Date published: 2025-9-11
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VapA Antibody (E-6) - Western Blotting - Image 314124
VapA 抗体 (E-6): sc-390576. 马尾藻 (A) 和大肠杆菌 (B) 全细胞裂解液中 VapA 表达的 Western 印迹分析. 注意 B 道与大肠杆菌缺乏反应性.
VapA Antibody (E-6) - Western Blotting - Image 314124
VapA 抗体 (E-6): sc-390576. 马尾藻 (A) 和大肠杆菌 (B) 全细胞裂解液中 VapA 表达的 Western 印迹分析. 注意 B 道与大肠杆菌缺乏反应性.
VapA Antibody (E-6) - Western Blotting - Image 394242
VapA 抗体 (E-6) Alexa Fluor® 647: sc-390576 AF647. 直接荧光西部印迹分析 R. equi 全细胞裂解液中 VapA 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.
VapA 抗体 (E-6): sc-390576. 马尾藻 (A) 和大肠杆菌 (B) 全细胞裂解液中 VapA 表达的 Western 印迹分析. 注意 B 道与大肠杆菌缺乏反应性.

VapA 抗体 (E-6): sc-390576

3.8(4)
寫評論提問

说明书
  • VapA 抗体 E-6 是小鼠单克隆 IgG2a κ，VapA抗体, 在6篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列29-189位于Rhodococcus equi (strain: 103gb-synonym: Corynebacterium equiold-name: Rhodococcus equi)物种的virulence associated protein VapA的C-terminus
  • VapA 抗体 (E-6) 推荐用于WB, IP, IF 和 ELISA，检测R. equi 来源的 virulence associated protein VapA
  • VapA 抗体 (E-6) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取VapA (E-6): sc-390576的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是VapA Antibody (E-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与VapA Antibody (E-6) 搭配使用（请参阅下面的订购信息）。

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研究信息

関連項目

附加virulence associated protein抗体，包括VAP

VapA抗体（E-6）是一种IgG2a κ小鼠单克隆VapA抗体（也称为VapA抗体），可通过WB、IP、IF和ELISA检测R. equi来源的VapA蛋白。VapA抗体（E-6）既可作为非共轭的抗VapA抗体形式，也可作为多种共轭形式的抗VapA抗体，包括琼脂糖、PE、FITC和多种Alexa Fluor®共轭物。Rhodococcus equi是一种革兰氏阳性菌，可引起马驹和免疫受损人类的脓肉芽肿性肺炎。R. equi感染是马驹在出生后六个月内死亡的主要原因。VapE（毒力相关蛋白VapE）是由R. equi毒力质粒pREAT701（p33701）编码的含有206个氨基酸的蛋白质。存在七种毒力相关蛋白：VapA、VapC、VapD、VapE、VapF、VapG和VapH。受感染的马驹通常会对R. equi感染产生免疫反应，大多数受感染的马驹表达抗VapA的抗体，而抗Vap D、F、G和H的表达水平则依次降低。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VapA 抗体 (E-6) 参考文献:

  1. 马尾罗杆菌 ATCC 33701 和 103 的 DNA 序列和毒力质粒比较。  |  Takai, S., et al. 2000. Infect Immun. 68: 6840-7. PMID: 11083803
  2. 肺炎马驹和健康暴露马驹及成年马对马霍乱球菌毒力相关蛋白的免疫球蛋白 G 亚型反应。  |  Hooper-McGrevy, KE., et al. 2003. Clin Diagn Lab Immunol. 10: 345-51. PMID: 12738629
  3. 删除编码病毒相关蛋白 A 的 vapA 可减弱细胞内放线菌马尾球菌的作用。  |  Jain, S., et al. 2003. Mol Microbiol. 50: 115-28. PMID: 14507368
  4. 马号球菌分泌的抗原具有免疫原性，可刺激对马号球菌感染免疫的马肺部产生 1 型召回反应。  |  Kohler, AK., et al. 2003. Infect Immun. 71: 6329-37. PMID: 14573652
  5. 马尾黄杆菌 ATCC 33701 的毒力基因 vapA 的表达需要 LysR 型转录调节因子 VirR。  |  Russell, DA., et al. 2004. J Bacteriol. 186: 5576-84. PMID: 15317761
  6. VapI，马尾癣菌 Vap 家族的新成员。  |  Polidori, M. and Haas, A. 2006. Antonie Van Leeuwenhoek. 90: 299-304. PMID: 16871422
  7. 通过 vap 基因家族的多重 PCR 对养马场中的马结核菌进行分子鉴定。  |  Monego, F., et al. 2009. Curr Microbiol. 58: 399-403. PMID: 19205798
  8. 开发表达 vapA 和 virR 操作子的马立克氏菌减毒活疫苗潜在菌株，并在小鼠体内进行毒力评估。  |  Whitehead, AE., et al. 2012. Vet Immunol Immunopathol. 145: 479-84. PMID: 22088674
  9. 开发酶联免疫吸附试验，用于测定马血清和初乳中的马结核菌抗体水平。  |  Witkowski, L., et al. 2012. Vet Immunol Immunopathol. 149: 280-5. PMID: 22883938

