Date published: 2025-9-7
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UCP2 Antibody (G-6) - Western Blotting - Image 379498
UCP2 抗体 (G-6): sc-390189. Daudi (A) 和 U-698-M (B) 全细胞裂解液中 UCP2 表达的 Western 印迹分析.
UCP2 抗体 (G-6): sc-390189. Daudi (A) 和 U-698-M (B) 全细胞裂解液中 UCP2 表达的 Western 印迹分析.

UCP2 抗体 (G-6): sc-390189

4.0(27)
寫評論提問

说明书
  • UCP2 抗体 G-6 是小鼠单克隆 IgM κ，UCP2抗体, 在32篇文献中引用，规格为200 µg/ml
  • 与氨基酸序列N-terminus（h）对应的多肽序列（UCP2的human起源）
  • 推荐用于 mouse, rat 和 human 来源的UCP2 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 关于如何获取UCP2 (G-6): sc-390189的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成UCP2 抗体 (G-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 UCP 抗体 包括 UCP1, UCP2和UCP4

UCP2抗体（G-6）是一种IgM κ小鼠单克隆UCP2抗体（也称为线粒体解偶联蛋白2抗体、SLC25A8抗体或溶质载体家族25成员8抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的UCP2蛋白。UCP2抗体（G-6）可作为非偶联的抗UCP2抗体使用。解偶联蛋白UCP1（以前称为UCP）是棕色脂肪组织线粒体特有的整体膜蛋白。UCP1形成二聚体，作为质子通道，可以通过消散线粒体内膜两侧的电化学势来解偶联氧化磷酸化。这一过程诱导棕色脂肪组织产生热量，并参与调节体温和葡萄糖代谢。UCP2是一种结构相关蛋白，也可解偶联线粒体呼吸。与UCP1相比，它在人和鼠的组织中表达更广泛，包括白色脂肪组织和肌肉。UCP2被认为在体重调节中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

UCP2 抗体 (G-6) 参考文献:

  1. 线粒体解偶联蛋白 UCP2 介导的过氧化氢脂肪酸循环。  |  Jaburek, M., et al. 2004. J Biol Chem. 279: 53097-102. PMID: 15475368
  2. 异质核糖核蛋白 K 可增强胰岛素诱导的线粒体 UCP2 蛋白的表达。  |  Ostrowski, J., et al. 2004. J Biol Chem. 279: 54599-609. PMID: 15485813
  3. 过表达短异源二聚体伙伴可恢复过表达 UCP2 的胰腺β细胞在葡萄糖刺激下受损的胰岛素分泌。  |  Suh, YH., et al. 2004. J Endocrinol. 183: 133-44. PMID: 15525581
  4. 棕色脂肪组织产热：跨学科研究。  |  Himms-Hagen, J. 1990. FASEB J. 4: 2890-8. PMID: 2199286
  5. 人类解偶联蛋白基因：结构, 与大鼠基因的比较以及归属于第 4 号染色体长臂。  |  Cassard, AM., et al. 1990. J Cell Biochem. 43: 255-64. PMID: 2380264
  6. 小鼠棕色脂肪线粒体解偶联蛋白。分子克隆, 基因图谱和 mRNA 表达。  |  Jacobsson, A., et al. 1985. J Biol Chem. 260: 16250-4. PMID: 2999153
  7. 棕色脂肪的生热机制  |  Nicholls, DG. and Locke, RM. 1984. Physiol Rev. 64: 1-64. PMID: 6320232
  8. 解偶联蛋白-2：与肥胖和高胰岛素血症有关的新基因。  |  Fleury, C., et al. 1997. Nat Genet. 15: 269-72. PMID: 9054939

订购信息

产品名称产品编号规格价格数量收藏夹

UCP2 抗体 (G-6)

sc-390189
200 µg/ml
RMB2377.00

UCP2 (G-6) 中和勝肽

sc-390189 P
100 µg/0.5 ml
RMB511.00

Hello, could you please send me the exact sequence of the N-terminal epitope which was used to generate the antibody? The reason is that I would like to try it on cells from the Xenopus frog, and therefore a good sequence conservation is required.

Asked by: Sylvain_M
Thank you for your question . The sequence is proprietary and the homology in xenopus 63%. We only warranty a specie if the homology is 95% or higher .
Answered by: BlakeJ
Date published: 2023-04-24

please i want to know if his kit of ucp2 (Cat. # sc-390189 ) measure mitochondrial ucp2

Asked by: dr asmaa gomaa
Thank you for your question. This is not provided as a kit. This is a mouse monoclonal primary antibody recommended for detection of UCP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and indirect ELISA. UCP2 is also known as Mitochondrial uncoupling protein 2. A link to the Uniprot protein page about UCP2 is provided below for your reference. https://www.uniprot.org/uniprot/P55851
Answered by: Technical Support
Date published: 2024-03-13

I am interested for a free sample of the above product. (UCP2 Antibody (G-6): sc-390189)

Asked by: Mohasin
Thank you very much for your inquiry. We don't have currently a limit in the samples we can send per order, would you, or any of your colleagues, like to try any other antibody? please let me know so we make a single shipment. Please let me know the following information: - complete address for shipping - telephone, fax number and e-mail address - a contact person the products should be addressed to - catalogue number and product description Remember once you try the sample, you can write a review in our web about it, indicating all the information you think might be useful for other researchers.
Answered by: Technical Support Europe
Date published: 2017-09-21

The blot for WI-38 cells shows 2 bands, 1 band nearly 80 kDa. This band is also labeled as UCP2, is it right? If yes, what ist the explanation for this band? And could it be that only the 80kDa band ist detectabel without the lower band?

Asked by: Rene 42
Thank you for your question. We have observed a higher molecular weight band ~70-80 kDa in both WI-38 and Neuro-2A whole cell lysate, as well as human liver tissue extract using this monoclonal antibody, UCP2 (G-6): sc-390189, in Western blot. UCP2 has been reported in the primary literature as a dimer at this molecular weight. As a reference, please see PMID: 11179956.
Answered by: Technical Support
Date published: 2017-05-17

What is the total volume provided of UCP2 antibody (G-6)?

Asked by: AbPolly
Each vial contains 200 µg IgM in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 4 out of 5 by from Good!It works pretty well in liver homogenates form steatotic livers.
Date published: 2022-03-24
Rated 5 out of 5 by from UCP2 (G-6) antibodyThis antibody is perfect for cell culture samples because of the one band that you can see in the membrane. Very clean antibody.
Date published: 2021-06-19
Rated 4 out of 5 by from good!Bande nitide. L'anticorpo funziona bene (1:100 PBST 1%)
Date published: 2021-04-05
Rated 2 out of 5 by from Not recommended for mouse samplesStrong non-specific bands appeared in WCE of mouse cell lines. The result was not consistent with qRT-PCR. Dilution: 1:100 in HIKARI sol. (Nacalai, Japan)
Date published: 2021-04-05
Rated 5 out of 5 by from UCP2 antibodyVery clean and useful antibody, it works really good for Western blotting.
Date published: 2021-02-10
Rated 5 out of 5 by from ottimo funzionamentosono rimasta soddisfatta dell' anticorpo UCP2 (G-6)
Date published: 2020-11-18
Rated 2 out of 5 by from It doesn't work well.We used it at 1:100 dilution in BSA on human cell line. Unfortunately we had a lot of problem to detect it.
Date published: 2020-10-13
Rated 5 out of 5 by from worked greatgood specificity and no back ground with tissue and cell 1 st *5000 2nd *5000
Date published: 2018-01-30
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UCP2 抗体 (G-6) is rated 4.0 out of 5 by 27.
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