Date published: 2025-9-8

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VCP 抗体 (F-5): sc-133211

5.0(3)
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说明书
  • VCP抗体(F-5)是小鼠单克隆IgG2b κ, 在1篇文献中引用,规格为200 µg/ml
  • 针对氨基酸687-806,在VCP的human起源的C-terminus处进行映射
  • 推荐用于 mouse, rat 和 human 来源的VCP WB, IP, IF 和 ELISA检测
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是VCP Antibody (F-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与VCP Antibody (F-5) 打包提供(请参阅下面的订购信息)。

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関連項目

含缬氨酸蛋白(VCP),也称为TERA(过渡内质网ATP酶)或p97,是AAA ATP酶家族的成员,参与多种细胞活动。 VCP是酿酒酵母Cdc48的哺乳动物同源物,酿酒酵母Cdc48是酵母细胞有丝分裂完成所必需的蛋白质。 VCP被认为参与多种膜功能和细胞周期的调节。它与泛素化的IκB-α以及26S蛋白体结合,表明VCP在蛋白体介导的IκB-α降解中可能起作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VCP 抗体 (F-5) 参考文献:

  1. 含缬氨酸蛋白的额颞叶变性:临床表现、病理特征和发病机制。  |  Weihl, CC. 2011. Curr Alzheimer Res. 8: 252-60. PMID: 21222596
  2. 含缬氨苷蛋白(VCP)的赖氨酸甲基化对小鼠的发育和存活至关重要  |  Fusser, M., et al. 2015. PLoS One. 10: e0141472. PMID: 26544960
  3. 含缬氨酸蛋白(VCP/p97)在西尼罗河病毒的复制中发挥作用。  |  Phongphaew, W., et al. 2017. Virus Res. 228: 114-123. PMID: 27914931
  4. 含缬氨酸蛋白(VCP)的杂合子突变和对 VCP 抑制剂的耐药性。  |  Bastola, P., et al. 2019. Sci Rep. 9: 11002. PMID: 31358864
  5. 利什曼原虫中的 AAA + ATPase 含缬氨酸蛋白(VCP)/p97/Cdc48 相互作用网络。  |  Aguiar, BG., et al. 2020. Sci Rep. 10: 13135. PMID: 32753747
  6. 含缬氨苷的蛋白稳定突变 p53,促进胰腺癌生长  |  Wang, J., et al. 2021. Cancer Res. 81: 4041-4053. PMID: 34099490
  7. 含缬氨酸蛋白的致病变体会诱导神经元细胞中的溶酶体损伤和自噬调节因子的转录激活。  |  Ferrari, V., et al. 2022. Neuropathol Appl Neurobiol. 48: e12818. PMID: 35501124
  8. 帕金森病中的含缬氨酸蛋白致病变体 p.R487H  |  Piat, C., et al. 2024. Clin Park Relat Disord. 10: 100236. PMID: 38283104
  9. VCP/p97 UFMylation 能稳定 BECN1 并促进自噬的启动。  |  Wang, Z., et al. 2024. Autophagy. 20: 2041-2054. PMID: 38762759
  10. 含缬氨酸蛋白(VCP/p97)在小鼠精子获能过程中的双重作用  |  Jabloñski, M., et al. 2024. Reproduction. 168: PMID: 38912971

订购信息

产品名称产品编号规格价格数量收藏夹

VCP 抗体 (F-5)

sc-133211
200 µg/ml
RMB2377.00

VCP (F-5): m-IgGκ BP-HRP 套装

sc-534600
200 µg Ab; 40 µg BP
RMB2662.00

VCP (F-5): m-IgG2b BP-HRP 套装

sc-549269
200 µg Ab; 10 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with VCP (F-5): sc-133211 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
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Rated 5 out of 5 by from strong bandsAs we know, VCP is an independent prognostic factor in gastric cancer. We choose this antibody for WB detect VCP,we tried 1:500 dilution ,it's no problem got strong bands.Maybe it canbe futher diluted at 1:1000.
Date published: 2017-09-16
Rated 5 out of 5 by from worked welThis antibody worked well for VCP analyisis, we always use this cat#, I suggest use 1:1000 dilution for Hela cell (60ug),the target band is 90kda.
Date published: 2017-09-03
Rated 5 out of 5 by from Great Western Blot data of VCP expression in nonGreat Western Blot data of VCP expression in non-transfected 293T, human VCP transfected 293T, HeLa, NIH/3T3 and PC-12 whole cell lysates. -SCBT QC
Date published: 2014-07-24
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VCP 抗体 (F-5) is rated 5.0 out of 5 by 3.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
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