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TRP2/DCT Antibody (C-9) - Western Blotting - Image 376985
TRP2 抗体 (C-9): sc-74439. Y79 (A), K-562 (B), HeLa (C), NCI-H460 (D), MCF7 (E) 和 C6 (F) 全细胞裂解液中 TRP2 表达的 Western 印迹分析.
TRP2 抗体 (C-9): sc-74439. Y79 (A), K-562 (B), HeLa (C), NCI-H460 (D), MCF7 (E) 和 C6 (F) 全细胞裂解液中 TRP2 表达的 Western 印迹分析.

TRP2/DCT 抗体 (C-9): sc-74439

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寫評論提問

说明书
  • TRP2/DCT 抗体 C-9 是小鼠单克隆 IgG2a κ，TRP2/DCT抗体, 在57篇文献中引用，规格为200 µg/ml
  • 免疫human物种的TRP2的氨基酸41-190
  • TRP2/DCT 抗体 (C-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TRP2
  • 抗TRP2/DCT抗体（C-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRP2/DCT (C-9): sc-74439的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 TRP2/DCT 抗体 (C-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TRP2/DCT 抗体 (C-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Tyrosinase Related Protein 抗体 包括 TRP2

TRP2抗体（C-9）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的TRP2蛋白。TRP2 (C-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。TRP2，也称为多巴色氨酸异构酶，在黑色素生物合成途径中起着至关重要的作用，而该途径对于脊椎动物的色素沉着至关重要。TRP2位于黑素细胞的黑色素体膜中，参与多巴色氨酸向5,6-二羟基吲哚的酶促转化，这是真黑素和浅黑素生成的关键步骤。这一过程不仅对皮肤、头发和眼睛的颜色至关重要，而且对抵御紫外线辐射也至关重要。TRP2的缺陷会导致色素沉着紊乱，凸显了其在维持正常色素沉着和整体皮肤健康方面的重要性。TRP2与其他酪氨酸酶相关蛋白（如TRP1和酪氨酸酶）相互作用，形成一种多酶复合物，从而提高黑色素的生成效率。在研究中使用抗TRP2抗体（C-9）可以深入了解色素沉着的机理以及控制黑色素合成的分子相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRP2/DCT 抗体 (C-9) 参考文献:

  1. trp2突变小鼠的性行为和社会行为发生改变。  |  Leypold, BG., et al. 2002. Proc Natl Acad Sci U S A. 99: 6376-81. PMID: 11972034
  2. 人类酪氨酸酶催化活性的突变图谱。  |  Tripathi, RK., et al. 1992. J Biol Chem. 267: 23707-12. PMID: 1429711
  3. 第二种与酪氨酸酶相关的蛋白质 TRP-2 是一种黑色素生成酶，被称为 DOPAchrome tautomerase。  |  Tsukamoto, K., et al. 1992. EMBO J. 11: 519-26. PMID: 1537333
  4. Trp2肽疫苗佐剂（R）-DOTAP抑制晚期黑色素瘤模型中的肿瘤生长。  |  Vasievich, EA., et al. 2012. Mol Pharm. 9: 261-8. PMID: 22142394
  5. 克隆和表达编码小鼠酪氨酸酶的 cDNA。  |  Shibahara, S., et al. 1986. Nucleic Acids Res. 14: 2413-27. PMID: 3008090
  6. 哺乳动物酪氨酸酶--黑色素细胞色素沉着的关键调节控制点。  |  Hearing, VJ. and Jiménez, M. 1987. Int J Biochem. 19: 1141-7. PMID: 3125075
  7. 具有本征自螯合 64Cu 特性的 Trp2 肽组装纳米粒子用于 PET 成像跟踪和基于树突状细胞的黑色素瘤免疫疗法。  |  He, Z., et al. 2021. ACS Appl Bio Mater. 4: 5707-5716. PMID: 35006752
  8. 苯硼酸酯修饰的聚合物纳米粒子在癌症免疫治疗中促进TRP2肽抗原递送。  |  Wang, Q., et al. 2022. Drug Deliv. 29: 2029-2043. PMID: 35766157
  9. 哺乳动物的酪氨酸酶催化黑色素生物合成过程中的三个反应。  |  Körner, A. and Pawelek, J. 1982. Science. 217: 1163-5. PMID: 6810464
  10. 高分子量形式的酪氨酸酶和酪氨酸酶相关蛋白：黑色素生成复合物的证据。  |  Orlow, SJ., et al. 1994. J Invest Dermatol. 103: 196-201. PMID: 8040609
  11. 人类酪氨酸酶相关蛋白-2 cDNA 的分子特征。黑色素细胞中的表达模式。  |  Bouchard, B., et al. 1994. Eur J Biochem. 219: 127-34. PMID: 8306979
  12. 黑色素生成复合物内的分子相互作用：来自 B16 小鼠黑色素瘤的酪氨酸酶和 TRP1 异二聚体的形成。  |  Jiménez-Cervantes, C., et al. 1998. Biochem Biophys Res Commun. 253: 761-7. PMID: 9918801

