Date published: 2025-9-8

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UBE1 抗体 (2G2): sc-53555

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说明书
  • UBE1 抗体 2G2 是小鼠单克隆 IgG1 κ,UBE1抗体, 在8篇文献中引用,规格为200 µg/ml
  • 针对泛素激活酶E1
  • UBE1 抗体 (2G2) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 UBE1
  • 抗UBE1抗体(2G2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取UBE1 (2G2): sc-53555免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 UBE1 抗体 (2G2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与UBE1 抗体 (2G2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

UBE1 抗体(2G2)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠、大鼠和人源的 UBE1 蛋白。抗 UBE1 抗体 (2G2) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。泛素活化酶 E1(UBE1)在泛素-蛋白酶体途径中起着至关重要的作用,而泛素-蛋白酶体途径对于调节蛋白质降解和维持细胞稳态至关重要。UBE1 以 ATP 依赖性方式腺嘌呤化泛素 C 端甘氨酸残基,与 UBE1 上的半胱氨酸残基形成硫酯键,催化泛素连接的第一步。激活的泛素随后被转移到泛素结合酶上,然后泛素结合酶将泛素分子捐献给目标底物,使其降解。UBE1 的正常功能至关重要,因为这一途径的失调会导致各种疾病,包括癌症和神经退行性疾病。值得注意的是,UBE1基因是X-Y同源基因的一个例子,在许多哺乳动物中,它是X连锁的,但在人类的Y染色体上没有发现UBE1同源基因。据信,UBE1 在人类中可避免 X 失活,这可能会对雌性基因的剂量和表达产生影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

UBE1 抗体 (2G2) 参考文献:

  1. 小鼠泛素激活酶 E1(Ube1)基因的图谱绘制和表达。  |  Disteche, CM., et al. 1992. Mamm Genome. 3: 156-61. PMID: 1617221
  2. 人类泛素激活酶 E1 的分子克隆, 序列和组织分布。  |  Handley, PM., et al. 1991. Proc Natl Acad Sci U S A. 88: 258-62. PMID: 1986373
  3. 泛素E1酶Ube1在不同压力条件下介导NEDD8的激活。  |  Leidecker, O., et al. 2012. Cell Cycle. 11: 1142-50. PMID: 22370482
  4. 泛素激活酶UBE1与泛素样修饰酶FAT10的结合使其成为蛋白酶体降解的目标。  |  Bialas, J., et al. 2015. PLoS One. 10: e0120329. PMID: 25768649
  5. 泛素激活酶E1作为治疗再狭窄的新治疗靶点。  |  Qin, Z., et al. 2016. Atherosclerosis. 247: 142-53. PMID: 26919560
  6. 泛素活化酶 E1 抑制剂PYR-41 可延缓精子与卵子融合后的增大。  |  Yoshida, K., et al. 2018. Reprod Toxicol. 76: 71-77. PMID: 29355596
  7. 泛素激活酶E1(UBA1)在神经元健康和神经变性中的关键作用。  |  Lambert-Smith, IA., et al. 2020. Int J Biochem Cell Biol. 123: 105746. PMID: 32315770
  8. 姜黄素能部分阻止 ISG15 通过泛素活化酶 E1-like 蛋白活化,并减少 ISGylation。  |  Oki, N., et al. 2022. Biochem Biophys Res Commun. 625: 94-101. PMID: 35952613
  9. 一个名为 DXS8237E 的新基因位于 Xp11.23 的 UBE1 上游 20 kb 范围内,其 X 失活状态不同。  |  Coleman, MP., et al. 1996. Genomics. 31: 135-8. PMID: 8808293
  10. 哺乳动物 Y 染色体上泛素激活酶基因的起源和丢失。  |  Mitchell, MJ., et al. 1998. Hum Mol Genet. 7: 429-34. PMID: 9467000
  11. 人和小鼠泛素激活酶 E1 和 PCTAIRE-1 基因的 X 失活分析和 DNA 甲基化研究。  |  Carrel, L., et al. 1996. Hum Mol Genet. 5: 391-401. PMID: 8852665
  12. 对候选精子发生基因 Ube1y 和与之密切相关的 Ube1x 的转录分析表明,它们在精原细胞和精母细胞中共同表达,但在青春期精母细胞中受到抑制。  |  Odorisio, T., et al. 1996. Dev Biol. 180: 336-43. PMID: 8948595

