Date published: 2025-9-8
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V-ATPase D1 Antibody (34-Z) - Western Blotting - Image 33537
V-ATPase D1 抗体 (34-Z): sc-81887. 对 HeLa 全细胞裂解液中 V-ATPase D1 的表达进行 Western 印迹分析.
V-ATPase D1 Antibody (34-Z) - Western Blotting - Image 33537
V-ATPase D1 抗体 (34-Z): sc-81887. 对 HeLa 全细胞裂解液中 V-ATPase D1 的表达进行 Western 印迹分析.
V-ATPase D1 Antibody (34-Z) - Immunohistochemistry - Image 35919
V-ATPase D1 抗体 (34-Z): sc-81887. 福尔马林固定, 石蜡包埋的人胃组织的免疫过氧化物酶染色, 显示细胞质定位.
V-ATPase D1 抗体 (34-Z): sc-81887. 对 HeLa 全细胞裂解液中 V-ATPase D1 的表达进行 Western 印迹分析.

V-ATPase D1 抗体 (34-Z): sc-81887

4.0(5)
寫評論提問

说明书
  • V-ATPase D1抗体(34-Z)是小鼠单克隆IgG1 κ, 在7篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组V-ATPase D1
  • 推荐用于 human 来源的V-ATPase D1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 目前，我们还没有完成V-ATPase D1 抗体 (34-Z)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 V-ATPase 抗体 包括 V-ATPase A1, V-ATPase α 1, V-ATPase B1, V-ATPase B2, V-ATPase C1, V-ATPase C2, V-ATPase D, V-ATPase D1, V-ATPase D2和V-ATPase G1

液泡型H+-ATP酶（V-ATP酶）是一种多亚基酶，负责真核细胞内细胞器的酸化。V-ATP酶逆着电化学梯度泵送质子，而F-ATP酶则逆转这一过程以合成ATP。V-ATP酶复合物由负责ATP水解的外周V1结构域和负责质子转运的整合V0结构域组成。V1结构域由九个亚基（A-H）组成，而V0结构域则由五个亚基（a, d, c, c' 和 c"）构成。V-ATP酶D1（ATP酶，H+转运，溶酶体，V0亚基D1），也称为ATP6V0D1、P39、VATX、VMA6、ATP6D或VPATPD，是V0结构域的d亚基。V-ATP酶D1普遍表达，并与其他V0亚基协同作用，催化多种细胞内区室的酸化，从而合成用于液泡转运的ATP。

仅限研究使用。不适用于诊断和治疗用途。

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产品名称产品编号规格价格数量收藏夹

V-ATPase D1 抗体 (34-Z)

sc-81887
100 µg/ml
RMB2504.00

I detected only 100kDa band and weak band under 37kDa in HUVECs using this antibody. That's why I am asking about the transfected data in the data sheets.  Can this antibody detect other component of V-ATP ase?

Asked by: Maxi
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody for Western blotting.
Answered by: Tech Service
Date published: 2019-11-06

The molecular weight of V-ATPase D1 is 40kDa and the WB data of Hela in the data sheet is 40kDa. But WB of human V-ATPase D1 transfected 293T cells in the data sheet is around 100kDa. Which is the true molecular weight?

Asked by: Maxi
Thank you for your question. Thank you for bringing this discrepancy to our attention. The observed molecular weight in the transfected lysate is much higher than expected. We will be reviewing this data for accuracy and will remove or replace it if necessary.
Answered by: Technical Support
Date published: 2019-11-06

Can sc-81887: V-ATPase D1 (34-Z) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, sc-81887: V-ATPase D1 (34-Z) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good specificity observed in WB in HeLa wholeGood specificity observed in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-26
Rated 4 out of 5 by from Produced robust immunoperoxidase cytoplasmicProduced robust immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-03-31
Rated 4 out of 5 by from Published data for WB in HeLa cellsPublished data for WB in HeLa cells. -SCBT Publication Review
Date published: 2015-03-28
Rated 4 out of 5 by from Good antibody for WB in human VGood antibody for WB in human V-ATPase D1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Published data for WB in epithelial tissuesPublished data for WB in epithelial tissues from human breast cancer tissue. -SCBT Publication Review
Date published: 2015-01-12
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V-ATPase D1 抗体 (34-Z) is rated 4.0 out of 5 by 5.
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