Date published: 2025-9-7

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ULBP4 抗体 (6E6): sc-53133

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说明书
  • ULBP4 抗体 6E6 是小鼠单克隆 IgG2b κ,ULBP4抗体, 在11篇文献中引用,规格为200 µg/ml
  • 针对纯化的human ULBP4
  • ULBP4 抗体 (6E6) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测human 来源的 ULBP4
  • 抗ULBP4抗体(6E6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物阻断剂,sc-53133 L,200 µg/0.1 ml
  • 关于如何获取ULBP4 (6E6): sc-53133免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 ULBP4 抗体 (6E6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ULBP4 抗体 (6E6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

ULBP4抗体(6E6)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测人源ULBP4蛋白。ULBP4 (6E6) 抗体有非结合型和多种结合型可供选择,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ULBP4是UL16结合蛋白家族的一员,在免疫反应中起着至关重要的作用,它充当激活受体NKG2D/DAP10的配体,而激活受体NKG2D/DAP10对于激活自然杀伤(NK)细胞和CD8+ T细胞至关重要。ULBP4和NKG2D/DAP10之间的相互作用会引发一连串信号传导事件,导致细胞因子(如干扰素-γ)的分泌,并促进肿瘤细胞的溶解。ULBP4以糖基磷脂酰肌醇(GPI)锚定蛋白的形式存在,定位于细胞表面,有助于免疫监控。ULBP4在受压或转化细胞上的表达可作为免疫识别的信号,使ULBP4成为人体抵御恶性肿瘤和感染的关键组成部分。ULBP4的可溶性形式可通过诱导蛋白酪氨酸磷酸化并激活关键信号通路(包括Janus激酶2、STAT5和磷脂酰肌醇3激酶/Akt)来调节免疫反应,进一步强调了ULBP4在免疫调节中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ULBP4 抗体 (6E6) 参考文献:

  1. 新型 MHC I 类相关分子 ULBPs 与 CMV 糖蛋白 UL16 结合,并通过 NKG2D 受体刺激 NK 细胞毒性。  |  Cosman, D., et al. 2001. Immunity. 14: 123-33. PMID: 11239445
  2. ULBP1, 2, 3:与人类巨细胞病毒糖蛋白 UL16 结合并激活 NK 细胞的新型 MHC I 类相关分子。  |  Kubin, M., et al. 2001. Eur J Immunol. 31: 1428-37. PMID: 11465099
  3. 人类 NKG2D 与其配体 MICA, MICB 和小鼠 RAE-1 蛋白家族同源物的相互作用。  |  Steinle, A., et al. 2001. Immunogenetics. 53: 279-87. PMID: 11491531
  4. UL16 结合蛋白是一种新型 MHC I 类相关蛋白,它与 NKG2D 结合并激活原代 NK 细胞中的多种信号通路。  |  Sutherland, CL., et al. 2002. J Immunol. 168: 671-9. PMID: 11777960

订购信息

产品名称产品编号规格价格数量收藏夹

ULBP4 抗体 (6E6)

sc-53133
200 µg/ml
RMB2377.00

ULBP4 (6E6): m-IgG Fc BP-HRP 套装

sc-528552
200 µg Ab; 10 µg BP
RMB2662.00

ULBP4 (6E6): m-IgGκ BP-HRP 套装

sc-520951
200 µg Ab, 40 µg BP
RMB2662.00

ULBP4 (6E6): m-IgG2b BP-HRP 套装

sc-550051
200 µg Ab; 10 µg BP
RMB2662.00

ULBP4 抗体 (6E6) L

sc-53133 L
200 µg/0.1 ml
RMB2377.00

ULBP4 抗体 (6E6) AC

sc-53133 AC
500 µg/ml, 25% agarose
RMB3129.00

ULBP4 抗体 (6E6) HRP

sc-53133 HRP
200 µg/ml
RMB2377.00

ULBP4 抗体 (6E6) FITC

sc-53133 FITC
200 µg/ml
RMB2482.00

ULBP4 抗体 (6E6) PE

sc-53133 PE
200 µg/ml
RMB2580.00

ULBP4 抗体 (6E6) Alexa Fluor® 488

sc-53133 AF488
200 µg/ml
RMB2685.00

ULBP4 抗体 (6E6) Alexa Fluor® 546

sc-53133 AF546
200 µg/ml
RMB2685.00

ULBP4 抗体 (6E6) Alexa Fluor® 594

sc-53133 AF594
200 µg/ml
RMB2685.00

ULBP4 抗体 (6E6) Alexa Fluor® 647

sc-53133 AF647
200 µg/ml
RMB2685.00

ULBP4 抗体 (6E6) Alexa Fluor® 680

sc-53133 AF680
200 µg/ml
RMB2685.00

ULBP4 抗体 (6E6) Alexa Fluor® 790

sc-53133 AF790
200 µg/ml
RMB2685.00

I bought the L form of this antibody, sc-53133 L for blocking (neutralization). How long can I store this at in the refridgerator?

Asked by: jenniferc
The L form of the antibody should be stored at -20°C. Once thawed, we recommend using as soon as possible, although it is usually stable for up to 2 weeks at 4 degrees.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specific strong signalAb anti-ULBP4 (6E6) sc-53133 The antibody was used by Western blot technique. Western blot conditions: Blocking: 5% milk 1h RT Primary antibody: 1:500 1% BSA Overnight 4°C Secondary Antibody: 1:3000 5% Milk 1h RT. Expected molecular weight: 30 KDa Observed lanes at 30 KDa By using the antibody with these conditions in melanoma cells, it works very well!
Date published: 2024-01-23
Rated 1 out of 5 by from FACS on HFFThis antibody does not work in Flow cytometry on HFF cells even at dilution 1:50, no signal can be detected
Date published: 2018-11-01
Rated 4 out of 5 by from Published data for Flow Cytometry for usePublished data for Flow Cytometry for use in human NK cells. -SCBT Publication Review
Date published: 2015-05-09
Rated 4 out of 5 by from Produced robust immunoperoxidase nuclearProduced robust immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human cervix tissue. -SCBT QC
Date published: 2015-02-03
Rated 4 out of 5 by from Publishable data for immunoelectron microscopyPublishable data for immunoelectron microscopy using human placenta. -SCBT QC
Date published: 2015-01-22
Rated 4 out of 5 by from Single strong band at correct mw in humanSingle strong band at correct mw in human skin tissue extract. -SCBT QC
Date published: 2015-01-22
Rated 4 out of 5 by from Produced great indirect FCM data for MelProduced great indirect FCM data for Mel mock and Mel-ULBP4 transfectants stained with ULBP4 -SCBT QC
Date published: 2013-09-05
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ULBP4 抗体 (6E6) is rated 3.7 out of 5 by 7.
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