Date published: 2025-9-8
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uPAR Antibody (E-3) - Western Blotting - Image 379356
uPAR 抗体 (E-3): sc-376494. HUV-EC-C (A), NCI-H1299 (B) 和 HeLa (C) 全细胞裂解液中 uPAR 表达的 Western 印迹分析.
uPAR 抗体 (E-3): sc-376494. HUV-EC-C (A), NCI-H1299 (B) 和 HeLa (C) 全细胞裂解液中 uPAR 表达的 Western 印迹分析.

uPAR 抗体 (E-3): sc-376494

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说明书
  • uPAR 抗体 E-3 是小鼠单克隆 IgG2a κ，uPAR抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长uPAR的氨基酸1-290
  • uPAR 抗体 (E-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 uPAR
  • 抗uPAR抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取uPAR (E-3): sc-376494的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是uPAR Antibody (E-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与uPAR Antibody (E-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
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研究信息

関連項目

uPAR抗体（E-3）是一种IgG2a κ小鼠单克隆uPAR抗体（也称为PLAUR抗体、纤溶酶原激活剂、尿激酶受体抗体、尿激酶纤溶酶原激活剂受体抗体或尿激酶纤溶酶原激活剂受体抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测人类来源的uPAR蛋白。uPAR抗体（E-3）既有非偶联形式的抗uPAR抗体，也有多种偶联形式的抗uPAR抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。尿激酶纤溶酶原激活剂受体（uPAR），也称为CD87，是一种针对尿激酶纤溶酶原激活剂（uPA）的糖蛋白I锚定表面受体。与uPAR结合后，uPA将表面结合的大血清β-球蛋白、纤溶酶原转化为纤溶酶。纤溶酶，也被称为纤维蛋白溶酶，是一种类胰蛋白酶，作用于Arg-Lys键，诱导纤维蛋白和纤维蛋白原的细胞周围蛋白水解，从而有助于系统激活凝血级联反应。在病变再上皮化、伤口愈合和组织重塑过程中观察到这一途径。已知uPA和uPAR在间充质和上皮来源的肿瘤细胞中过度表达，并且是肿瘤侵袭和转移所必需的。Ras、MEK、ERK和MLCK作为uPAR依赖性信号级联的下游效应物，由uPA结合引发，并以整合素选择性的方式促进细胞迁移。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

uPAR 抗体 (E-3)

sc-376494
200 µg/ml
RMB2377.00

uPAR (E-3): m-IgG Fc BP-HRP 套装

sc-529881
200 µg Ab; 10 µg BP
RMB2662.00

uPAR (E-3): m-IgGκ BP-HRP 套装

sc-522974
200 µg Ab, 40 µg BP
RMB2662.00

uPAR (E-3): m-IgG2a BP-HRP 套装

sc-547397
200 µg Ab; 10 µg BP
RMB2662.00

uPAR 抗体 (E-3) AC

sc-376494 AC
500 µg/ml, 25% agarose
RMB3129.00

uPAR 抗体 (E-3) HRP

sc-376494 HRP
200 µg/ml
RMB2377.00

uPAR 抗体 (E-3) FITC

sc-376494 FITC
200 µg/ml
RMB2482.00

uPAR 抗体 (E-3) PE

sc-376494 PE
200 µg/ml
RMB2580.00

uPAR 抗体 (E-3) Alexa Fluor® 488

sc-376494 AF488
200 µg/ml
RMB2685.00

uPAR 抗体 (E-3) Alexa Fluor® 546

sc-376494 AF546
200 µg/ml
RMB2685.00

uPAR 抗体 (E-3) Alexa Fluor® 594

sc-376494 AF594
200 µg/ml
RMB2685.00

uPAR 抗体 (E-3) Alexa Fluor® 647

sc-376494 AF647
200 µg/ml
RMB2685.00

uPAR 抗体 (E-3) Alexa Fluor® 680

sc-376494 AF680
200 µg/ml
RMB2685.00

uPAR 抗体 (E-3) Alexa Fluor® 790

sc-376494 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for uPAR (E-3): sc-376494?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-376494.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2018-12-20
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Rated 1 out of 5 by from Very weak band in WBI use 293,HC67, Hela, SW1990,BXPC3,PANC1 cell lysis, delution 1:300.very weak band with a long exposure time. Besides, these bands almost similar.But many ref reported that 293 and HC67 do not express uPAR. Therefore,I think these weak bands are not uPAR. I wonder how these people commenting here get their results using this garbage antibody.
Date published: 2018-12-06
Rated 5 out of 5 by from Great product!!It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from good!ウエスタンブロットできれいなバンドが検出できた。 低酸素環境で発現が有意に上昇を認め、求めていた結果となった。
Date published: 2017-06-16
Rated 1 out of 5 by from No bands seenWestern blotting was performed using twelve different mouse osteosarcoma cell lines which were previously confirmed to express uPAR gene based on RT-PCR. Conditions: 12% gel electrophoresis, wet transfer over 2 hours, primary antibodies at 1:200 dilution in 5% mild blocking solution (4C overnight). There was no band visible following the protocol, although positive control (b-actin) was seen.
Date published: 2017-03-08
Rated 5 out of 5 by from Nice resultsNice results obtained with uPAR Antibody (E-3) by WB and IHCP
Date published: 2017-01-21
Rated 5 out of 5 by from Good cytoplasmic staining with paraffinGood cytoplasmic staining with paraffin-embedded human fallopian tube tissue. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Great mono antibody for detecting successfulGreat mono antibody for detecting successful uPAR activation in sc-400666-ACT transfected cells. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-01-05
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uPAR 抗体 (E-3) is rated 4.0 out of 5 by 8.
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