Date published: 2025-9-8
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Trx Antibody (A-5) - Western Blotting - Image 72969
Trx 抗体 (A-5): sc-166393. THP-1 (A), AML-193 (B) 和 BJAB (C) 全细胞裂解液中 Trx 表达的 Western 印迹分析.
Trx 抗体 (A-5): sc-166393. THP-1 (A), AML-193 (B) 和 BJAB (C) 全细胞裂解液中 Trx 表达的 Western 印迹分析.

Trx 抗体 (A-5): sc-166393

4.4(7)
寫評論提問

说明书
  • Trx 抗体 A-5 是小鼠单克隆 IgG1 κ，Trx抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长Trx的氨基酸1-105
  • Trx 抗体 (A-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Trx
  • 抗Trx抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Trx (A-5): sc-166393的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Trx 抗体 (A-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Trx 抗体 (A-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Trx 抗体包括 Trx-2, TrxR1 和 TrxR2

Trx抗体（A-5）是一种IgG1 κ小鼠单克隆Trx抗体（也称为TXN抗体、Thioredoxin抗体、TRDX抗体、TRX抗体或TRX1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人源Trx蛋白。Trx抗体（A-5）既可以是非偶联的抗Trx抗体形式，也可以是多种偶联形式的抗Trx抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。硫氧还蛋白（Trx）是一种在多种生物体（如细菌、植物和哺乳动物）中发现的氧化还原蛋白，含有一个保守的活性位点，由Trp-Cys-Gly-Pro-Cys组成。Trx具有多种生物学功能。它作为核糖核苷酸还原酶的氢供体，这对DNA合成至关重要，并调节多种转录因子的DNA结合活性，包括NFkB、AP-1、p53、TFIIIC和糖皮质激素受体。Trx还刺激细胞生长，抑制细胞凋亡，并在抗氧化应激过程中发挥作用。抑制Trx的药物具有抗肿瘤活性，提示Trx参与多种人类疾病，包括癌症。TrxR是一种普遍表达的黄素蛋白，可催化Trx以及其他几种氧化细胞组分的NADPH依赖性还原反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Trx 抗体 (A-5)

sc-166393
200 µg/ml
$316.00

Trx (A-5): m-IgG Fc BP-HRP 套装

sc-528953
200 µg Ab; 10 µg BP
$354.00

Trx (A-5): m-IgGκ BP-HRP 套装

sc-521587
200 µg Ab, 40 µg BP
$354.00

Trx (A-5): m-IgG1 BP-HRP 套装

sc-543233
200 µg Ab; 20 µg BP
$354.00

Trx 抗体 (A-5) AC

sc-166393 AC
500 µg/ml, 25% agarose
$416.00

Trx 抗体 (A-5) HRP

sc-166393 HRP
200 µg/ml
$316.00

Trx 抗体 (A-5) FITC

sc-166393 FITC
200 µg/ml
$330.00

Trx 抗体 (A-5) PE

sc-166393 PE
200 µg/ml
$343.00

Trx 抗体 (A-5) Alexa Fluor® 488

sc-166393 AF488
200 µg/ml
$357.00

Trx 抗体 (A-5) Alexa Fluor® 546

sc-166393 AF546
200 µg/ml
$357.00

Trx 抗体 (A-5) Alexa Fluor® 594

sc-166393 AF594
200 µg/ml
$357.00

Trx 抗体 (A-5) Alexa Fluor® 647

sc-166393 AF647
200 µg/ml
$357.00

Trx 抗体 (A-5) Alexa Fluor® 680

sc-166393 AF680
200 µg/ml
$357.00

Trx 抗体 (A-5) Alexa Fluor® 790

sc-166393 AF790
200 µg/ml
$357.00

Can Trx (A-5): sc-166393 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Trx (A-5): sc-166393 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Could I use this antibody for IHC ? 

Asked by: Jelly
Thank you for your question. We have not yet tested this mouse monoclonal, Trx Antibody (A-5): sc-166393, on formalin-fixed paraffin-embedded tissues (FFPE). We currently recommend and warranty the antibody for Western blot and IF, or immunofluorescent staining on fresh or frozen tissues or cells fixed in methanol or acetone. We do offer an alternative antibody that is recommended for IHC on FFPE, our mouse monoclonal, Trx Antibody (3A1): sc-58440. (https://www.scbt.com/scbt/product/trx-antibody-3a1)
Answered by: Technical Support
Date published: 2017-02-02
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Rated 5 out of 5 by from Clear band without backgroundI got this sample, and tried it for my blot. It worked great at 1:500 dilution. I am planning to do IF with this antibody.
Date published: 2018-10-23
Rated 5 out of 5 by from Great signal for western blottingI have used this antibody in a 1:250 dilution for western blotting using glioblastoma and breast cancer cell lines and obtained a great signal
Date published: 2017-07-17
Rated 4 out of 5 by from 也可以跑出rat来源的，只是效价不高我用这个抗体跑rat来源的蛋白，效价不太高，可能跑人源的话会更好，因为抗体说明是说的推荐用于人源的检测
Date published: 2017-07-05
Rated 4 out of 5 by from Works in rat tissue samplesI got 2 strong bands at around 20 kDa, and additional weaker band at 26 kDA.
Date published: 2017-05-11
Rated 5 out of 5 by from Great productThe Trx Antibody (A-5): sc-166393 works very well for Western blot applications, showing strong and clear banding with minimal background noise present.
Date published: 2017-02-06
Rated 3 out of 5 by from Robust bands seen in WB using THPRobust bands seen in WB using THP-1, AML-193 and BJAB whole cell lysates. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from WB publishable data with mouse RAW 264WB publishable data with mouse RAW 264.7 - SCBT Publication Review
Date published: 2013-02-15
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Trx 抗体 (A-5) is rated 4.4 out of 5 by 7.
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