Date published: 2025-9-7

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Trypsin 抗体 (D-1): sc-137077

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说明书
  • Trypsin 抗体 D-1 是小鼠单克隆 IgG2a κ,Trypsin抗体, 在14篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列39-140位于human物种的Trypsin-1的N-terminus附近
  • Trypsin 抗体 (D-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Trypsin-1, Trypsin-2 and Trypsin-3
  • 抗Trypsin抗体(D-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Trypsin (D-1): sc-137077免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Trypsin 抗体 (D-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Trypsin 抗体 (D-1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

胰蛋白酶抗体(D-1)是一种IgG2a κ小鼠单克隆胰蛋白酶抗体(也称为TRY1抗体、丝氨酸蛋白酶1抗体、阳离子胰蛋白酶原抗体、β-胰蛋白酶抗体、TRYP1抗体、TRP1抗体、TCR V Beta 4.1抗体、胰蛋白酶原1抗体、胰蛋白酶原A抗体、胰蛋白酶1抗体或胰蛋白酶I抗体),可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学(石蜡)和酶联免疫吸附试验检测小鼠、大鼠和人源的胰蛋白酶。胰蛋白酶抗体(D-1)既有非共轭形式的抗胰蛋白酶抗体,也有多种共轭形式的抗胰蛋白酶抗体,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®共轭物。人胰腺将三种不同的非活性胰蛋白酶原异构体分泌到小肠中,即阳离子胰蛋白酶原、阴离子胰蛋白酶原(两种主要异构体)和中胰蛋白酶原(一种次要异构体)。在小肠中,每种异构体都被肠激酶切割成其活性形式,分别为胰蛋白酶-1、胰蛋白酶-2和胰蛋白酶-3。所有胰蛋白酶都是丝氨酸蛋白酶胰蛋白酶家族的成员。活化的胰蛋白酶继续激活其他蛋白酶原,并在胰蛋白酶原的自动激活中发挥作用。这表明胰蛋白酶在消化中起重要作用。编码胰蛋白酶-1的基因突变刺激其活性,与常染色体显性遗传胰腺炎(HCP)有关,也称为慢性胰腺炎(CP),该病以持续、严重的腹痛为特征,由于实质钙化、胰腺结石、囊肿和胰头肿大。胰蛋白酶-3除在胰腺中表达外,还在大脑中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Trypsin 抗体 (D-1) 参考文献:

  1. 慢性胰腺炎:发病机制和分子方面。  |  Kleeff, J., et al. 2000. Ann Ital Chir. 71: 3-10. PMID: 10829517
  2. 遗传性和非遗传性慢性胰腺炎中PRSS1突变的缺失和SPINK1胰蛋白酶抑制剂突变的缔合作用。  |  Chandak, GR., et al. 2004. Gut. 53: 723-8. PMID: 15082592
  3. 糜蛋白酶 C(卡尔德克林)刺激人类阳离子胰蛋白酶原的自激活。  |  Nemoda, Z. and Sahin-Tóth, M. 2006. J Biol Chem. 281: 11879-86. PMID: 16505482
  4. 胰蛋白酶、胰蛋白酶抑制剂和胰蛋白酶受体在胰腺炎发病和恶化中的作用。  |  Hirota, M., et al. 2006. J Gastroenterol. 41: 832-6. PMID: 17048046
  5. 人类阳离子胰蛋白酶原的 Tyr154 被硫酸化。  |  Sahin-Tóth, M., et al. 2006. FEBS J. 273: 5044-50. PMID: 17087724
  6. 囊性纤维化跨膜传导调节因子(CFTR)突变/变异/单倍型与肿瘤坏死因子(TNF)启动子多态性在高脂血症性胰腺炎中的相关性。  |  Chang, YT., et al. 2008. Clin Chem. 54: 131-8. PMID: 17981921
  7. 遗传性慢性胰腺炎  |  Teich, N. and Mössner, J. 2008. Best Pract Res Clin Gastroenterol. 22: 115-30. PMID: 18206817
  8. 遗传疾病在急性复发性胰腺炎中的作用。  |  Keim, V. 2008. World J Gastroenterol. 14: 1011-5. PMID: 18286680
  9. 天冬氨酸异构化对胰蛋白酶和胰蛋白酶样蛋白水解的影响。  |  Silzel, JW., et al. 2022. Anal Chem. 94: 15288-15296. PMID: 36279259
  10. 胰蛋白酶抑制剂。  |  Bhoola, KD. 1970. Lancet. 1: 778. PMID: 4191275
  11. 中胰蛋白酶:一种新的抗抑制剂蛋白酶,来自人体胰腺组织和液体中的一种酶原。  |  Rinderknecht, H., et al. 1984. Gastroenterology. 86: 681-92. PMID: 6698368
  12. 通过二维等电聚焦/十二烷基硫酸钠凝胶电泳鉴定人类胰腺外分泌蛋白。  |  Scheele, G., et al. 1981. Gastroenterology. 80: 461-73. PMID: 6969677

