Date published: 2025-9-7
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utrophin Antibody (8A4) - Western Blotting - Image 361554
胞浆蛋白抗体 (8A4): sc-33700. AN3CA (A) 和 NIH/3T3 (B) 全细胞裂解液中 utrophin 表达的 Western 印迹分析.
胞浆蛋白抗体 (8A4): sc-33700. AN3CA (A) 和 NIH/3T3 (B) 全细胞裂解液中 utrophin 表达的 Western 印迹分析.

utrophin 抗体 (8A4): sc-33700

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说明书
  • utrophin 抗体 8A4 是小鼠单克隆 IgG1 κ，utrophin抗体, 在28篇文献中引用，规格为200 µg/ml
  • 与human的utrophin的重组片段
  • utrophin 抗体 (8A4) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 Xenopus来源的utrophin
  • 抗utrophin抗体（8A4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取utrophin (8A4): sc-33700的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 utrophin 抗体 (8A4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与utrophin 抗体 (8A4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

utrophin抗体（8A4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的utrophin蛋白。抗utrophin 抗体（8A4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Utrophin在肌肉功能和细胞完整性方面发挥着至关重要的作用，因为utrophin是一种结构性的肌动蛋白结合蛋白，有助于将细胞骨架锚定在质膜上，与与杜兴氏和贝克尔肌肉营养不良症相关的dystrophin类似。Utrophin的重要性在于它能够弥补肌肉组织中dystrophin的缺失，特别是在肌肉营养不良症中。研究表明，上调utrophin可以减轻dystrophin缺乏症的病理影响，为治疗这些衰弱性疾病提供了潜在的治疗途径。此外，utrophin与各种蛋白质（包括与dystrophin相关的糖蛋白）的相互作用凸显了utrophin在维持肌肉纤维结构完整性和促进细胞骨架与细胞外基质之间沟通方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

utrophin 抗体 (8A4) 参考文献:

  1. 哺乳动物神经肌肉突触中utrophin表达的调节和功能意义。  |  Gramolini, AO., et al. 2000. Microsc Res Tech. 49: 90-100. PMID: 10757882
  2. 作为杜氏和贝克型肌营养不良症诊断标志物的肌营养不良蛋白。免疫荧光与 Western 印迹的相关性。  |  Voit, T., et al. 1991. Neuropediatrics. 22: 152-62. PMID: 1944822
  3. 肌营养不良蛋白是杜氏/贝克尔肌营养不良症基因的蛋白质产物。  |  Monaco, AP. 1989. Trends Biochem Sci. 14: 412-5. PMID: 2683261
  4. Utrophin影响营养不良肌肉中的线粒体病理和氧化应激。  |  Kennedy, TL., et al. 2017. Skelet Muscle. 7: 22. PMID: 29065908
  5. Utrophin调节剂药物作为杜兴氏和贝克尔肌肉萎缩症的潜在疗法。  |  Soblechero-Martín, P., et al. 2021. Neuropathol Appl Neurobiol. 47: 711-723. PMID: 33999469
  6. 通过AhR拮抗作用发现喹唑啉和喹啉类小分子作为Utrophin上调剂治疗杜兴氏肌肉萎缩症。  |  Ghosh, S., et al. 2024. J Med Chem. 67: 9260-9276. PMID: 38771158
  7. 肌动蛋白肌动蛋白结合域：肌动蛋白结合和细胞靶向分析。  |  Winder, SJ., et al. 1995. J Cell Sci. 108 (Pt 1): 63-71. PMID: 7738117
  8. 肌营养不良蛋白相关糖蛋白：它们在杜氏肌营养不良症发病机制中可能发挥的作用。  |  Ervasti, JM. and Campbell, KP. 1993. Mol Cell Biol Hum Dis Ser. 3: 139-66. PMID: 8111538
  9. 肌营养不良蛋白的糖蛋白-复合物结合位点的分子结构。三种肌营养不良蛋白相关蛋白直接与肌营养不良蛋白的羧基末端结合。  |  Suzuki, A., et al. 1994. Eur J Biochem. 220: 283-92. PMID: 8125086
  10. 肌营养不良蛋白与 F-肌动蛋白相互作用的新模型。  |  Rybakova, IN., et al. 1996. J Cell Biol. 135: 661-72. PMID: 8909541
  11. 利用截短的utrophin转基因改善mdx小鼠的肌营养不良表型。  |  Tinsley, JM., et al. 1996. Nature. 384: 349-53. PMID: 8934518
  12. 胰凝乳蛋白的肌肉和神经异构体通过转录调控机制增加了培养肌管中utrophin的表达。  |  Gramolini, AO., et al. 1998. J Biol Chem. 273: 736-43. PMID: 9422725

