Date published: 2025-9-7

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VE-cadherin-2 抗体 (F-4): sc-515467

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说明书
  • VE-cadherin-2抗体(F-4)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 免疫原氨基酸序列452-593位于mouse物种的VE-cadherin-2的内部区域内
  • 抗-VE-cadherin-2 抗体 (F-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 VE-cadherin-2
  • 抗VE-cadherin-2抗体(F-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VE-cadherin-2 (F-4): sc-515467免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是VE-cadherin-2 Antibody (F-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与VE-cadherin-2 Antibody (F-4) 打包提供(请参阅下面的订购信息)。

    快捷链接

    関連項目

    VE-cadherin-2抗体(F-4)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的VE-cadherin-2。抗VE-cadherin-2抗体(F-4)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。VE-cadherin-2,也称为原钙黏蛋白12(PCDH12),是一种1184个氨基酸的单通道I型膜蛋白,主要在心脏和胎盘等血管组织中表达。VE-cadherin-2在细胞粘附中起着关键作用,而细胞粘附对于维持组织完整性和促进形态发生过程至关重要。VE-cadherin-2的细胞外结构域包含五个同源重复序列,有助于增强结合特异性,而短的细胞内结构域则与β-catenin等细胞质蛋白相互作用,从而调节钙粘蛋白的功能。VE-cadherin-2在细胞间连接处促进同型钙依赖性聚集和粘附的作用凸显了其在血管发育和组织组织中的重要性,使VE-cadherin-2成为细胞生物学和发育过程研究的宝贵目标。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    VE-cadherin-2 抗体 (F-4) 参考文献:

    1. 人 VE-Cadherin-2 的 cDNA 克隆, 染色体图谱和表达分析。  |  Ludwig, D., et al. 2000. Mamm Genome. 11: 1030-3. PMID: 11063261
    2. 原粘连蛋白 12(VE-粘连蛋白 2)在内皮细胞, 滋养层细胞和间质细胞中表达。  |  Rampon, C., et al. 2005. Exp Cell Res. 302: 48-60. PMID: 15541725
    3. 内皮粘连蛋白与肿瘤血管生成  |  Cavallaro, U., et al. 2006. Exp Cell Res. 312: 659-67. PMID: 16256984
    4. 用原粘连蛋白 12 追踪小鼠胎盘发育过程中的糖原细胞  |  Bouillot, S., et al. 2006. Placenta. 27: 882-8. PMID: 16269175
    5. 骨髓和脐带血间充质干细胞在体外的迁移受基质衍生因子-1-CXCR4和肝细胞生长因子-c-met轴的调控,并涉及基质金属蛋白酶。  |  Son, BR., et al. 2006. Stem Cells. 24: 1254-64. PMID: 16410389
    6. 血管内皮凝集素的磷酸化控制着淋巴细胞的移出。  |  Turowski, P., et al. 2008. J Cell Sci. 121: 29-37. PMID: 18096689
    7. 缺乏原粘连蛋白12会改变胎盘的形态发生和转录谱。  |  Rampon, C., et al. 2008. Physiol Genomics. 34: 193-204. PMID: 18477666
    8. 神经发育相关基因的多态性可能与精神分裂症患者大脑形态的改变有关:初步证据。  |  Gregório, SP., et al. 2009. Psychiatry Res. 165: 1-9. PMID: 19054571
    9. 鉴定位于内皮细胞胞间连接处的新型粘连蛋白(血管内皮粘连蛋白-2)。  |  Telo', P., et al. 1998. J Biol Chem. 273: 17565-72. PMID: 9651350

    订购信息

    产品名称产品编号规格价格数量收藏夹

    VE-cadherin-2 抗体 (F-4)

    sc-515467
    200 µg/ml
    RMB2377.00

    VE-cadherin-2 (F-4): m-IgG Fc BP-HRP 套装

    sc-531383
    200 µg Ab; 10 µg BP
    RMB2662.00

    VE-cadherin-2 (F-4): m-IgGκ BP-HRP 套装

    sc-525059
    200 µg Ab, 40 µg BP
    RMB2662.00

    VE-cadherin-2 (F-4): m-IgG1 BP-HRP 套装

    sc-544517
    200 µg Ab; 20 µg BP
    RMB2662.00

    VE-cadherin-2 抗体 (F-4) AC

    sc-515467 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    VE-cadherin-2 抗体 (F-4) HRP

    sc-515467 HRP
    200 µg/ml
    RMB2377.00

    VE-cadherin-2 抗体 (F-4) FITC

    sc-515467 FITC
    200 µg/ml
    RMB2482.00

    VE-cadherin-2 抗体 (F-4) PE

    sc-515467 PE
    200 µg/ml
    RMB2580.00

    VE-cadherin-2 抗体 (F-4) Alexa Fluor® 488

    sc-515467 AF488
    200 µg/ml
    RMB2685.00

    VE-cadherin-2 抗体 (F-4) Alexa Fluor® 546

    sc-515467 AF546
    200 µg/ml
    RMB2685.00

    VE-cadherin-2 抗体 (F-4) Alexa Fluor® 594

    sc-515467 AF594
    200 µg/ml
    RMB2685.00

    VE-cadherin-2 抗体 (F-4) Alexa Fluor® 647

    sc-515467 AF647
    200 µg/ml
    RMB2685.00

    VE-cadherin-2 抗体 (F-4) Alexa Fluor® 680

    sc-515467 AF680
    200 µg/ml
    RMB2685.00

    VE-cadherin-2 抗体 (F-4) Alexa Fluor® 790

    sc-515467 AF790
    200 µg/ml
    RMB2685.00

    Hola, para este anticuerpo es mejor para bloqueo leche, BSA %??, y que porcentaje de tween? y para lavar, PBS o TBS? Hello, for this antibody is better for blocking milk, BSA, %?? and what percentage of tween? and to wash, PBS or TBS?

    Asked by: MARIACONSUELO
    Thank you for your question. We recommend blocking with 5% milk or 3% BSA with 0.05% Tween-20 for Western blot. We wash with TBS but PBS would work to. If you have additional questions, please contact Technical Support at 1800-457-3801 option 2, by emailing [email protected] or by live chat on our website at www.scbt.com so we may further assist you.
    Answered by: Santa Cruz TS
    Date published: 2021-07-07

    For Western Blot, is it recommended to use denatured or non-denatured conditions with VE-cadherin-2 (F-4): sc-515467 monoclonal antibody?

    Asked by: AbPolly
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Good for Western BlotAntibody detects VE-cadherin-2 in mouse brain tissue extract by Western blot. -SCBT QC
    Date published: 2023-09-14
    Rated 4 out of 5 by from IHC-PThis antibody is not very good for IHC-P in mouse placenta.
    Date published: 2017-04-06
    Rated 5 out of 5 by from Worth itIn the process of scientific research, there is no large deviation in the reference use
    Date published: 2017-01-18
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    VE-cadherin-2 抗体 (F-4) is rated 4.7 out of 5 by 3.
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