订购信息

产品名称产品编号规格价格数量收藏夹

Trypsin 抗体 (D-1)

sc-137077
200 µg/ml
$316.00

Trypsin (D-1): m-IgG Fc BP-HRP 套装

sc-528854
200 µg Ab; 10 µg BP
$354.00

Trypsin (D-1): m-IgGκ BP-HRP 套装

sc-521418
200 µg Ab, 40 µg BP
$354.00

Trypsin (D-1): m-IgG2a BP-HRP 套装

sc-547191
200 µg Ab; 10 µg BP
$354.00

Trypsin 抗体 (D-1) AC

sc-137077 AC
500 µg/ml, 25% agarose
$416.00

Trypsin 抗体 (D-1) HRP

sc-137077 HRP
200 µg/ml
$316.00

Trypsin 抗体 (D-1) FITC

sc-137077 FITC
200 µg/ml
$330.00

Trypsin 抗体 (D-1) PE

sc-137077 PE
200 µg/ml
$343.00

Trypsin 抗体 (D-1) Alexa Fluor® 488

sc-137077 AF488
200 µg/ml
$357.00

Trypsin 抗体 (D-1) Alexa Fluor® 546

sc-137077 AF546
200 µg/ml
$357.00

Trypsin 抗体 (D-1) Alexa Fluor® 594

sc-137077 AF594
200 µg/ml
$357.00

Trypsin 抗体 (D-1) Alexa Fluor® 647

sc-137077 AF647
200 µg/ml
$357.00

Trypsin 抗体 (D-1) Alexa Fluor® 680

sc-137077 AF680
200 µg/ml
$357.00

Trypsin 抗体 (D-1) Alexa Fluor® 790

sc-137077 AF790
200 µg/ml
$357.00

There is a typo in the product description, it is written TrySpin and not TrypSin. "Trypsin Antibody (D-1) is recommended for detection of Tryspin-1, Tryspin-2 and Tryspin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA"

Asked by: Thyago Cardim
Thank you for contacting Santa Cruz Biotechnology. Thank you for providing this information we will have the webpage updated .
Answered by: BlakeJ
Date published: 2024-10-02

May I check the expression of trypsin-3 in Rat colonic tissue of IBS model?

Asked by: summer870211
Thank you for your question. Anti-Trypsin Antibody (D-1) is recommended for detection of Tryspin-1, Tryspin-2 and Tryspin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA so it can be used to check expression of Trypsin in rat tissue samples.
Answered by: Tech Support Europe
Date published: 2020-08-04

May i check sc-516140 is compatible with sc-137077?

Asked by: Azrina
Thank you for your question. Yes, Trypsin Antibody (D-1) is a mouse monoclonal IgG2a (kappa light chain), and therefore you can use our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to fluorescein (FITC): sc-516140, as a secondary antibody for detection of this mouse monoclonal primary antibody with a kappa light chain.
Answered by: Technical Support
Date published: 2019-03-21

What is the recommended antigen retrieval method used with Trypsin Antibody (D-1): sc-137077 in formalin-fixed paraffin-embedded tissue sections?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from Outstanding WB and IHCVery clear, strong western signal; also grea IHC results
Date published: 2017-01-26
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-09
Rated 5 out of 5 by from GreatGreat, strong band in WB in rat pancreas tissue extract. -SCBT QC
Date published: 2015-06-22
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Publishable WB data with rat lung tissuePublishable WB data with rat lung tissue - SCBT Publication Review
Date published: 2015-04-30
Rated 5 out of 5 by from IHC publishable data with human pancreaticIHC publishable data with human pancreatic tumor cells - SCBT Publication Review
Date published: 2014-04-17
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Trypsin 抗体 (D-1) is rated 5.0 out of 5 by 6.
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