订购信息

产品名称产品编号规格价格数量收藏夹

UBE1 抗体 (2G2)

sc-53555
200 µg/ml
RMB2377.00

UBE1 (2G2): m-IgG Fc BP-HRP 套装

sc-528573
200 µg Ab; 10 µg BP
RMB2662.00

UBE1 (2G2): m-IgGκ BP-HRP 套装

sc-520984
200 µg Ab, 40 µg BP
RMB2662.00

UBE1 (2G2): m-IgG1 BP-HRP 套装

sc-543004
200 µg Ab; 20 µg BP
RMB2662.00

UBE1 抗体 (2G2) AC

sc-53555 AC
500 µg/ml, 25% agarose
RMB3129.00

UBE1 抗体 (2G2) HRP

sc-53555 HRP
200 µg/ml
RMB2377.00

UBE1 抗体 (2G2) FITC

sc-53555 FITC
200 µg/ml
RMB2482.00

UBE1 抗体 (2G2) PE

sc-53555 PE
200 µg/ml
RMB2580.00

UBE1 抗体 (2G2) Alexa Fluor® 488

sc-53555 AF488
200 µg/ml
RMB2685.00

UBE1 抗体 (2G2) Alexa Fluor® 546

sc-53555 AF546
200 µg/ml
RMB2685.00

UBE1 抗体 (2G2) Alexa Fluor® 594

sc-53555 AF594
200 µg/ml
RMB2685.00

UBE1 抗体 (2G2) Alexa Fluor® 647

sc-53555 AF647
200 µg/ml
RMB2685.00

UBE1 抗体 (2G2) Alexa Fluor® 680

sc-53555 AF680
200 µg/ml
RMB2685.00

UBE1 抗体 (2G2) Alexa Fluor® 790

sc-53555 AF790
200 µg/ml
RMB2685.00

Can UBE1 (2G2): sc-53555 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, UBE1 (2G2): sc-53555 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 内在性UBA1を確認出来ました。A549細胞を用いて確認した結果、内在性UBA1の発現を確認出来ました。シグナルも容易に検出出来ました。
Date published: 2025-04-16
Rated 5 out of 5 by from worked well for western blotThis antibody worked great for western blot on mouse heart at 1/500 dilution. Detected band around 120 kd.
Date published: 2018-04-30
Rated 5 out of 5 by from Excellent productThe UBE1 Antibody (2G2): sc-53555 works very well for immunofluorescence, showing strong staining pattern localized to the nucleus.
Date published: 2017-01-26
Rated 5 out of 5 by from Very good antibodyThis antibody nicely detects vey well UBE1 in breast cancer cell lines as analyzed by WB.
Date published: 2016-11-08
Rated 5 out of 5 by from Published western blot data using ts85 cellPublished western blot data using ts85 cell lysates (mouse mammary carcinoma cell line). -SCBT Publication Review
Date published: 2015-05-21
Rated 5 out of 5 by from Great immunoperoxidase nuclear and cytoplasmicGreat immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-05-14
Rated 5 out of 5 by from Robust bands in western blot analysis ofRobust bands in western blot analysis of UBE1 expression in HeLa nuclear extract and Hep G2 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-15
Rated 4 out of 5 by from RobustRobust, slightly overblown signal in western blot using human UBE1 transfected 293T whole cell lysates and HeLa nuclear extract. -SCBT QC
Date published: 2015-03-12
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UBE1 抗体 (2G2) is rated 4.9 out of 5 by 8.
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