订购信息

产品名称产品编号规格价格数量收藏夹

TRP2/DCT 抗体 (C-9)

sc-74439
200 µg/ml
RMB2377.00

TRP2/DCT (C-9): m-IgG Fc BP-HRP 套装

sc-528665
200 µg Ab; 10 µg BP
RMB2662.00

TRP2/DCT (C-9): m-IgGκ BP-HRP 套装

sc-521112
200 µg Ab, 40 µg BP
RMB2662.00

TRP2/DCT (C-9): m-IgG2a BP-HRP 套装

sc-547137
200 µg Ab; 10 µg BP
RMB2662.00

TRP2/DCT 抗体 (C-9) AC

sc-74439 AC
500 µg/ml, 25% agarose
RMB3129.00

TRP2/DCT 抗体 (C-9) HRP

sc-74439 HRP
200 µg/ml
RMB2377.00

TRP2/DCT 抗体 (C-9) FITC

sc-74439 FITC
200 µg/ml
RMB2482.00

TRP2/DCT 抗体 (C-9) PE

sc-74439 PE
200 µg/ml
RMB2580.00

TRP2/DCT 抗体 (C-9) Alexa Fluor® 488

sc-74439 AF488
200 µg/ml
RMB2685.00

TRP2/DCT 抗体 (C-9) Alexa Fluor® 546

sc-74439 AF546
200 µg/ml
RMB2685.00

TRP2/DCT 抗体 (C-9) Alexa Fluor® 594

sc-74439 AF594
200 µg/ml
RMB2685.00

TRP2/DCT 抗体 (C-9) Alexa Fluor® 647

sc-74439 AF647
200 µg/ml
RMB2685.00

TRP2/DCT 抗体 (C-9) Alexa Fluor® 680

sc-74439 AF680
200 µg/ml
RMB2685.00

TRP2/DCT 抗体 (C-9) Alexa Fluor® 790

sc-74439 AF790
200 µg/ml
RMB2685.00

I am interested in TRP2/DCT Antibody (C-9): sc-74439 (FITC or PE conjugated). Do you offer a trial size or smaller aliquot for testing purposes?

Asked by: K221
Thank you for your question. At this time, we do not offer conjugated antibody samples. If you would like information on how to receive an unconjugated antibody sample please email [email protected]
Answered by: BlakeJ
Date published: 2025-04-01

We have bought TRP2/DCT Antibody (C-9) FITC. I want to stain the B16 melanoma cells with this antibody and measure with FACS. My question is whether I should stain the cells extracellularly(surface)or intracellularly(after fixation,permeabilization)?

Asked by: atrighods
Thank you for your question. TRP2/DCT Antibody (C-9): sc-74439 is specific against amino acid sequence 41-190 of TRP2 of human origin. This sequence corresponds to lumenal location within melanosomes and would require permeabilization for flow cytometry experiments.
Answered by: Tech Support Europe
Date published: 2024-11-07

Hi. Previously asked [2 years ago] noted above about whether or not the antibody works on canine tissue. Do you have any further information?

Asked by: AWVET
Thank you for your question. Based on our test results with this product Anti-TRP2/DCT Antibody (C-9) can only be recommended for the detection of TRP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. There are no publications citing use of this antibody with canine cells or tissue samples.
Answered by: Tech Support Europe
Date published: 2022-02-17

Hello, Does this Ab good for IF? In case it is, please point me to article\figure to appreciate its specificity.

Asked by: Ma_ya
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates! We are aware of the following product citations using this antibody for IF, these can be found on our website, please refer to PMID's: 30061114, 28095444 and 25324551.
Answered by: Technical Support
Date published: 2020-03-11

Has this clone been used in canine tissues?

Asked by: AWVET
Thank you for your question. This antibody has not yet been tested in canine samples and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-02-13

Can this antibody recognize the glycosylated form of TRP2?

Asked by: AbPolly
Thank you for your question. Yes, TRP2 (C-9) sc-74439 can detect both the precursor and glycosylated forms of TRP2.
Answered by: Technical Support 9
Date published: 2017-01-22
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Rated 5 out of 5 by from Very good signals in Western BlotProduces strong and clear bands in Western Blot analyses of melanoma cell lysates. (Used at 1:1000 dilutions)
Date published: 2023-11-30
Rated 5 out of 5 by from 功能很出色，条带很清晰，产品非常棒，品控非常好功能很出色，条带很清晰，产品非常棒，品控非常好功能很出色，条带很清晰，产品非常棒，品控非常好功能很出色，条带很清晰，产品非常棒，品控非常好功能很出色，条带很清晰，产品非常棒，品控非常好功能很出色，条带很清晰，产品非常棒，品控非常好功能很出色，条带很清晰，产品非常棒，品控非常好
Date published: 2023-10-07
Rated 5 out of 5 by from Great for western blotIt detects DCT in melanoma cells at expected molecular size between 70-90 kda
Date published: 2020-09-18
Rated 5 out of 5 by from very good antibodyIn our hands this antibody gives a very clean band by WB.
Date published: 2019-11-12
Rated 5 out of 5 by from Clean signalTRP2 (C-9) SC-74439 shows excellent specificity when used for Western blot in human MNT1 cell line.
Date published: 2018-04-17
Rated 5 out of 5 by from Great product !!Great western blot data; strong signal with low background; also very nice IHC staining
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of TRP2 expression in A-375 and Y79 whole cell lysates.
Date published: 2017-01-10
Rated 1 out of 5 by from Test von TRP2Antikörper ist leider nicht spezifisch! Negativkontrollen waren auch positiv für TRP2!
Date published: 2017-01-09
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TRP2/DCT 抗体 (C-9) is rated 4.4 out of 5 by 13.
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