订购信息

产品名称产品编号规格价格数量收藏夹

VapA 抗体 (E-6)

sc-390576
200 µg/ml
RMB2377.00

VapA (E-6): m-IgG Fc BP-HRP 套装

sc-526175
200 µg Ab; 10 µg BP
RMB2662.00

VapA 抗体 (E-6) AC

sc-390576 AC
500 µg/ml, 25% agarose
RMB3129.00

VapA 抗体 (E-6) FITC

sc-390576 FITC
200 µg/ml
RMB2482.00

VapA 抗体 (E-6) PE

sc-390576 PE
200 µg/ml
RMB2580.00

VapA 抗体 (E-6) Alexa Fluor® 488

sc-390576 AF488
200 µg/ml
RMB2685.00

VapA 抗体 (E-6) Alexa Fluor® 546

sc-390576 AF546
200 µg/ml
RMB2685.00

VapA 抗体 (E-6) Alexa Fluor® 594

sc-390576 AF594
200 µg/ml
RMB2685.00

VapA 抗体 (E-6) Alexa Fluor® 647

sc-390576 AF647
200 µg/ml
RMB2685.00

VapA 抗体 (E-6) Alexa Fluor® 680

sc-390576 AF680
200 µg/ml
RMB2685.00

VapA 抗体 (E-6) Alexa Fluor® 790

sc-390576 AF790
200 µg/ml
RMB2685.00

VapA 抗体 (E-6) B

sc-390576 B
200 µg/ml
RMB2407.00

Hi, I want to know if you have antigen available for VapA (E-6):sc-390576 antibody? We are developing an Elisa test and we need also another antibody for sandwich assay. Can you provide those?

Asked by: TonyWah
Thank you for your question. You can find all Vap-specific antibodies by following this link: https://www.scbt.com/scbt/browse/virulence-associated-protein-Antibodies/_/N-10b7os3. We currently do not offer any antibody specific against VapA other than VapA (E-6):sc-390576.
Answered by: Tech Support Europe
Date published: 2019-05-08

Hello, you have some reference about this antibody, I mean some recent paper on which is used this anti VAPA???

Asked by: salvo_papasergi
Thank you for your question. We are not yet aware of any publications citing use of this antibody, VapA (E-6): sc-390576.
Answered by: Technical Support
Date published: 2017-09-28

What is the recommended starting dilution for Western blot for sc-390576: VapA (E-6) monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Very good blotting antibodyThis antibody is very useful in labeling VapA-expressing Rhodococcus equi in immunofluorescence microscopy without cross-reaction with non-VapA expressing R. equi. It is also very useful in Western Blotting of bacteria boiled in sample buffer to release surface-associated proteins. Minimal cross-reaction with mammalian proteins. No cross reaction with other R. equi proteins. As for 'zzz88' writing a comment in this section: He/she probed HeLa lysate with this antibody and did not see any reaction. This is not surprising, as HeLa cells do not express VapA = 'Virulence-associated protein A' from Rhodococcus equi, they might express VAPA 'vesicle-associated protein' from mammals (e.g., product sc-293273, there reacting with HeLa lysate!) and I suspect the antibody was mistakenly chosen and, hence, did correctly not react! In this case, the "one-star" rating by zzz88 were not appropriate.
Date published: 2019-01-24
Rated 4 out of 5 by from !Nice punctate staining in IF. Nice punctate staining in IF. Nice punctate staining in IF.
Date published: 2018-12-17
Rated 1 out of 5 by from Didn't work for westernI tried this antibody on Hela cells whole cell lysates and unfortunately it didn't work.
Date published: 2017-08-29
Rated 5 out of 5 by from Very good Western blot data of VapA expressionVery good Western blot data of VapA expression in Rhodococcus equi and Escherichia coli whole cell lysates. -SCBT QC
Date published: 2013-09-10
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VapA 抗体 (E-6) is rated 3.8 out of 5 by 4.
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