Date published: 2025-9-7
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UBF Antibody (F-9) - Western Blotting - Image 380378
UBF 抗体 (F-9): sc-13125. HL-60 (A), WEHI-231 (B) 和 NIH/3T3 (C) 核提取物中 UBF 表达的 Western 印迹分析.
UBF 抗体 (F-9): sc-13125. HL-60 (A), WEHI-231 (B) 和 NIH/3T3 (C) 核提取物中 UBF 表达的 Western 印迹分析.

UBF 抗体 (F-9): sc-13125

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说明书
  • UBF 抗体 F-9 是小鼠单克隆 IgG1 κ，UBF抗体, 在206篇文献中引用，规格为200 µg/ml
  • 免疫human物种的UBF的氨基酸1-220
  • UBF 抗体 (F-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 UBF; 也和以下物种反应，包括： 和 equine, canine and porcine
  • 抗UBF抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13125 X, 200 µg/0.1 ml)
  • 关于如何获取UBF (F-9): sc-13125的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 UBF 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与UBF 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

UBF抗体（F-9）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人源UBF蛋白。UBF (F-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。UBF蛋白在RNA聚合酶I转录核糖体RNA基因的过程中起着至关重要的作用，这一过程与细胞的生长状态密切相关。UBF通过与核糖体RNA基因启动子和增强子区域内的特定DNA元件结合，充当转激活因子，促进RNA聚合酶I进入启动子复合物。这种相互作用对于核糖体RNA合成的正确调节至关重要，对于细胞中的核糖体生物发生和蛋白质合成必不可少。UBF因mRNA剪接而存在两种形式，UBF活性受酪蛋白激酶II在羧基末端磷酸化调节，也受与视网膜母细胞瘤易感基因产物Rb等其他蛋白的相互作用调节。当UBF不与E2F家族成员结合时，Rb可抑制UBF活性。UBF的调节对于维持细胞生长和增殖所需的核糖体RNA生成平衡至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

UBF 抗体 (F-9) 参考文献:

  1. Rb 和 p130 调节 RNA 聚合酶 I 的转录：Rb 破坏了 UBF 和 SL-1 之间的相互作用。  |  Hannan, KM., et al. 2000. Oncogene. 19: 4988-99. PMID: 11042686
  2. 核糖体 RNA 基因启动子上启动前复合体组装之后的一个步骤限制了人类 RNA 聚合酶 I 依赖性转录的速率。  |  Panov, KI., et al. 2001. Mol Cell Biol. 21: 2641-9. PMID: 11283244
  3. 核极转录因子 mUBF 在 C 端超酸性尾部被酪蛋白激酶 II 磷酸化，这是转录激活所必需的。  |  Voit, R., et al. 1992. EMBO J. 11: 2211-8. PMID: 1600946
  4. UBF 的乙酰化在细胞周期中发生变化，并调节 UBF 与 RNA 聚合酶 I 的相互作用。  |  Meraner, J., et al. 2006. Nucleic Acids Res. 34: 1798-806. PMID: 16582105
  5. UBF通过刺激启动子逃逸激活RNA聚合酶I的转录。  |  Panov, KI., et al. 2006. EMBO J. 25: 3310-22. PMID: 16858408
  6. 质谱鉴定 rRNA 转录因子上游结合因子的磷酸化位点。  |  Lin, CH., et al. 2007. Am J Physiol Cell Physiol. 292: C1617-24. PMID: 17182730
  7. UBF在调节转录活性rDNA染色质结构和动力学中的作用。  |  Sanij, E. and Hannan, RD. 2009. Epigenetics. 4: 374-82. PMID: 19717978
  8. 核极转录因子 hUBF 含有与 HMG 蛋白同源的 DNA 结合基序。  |  Jantzen, HM., et al. 1990. Nature. 344: 830-6. PMID: 2330041
  9. 小鼠细胞中转录因子UBF的缺失导致rRNA转录网络下游和上游元件的下调。  |  Theophanous, A., et al. 2023. J Biol Chem. 299: 105203. PMID: 37660911
  10. 核极转录激活剂 UBF 可缓解 Ku 抗原介导的小鼠核糖体基因转录抑制。  |  Kuhn, A., et al. 1993. Nucleic Acids Res. 21: 2057-63. PMID: 8502546
  11. 90千道尔顿的核仁组织区自身抗原（上游结合因子）的两种异构体与抗肿瘤药物顺铂修饰的DNA结合的热敏性不同。  |  Codony-Servat, J., et al. 1996. Biochem Pharmacol. 51: 1131-6. PMID: 8645334
  12. RNA 聚合酶 I 对哺乳动物核糖体基因转录的调控。  |  Grummt, I. 1999. Prog Nucleic Acid Res Mol Biol. 62: 109-54. PMID: 9932453