订购信息

产品名称产品编号规格价格数量收藏夹

utrophin 抗体 (8A4)

sc-33700
200 µg/ml
RMB2377.00

utrophin (8A4): m-IgG Fc BP-HRP 套装

sc-528487
200 µg Ab; 10 µg BP
RMB2662.00

utrophin (8A4): m-IgGκ BP-HRP 套装

sc-520878
200 µg Ab, 40 µg BP
RMB2662.00

utrophin (8A4): m-IgG1 BP-HRP 套装

sc-542963
200 µg Ab; 20 µg BP
RMB2662.00

utrophin 抗体 (8A4) AC

sc-33700 AC
500 µg/ml, 25% agarose
RMB3129.00

utrophin 抗体 (8A4) HRP

sc-33700 HRP
200 µg/ml
RMB2377.00

utrophin 抗体 (8A4) FITC

sc-33700 FITC
200 µg/ml
RMB2482.00

utrophin 抗体 (8A4) PE

sc-33700 PE
200 µg/ml
RMB2580.00

utrophin 抗体 (8A4) Alexa Fluor® 488

sc-33700 AF488
200 µg/ml
RMB2685.00

utrophin 抗体 (8A4) Alexa Fluor® 546

sc-33700 AF546
200 µg/ml
RMB2685.00

utrophin 抗体 (8A4) Alexa Fluor® 594

sc-33700 AF594
200 µg/ml
RMB2685.00

utrophin 抗体 (8A4) Alexa Fluor® 647

sc-33700 AF647
200 µg/ml
RMB2685.00

utrophin 抗体 (8A4) Alexa Fluor® 680

sc-33700 AF680
200 µg/ml
RMB2685.00

utrophin 抗体 (8A4) Alexa Fluor® 790

sc-33700 AF790
200 µg/ml
RMB2685.00

Hi, Is there any negative control cell line that we can use for an WB with this antibody? Best, Anonymous

Asked by: Anonymous
Thank you for your question. We do not have any recommended negative control cell lysates available for this antibody. Positive controls are C2C12 whole cell lysate: sc-364188, SJRH30 cell lysate: sc-2287 or Caco-2 cell lysate: sc-2262. Alternatively, negative controls can be achieved with our CRISPR- or si-/shRNA-based knockout/knockdown products. Please contact our technical service team directly for further information on available options.
Answered by: Tech Support Europe
Date published: 2025-01-13

Have you ever used this antibody sc-33700 for mouse muscle utrophin WB, is there some unspecific bands?

Asked by: Anonymous
Thank you for your question. utrophin Antibody (8A4): sc-33700 is warranted on testing mouse samples by WB. Please contact our Technical Service Department for support if you have any other questions.
Answered by: Technical Service SH
Date published: 2023-01-04

Can you tell me the condition for Western blotting(or transfer condition) of UTRN? I tried to do western blotting of Utrophin, but I couldn't detect the specific band for utrophin. The reason is that utrophin is high molecular weight protein, I guess.

Asked by: Jenny3
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody. We can provide you with some specific recommendations for Western blotting of this high molecular weight protein.
Answered by: Technical Support
Date published: 2019-08-16

Hi, I was wondering whether the isotype is really IgG1. In the DSHB the same clone (Mancho3; 8A4) is described as IgG2a. I need to know whether I can establish a co-staining using isotype specific secondary abs. Can you confirm the isotype? Many thanks.

Asked by: Sebastian M
Thank you for your question. We have confirmed with our lab and according to internal isotype testing this antibody, utrophin (8A4): sc-33700 is a mouse monoclonal IgG1 (kappa light chain). This antibody is also available directly conjugated to HRP (sc-33700 HRP), phycoerythrin (sc-33700 PE), fluoroscein (sc-33700 FITC), Alexa Fluor® 488 (sc-33700 AF488) or Alexa Fluor® 647 (sc-33700 AF647), if you want to use the antibody in a multi-antibody staining application, and avoid any issues related to using a secondary antibody for detection.
Answered by: Technical Support
Date published: 2017-08-03

could you tell me which amino acides Utrophin (8A4) against for, or which part of utrophin for example, C-terminal, N-terminal, or others of utrophin? could you write similar information like utrophin Sc-81557, see  data-sheet. utrophin (MANCHO7)

Asked by: yafeng song
Thank you for your question. Upon further review in the literature, it does appear that this monoclonal antibody has been epitope mapped. This mouse monoclonal, utrophin Antibody (8A4): sc-33700, has an epitope mapping between amino acids 3232-3273 of human origin.
Answered by: Technical Support
Date published: 2017-02-11

Can this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, this utrophin antibody sc-33700 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 5 out of 5 by from Great qualityI didn't have much expectation in WB for a protein of this size but it works very well ! I used it with HBMEC (human brain microvascular endothelial cell) protein extract and I got a great signal !
Date published: 2022-02-24
Rated 5 out of 5 by from works fine in WB and IPwe were given a sample to try it out. It works fine in WB (final dilution 1:2000 to get 1ug/ml), for blocking I used 5% milk in TBST. It works fine in IP too, I used 1ug of AB and 2mg of lysate per IP. Since utrophin is a very high molecular weight protein, I used 6% SDS-PAGE gel to resolve it.
Date published: 2018-08-27
Rated 5 out of 5 by from Great monoclonal antibodyGreat antibody for WB and IHC(P). Successful results produced with this antibody
Date published: 2017-01-17
Rated 5 out of 5 by from Great productStrong results for multiple applications including wb, if and ihc staining
Date published: 2017-01-01
Rated 5 out of 5 by from good antibodythis antibody is perfect used in western blot，especially cell lysate。
Date published: 2016-10-27
Rated 5 out of 5 by from Publishable Western blot results using mouse liverPublishable Western blot results using mouse liver, brain, kidney, heart, triceps, and quadriceps. -SCBT Publication Review
Date published: 2015-06-05
Rated 5 out of 5 by from Great antibody for use in IHCGreat antibody for use in IHC. We have purchased it again.
Date published: 2015-06-05
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with human BJAB B lymphoma cells. -SCBT Publication Review
Date published: 2015-06-01
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utrophin 抗体 (8A4) is rated 4.7 out of 5 by 12.
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