Date published: 2025-9-8
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VCP Antibody (D-9) - Western Blotting - Image 356263
VCP 抗体 (D-9): sc-133212. 在 MDA-MB-231 (A), A-431 (B), 3T3-L1 (C), Sol8 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 VCP 表达的 Western 印迹分析.
VCP 抗体 (D-9): sc-133212. 在 MDA-MB-231 (A), A-431 (B), 3T3-L1 (C), Sol8 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 VCP 表达的 Western 印迹分析.

VCP 抗体 (D-9): sc-133212

4.9(8)
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说明书
  • VCP抗体(D-9)是小鼠单克隆IgG1 κ, 在4篇文献中引用，规格为200 µg/ml
  • 针对氨基酸687-806，在VCP的human起源的C-terminus处进行映射
  • 抗-VCP 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 VCP; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 抗VCP抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VCP (D-9): sc-133212的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 VCP 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与VCP 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

VCP抗体（D-9）是一种IgG1 κ小鼠单克隆VCP抗体（也称为VCP抗体或含缬氨酸蛋白抗体），它通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（IHC-P）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人源的VCP。VCP抗体（D-9）既有非偶联形式的抗VCP抗体，也有多种偶联形式的抗VCP抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。含缬氨酸蛋白（VCP），也称为TERA（代表过渡内质网ATP酶）或p97，是AAA ATP酶家族的成员，参与多种细胞活动。VCP是酿酒酵母Cdc48的哺乳动物同源物，Cdc48是酵母细胞有丝分裂完成所必需的蛋白质。人们认为VCP参与多种膜功能和细胞周期的调节。它与泛素化的IκB-α以及26S蛋白酶体相关联，表明VCP在蛋白酶体介导的IκB-α降解中可能起作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VCP 抗体 (D-9) 参考文献:

  1. 含缬氨酸蛋白（VCP/p97）的核胞浆穿梭由其 N 结构域和 C 终止子区域调控。  |  Song, C., et al. 2015. Biochim Biophys Acta. 1853: 222-32. PMID: 25447673
  2. 含缬氨酶蛋白（VCP/p97）能够通过其 ATPase 结构域展开多泛素化蛋白质。  |  Song, C., et al. 2015. Biochem Biophys Res Commun. 463: 453-7. PMID: 26043696
  3. 含缬氨酸蛋白 VCP/p97 对于利什曼原虫的胞内发育及其在热胁迫下的生存至关重要。  |  Guedes Aguiar, B., et al. 2018. Cell Microbiol. 20: e12867. PMID: 29895095
  4. 含缬氨酸蛋白与小鼠卵母细胞减数分裂停滞的维持有关†。  |  Peng, H., et al. 2019. Biol Reprod. 100: 963-970. PMID: 30476006
  5. 银屑病中的含缬氨酸蛋白：一项临床和免疫组化研究  |  El Dein Mohamed, AS., et al. 2021. Appl Immunohistochem Mol Morphol. 29: e68-e72. PMID: 33979096
  6. 黄热病病毒招募含缬氨酸蛋白-NPL4复合物，诱导应激颗粒解体，以实现高效的病毒基因组复制。  |  Arakawa, M., et al. 2022. J Biol Chem. 298: 101597. PMID: 35063505
  7. 含缬氨酸蛋白调控的内质网应激会导致 NOD2 依赖性炎症反应。  |  Ghalandary, M., et al. 2022. Sci Rep. 12: 3906. PMID: 35273242
  8. 含缬氨酸蛋白是预测急性冠状动脉综合征及其并发症的特异性生物标记物  |  Xu, C., et al. 2022. Front Cardiovasc Med. 9: 803532. PMID: 35369356
  9. 一名额颞叶痴呆症患者的含缬氨酸蛋白Asp395Gly突变：病例报告。  |  Kobayashi, R., et al. 2022. BMC Neurol. 22: 406. PMID: 36329418
  10. 含缬氨酸蛋白（VCP/p97）对小儿急性髓细胞性白血病的预后不利，并与未折叠蛋白反应蛋白 IRE1 和 GRP78 呈负相关：儿童肿瘤学组的一份报告。  |  Hoff, FW., et al. 2023. Proteomics Clin Appl. 17: e2200109. PMID: 37287368

订购信息

产品名称产品编号规格价格数量收藏夹

VCP 抗体 (D-9)

sc-133212
200 µg/ml
RMB2377.00

VCP (D-9): m-IgG Fc BP-HRP 套装

sc-528770
200 µg Ab; 10 µg BP
RMB2662.00

VCP (D-9): m-IgGκ BP-HRP 套装

sc-521276
200 µg Ab, 40 µg BP
RMB2662.00

VCP (D-9): m-IgG1 BP-HRP 套装

sc-543119
200 µg Ab; 20 µg BP
RMB2662.00

VCP 抗体 (D-9) AC

sc-133212 AC
500 µg/ml, 25% agarose
RMB3129.00

VCP 抗体 (D-9) HRP

sc-133212 HRP
200 µg/ml
RMB2377.00

VCP 抗体 (D-9) FITC

sc-133212 FITC
200 µg/ml
RMB2482.00

VCP 抗体 (D-9) PE

sc-133212 PE
200 µg/ml
RMB2580.00

VCP 抗体 (D-9) Alexa Fluor® 488

sc-133212 AF488
200 µg/ml
RMB2685.00

VCP 抗体 (D-9) Alexa Fluor® 546

sc-133212 AF546
200 µg/ml
RMB2685.00

VCP 抗体 (D-9) Alexa Fluor® 594

sc-133212 AF594
200 µg/ml
RMB2685.00

VCP 抗体 (D-9) Alexa Fluor® 647

sc-133212 AF647
200 µg/ml
RMB2685.00

VCP 抗体 (D-9) Alexa Fluor® 680

sc-133212 AF680
200 µg/ml
RMB2685.00

VCP 抗体 (D-9) Alexa Fluor® 790

sc-133212 AF790
200 µg/ml
RMB2685.00

I am using VCP (D-9): sc-133212 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_6, h_5CST
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Rated 4 out of 5 by from 效果不错我1月22日收到该产品，组织蛋白进行Wb效果不错，特异性很好，建议一抗1:200，使用脱脂牛奶封闭。
Date published: 2018-03-23
Rated 5 out of 5 by from VCP antibody (D-9)I requested for a free sample of anti-VCP antibody and I am glad to mention that the antibody really worked very good for immunoblotting. I could get clean strong signal with the antibody. I would recommend using that antibody for experiments.
Date published: 2017-04-21
Rated 5 out of 5 by from usefulI diluted the VCP antibody in 1:500 for three times , it impressed me for its effective and clear bind
Date published: 2017-03-27
Rated 5 out of 5 by from Very good antibodyVCP antibody is used to do western blot,the result is very good.
Date published: 2017-03-07
Rated 5 out of 5 by from performed wellAntibody performed well in a difficult application requiring a very clean, efficient IP: the search for a rare binding partner (CFTR) to a very abundant protein (VCP).
Date published: 2017-01-27
Rated 5 out of 5 by from Goog probeI use anti-VCP at a dilution of 1:1000/1:5000 in TBST 1hr and is enough for an excellent western signal blot.Loading 5 μg protein isenough.
Date published: 2016-11-24
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Satisfactory Western Blot data of VCP expressionSatisfactory Western Blot data of VCP expression in non-transfected 293T, human VCP transfected 293T, HeLa, NIH/3T3 and PC-12 whole cell lysates. -SCBT QC
Date published: 2013-12-19
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VCP 抗体 (D-9) is rated 4.9 out of 5 by 8.
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