订购信息

产品名称产品编号规格价格数量收藏夹

UBF 抗体 (F-9)

sc-13125
200 µg/ml
RMB2377.00

UBF (F-9): m-IgG Fc BP-HRP 套装

sc-528264
200 µg Ab; 10 µg BP
RMB2662.00

UBF (F-9): m-IgGκ BP-HRP 套装

sc-520597
200 µg Ab, 40 µg BP
RMB2662.00

UBF (F-9): m-IgG1 BP-HRP 套装

sc-542847
200 µg Ab; 20 µg BP
RMB2662.00

UBF 抗体 (F-9) X

sc-13125 X
200 µg/0.1 ml
RMB2377.00

UBF 抗体 (F-9) AC

sc-13125 AC
500 µg/ml, 25% agarose
RMB3129.00

UBF 抗体 (F-9) HRP

sc-13125 HRP
200 µg/ml
RMB2377.00

UBF 抗体 (F-9) FITC

sc-13125 FITC
200 µg/ml
RMB2482.00

UBF 抗体 (F-9) PE

sc-13125 PE
200 µg/ml
RMB2580.00

UBF 抗体 (F-9) Alexa Fluor® 488

sc-13125 AF488
200 µg/ml
RMB2685.00

UBF 抗体 (F-9) Alexa Fluor® 546

sc-13125 AF546
200 µg/ml
RMB2685.00

UBF 抗体 (F-9) Alexa Fluor® 594

sc-13125 AF594
200 µg/ml
RMB2685.00

UBF 抗体 (F-9) Alexa Fluor® 647

sc-13125 AF647
200 µg/ml
RMB2685.00

UBF 抗体 (F-9) Alexa Fluor® 680

sc-13125 AF680
200 µg/ml
RMB2685.00

UBF 抗体 (F-9) Alexa Fluor® 790

sc-13125 AF790
200 µg/ml
RMB2685.00

Is the antibody(UBF Antikörper (F-9): sc-13125) suited for ChIP aswell?

Asked by: Katinka
Thanks for your question.TransCruz reagent (sc-13125 X, 200 µg/0.1 ml) is recommended for ChIP applications.
Answered by: Technical Support 9
Date published: 2020-06-14

Why would you recommend using this mouse monoclonal sc-13125: UBF (F-9) over the now discontinued rabbit polyclonal sc-9131 UBF (H-300)?

Asked by: cjMara
Thank you for your question. This mouse monoclonal antibody is raised against the identical recombinant protein immunogen used to generate the rabbit polyclonal (sc-9131). This monoclonal antibody offers improved specificity in the same applications, without any lot to lot variability. The antibody has published data in WB, IF and IHC(P). And it has been featured in twice as many publications than the polyclonal.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 5 out of 5 by from Worked with co-IFThis worked well and gave us signal in our co-IF experiment.
Date published: 2025-04-08
Rated 2 out of 5 by from Anticorpo buono per alcune applicazioniQuesto anticorpo funziona molto bene in Western Blot, ha una buona resa, pulita da bande aspecifiche. Invece per quanto riguarda l' applicazione per IF personalmente ho sempre ottenuto segnale scarso, con molto marcaggio aspecifico. Ho notato segnale scarso e aspecifico anche provando ad utilizzarlo in immunoistochimica applicata a microscopia elettronica a trasmissione.
Date published: 2020-11-13
Rated 5 out of 5 by from good for tissue staininggood for tissue staining, used 1:50 tho. asked for a sample went well, so we ordered one
Date published: 2019-09-25
Rated 5 out of 5 by from Works very well in IFWorks very well in IF on human fibroblast cells both with MeOH and PFA fixation.
Date published: 2018-07-26
Rated 5 out of 5 by from excellent productWorks great by immunoFISH, supplied in large quantity.
Date published: 2017-12-08
Rated 3 out of 5 by from WB 1:500, 3 distinguished bands in KM12Working fine at dilution 1:500 in KM12 but 3 distinguished bands visible in the given range of protein molecular weight.
Date published: 2017-10-11
Rated 5 out of 5 by from an excellent antibody for both WB and IFThis antibody works very well for WB (1:500 dilution, 40 ug HeLa or U251 Lysate) and IF (1:500 dilution, formaldehyde-fixed HeLa cells)
Date published: 2017-07-05
Rated 5 out of 5 by from 非常好用WB，chip-qPCR和IF都很好用;抗体特异性不错，量多，一支可以满足多种实验的需求；性价比高，推荐大家使用
Date published: 2017-06-14
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UBF 抗体 (F-9) is rated 4.4 out of 5 by 21.
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