偶联物/形式: P: Blocking Peptide
针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.
圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗VEGF抗体(F-5)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).
我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。
组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。
单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。
偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。
正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。
優選的 VEGF 抗體是 VEGF 抗體 (C-1): sc-7269.
订购信息
| 产品名称 | 产品编号 | 规格 | 价格 | 数量 | 收藏夹 | |
VEGF 抗体 (F-5) | sc-365578 | 200 µg/ml | RMB2377.00 | |||
VEGF (F-5) 中和勝肽 | sc-365578 P | 100 µg/0.5 ml | RMB511.00 |
可以申请这个货号的免费小样吗
Asked by: 木易yj
感谢您的提问。可以随一抗的订单申请不同货号的抗体小样。
Thank you for your question. You can request antibody samples with a primary antibody order.
Answered by: Technical Service SH
Date published: 2023-07-17
Can we use this (sc-365578) for IF and WB to detect VEGF-A in rat hippocampus (using either brain slices or brain tissue)? If yes, are there any published papers on this using this antibody? Thank you!
Asked by: Jenn12
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-02-08
Which specific amino acids of VEGF is this antibody raised to detect?
Asked by: Churmy
Thank you for your question. The exact peptide sequence used to develop this mouse monoclonal antibody is considered proprietary. Please contact our Technical Service Department if you would like help with sequence analysis, or if we can provide you with a 50 amino acid range within where the antigen is mapping.
Answered by: Technical Support
Date published: 2019-01-26
What application is the blocking peptide sc-365578 P appropriate for?
Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of
5 by
Dabar from
Ottimo funzionamentoOttimo anticorpo sia per western blot che per immunoprecipitazione
Date published: 2019-01-17
Rated 4 out of
5 by
Wiley from
VEGF antibody (SC-365578)I had received this free VEGF sample, and it worked good most of the times for Western blot. There were some inconsistencies in terms of the detectability of the desired band(s) although each time I blocked and incubated the antibody with blotting membranes in freshly prepared 5% milk.
Date published: 2018-01-30
Rated 5 out of
5 by
Trav11 from
Wonderful antibodyThe VEGF Antibody (F-5): sc-365578 works very well for immunofluorescence, showing staining pattern localized to the membrane.
Date published: 2017-01-19
Rated 4 out of
5 by
Kamal from
F5 clone same as other VEGF clone but givesF5 clone same as other VEGF clone but gives lot of background still need to determine the dilutions.
Date published: 2016-05-16
Rated 4 out of
5 by
FL from
Expected results by Western blot analysisExpected results by Western blot analysis of human recombinant VEGF fusion protein. - SCBT QC
Date published: 2015-04-23
Rated 5 out of
5 by
HC from
Very nice mmunofluorescence staining of methanolVery nice mmunofluorescence staining of methanol-fixed HeLa cells showing membrane localization. - SCBT QC
Date published: 2015-03-26
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VEGF 抗体 (F-5) is rated
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版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。