Date published: 2025-9-7
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TrkB Antibody (F-1) - Immunofluorescence - Image 284678
Trk B 抗体 (F-1): sc-377218. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜和细胞质定位.
TrkB Antibody (F-1) - Immunofluorescence - Image 284678
Trk B 抗体 (F-1): sc-377218. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜和细胞质定位.
TrkB Antibody (F-1) - Western Blotting - Image 282555
Trk B 抗体 (F-1): sc-377218. 未转染: sc-117752 (A) 和转染人 Trk B: sc-113925 (B) 293T 全细胞裂解液中 Trk B 表达的 Western 印迹分析.
TrkB Antibody (F-1) - Immunohistochemistry - Image 293512
Trk B 抗体 (F-1): sc-377218. 福尔马林固定, 石蜡包埋的人海马组织的免疫过氧化物酶染色显示神经元细胞和胶质细胞的胞浆染色.
Trk B 抗体 (F-1): sc-377218. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜和细胞质定位.

TrkB 抗体 (F-1): sc-377218

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寫評論提問

说明书
  • TrkB抗体(F-1)是小鼠单克隆IgM κ, 在39篇文献中引用，规格为200 µg/ml
  • 抗原表位位于human来源Trk B胞外域的37-75氨基酸之间
  • 推荐用于 Trk B, Trk B-T1 and Trk B T-Shc of human origin; Trk B splice variants L1 and L10 of mouse origin; and Trk B gp95 and Trk B gp145 of rat origin WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Trk (B-3): sc-7268，可查看有标记的 TrkB 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取TrkB (F-1): sc-377218的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成TrkB 抗体 (F-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

额外Trk抗体，包括Trk A, Trk B和Trk C

TrkB抗体（F-1）是一种IgM κ小鼠单克隆TrkB抗体（也称为Trk B抗体或NTRK2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人源的TrkB、TrkB-T1和TrkB T-Shc；小鼠来源的TrkB剪接变体L1和L10；以及大鼠来源的TrkB gp95和TrkB gp145。Trk B抗体（F-1）可作为非偶联抗Trk B抗体形式提供。Trk原癌基因编码一种酪氨酸蛋白激酶，即Trk A，也称为Trk gp140，其作为某些神经营养因子的受体，包括神经生长因子（NGF）和神经营养素-3（NT-3）。Trk B是一种与Trk A高度相关的酪氨酸激酶基因。Trk B的表达仅限于中枢神经系统和外周神经系统内的组织。脑源性神经营养因子（BDNF）和NT-3，但不是NGF，可以诱导NTRK2编码的受体之一Trk B gp145的酪氨酸快速磷酸化，尽管BDNF引起的反应至少比NT-3大两个数量级。因此，似乎Trk B gp145可能代表BDNF和NT-3的神经营养受体。酪氨酸激酶Trk家族的第三个成员Trk C编码一种名为Trk C gp145的蛋白质，该蛋白质优先在脑组织中表达，与Trk A和Trk B同等相关，并且是NT-3的功能性受体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TrkB 抗体 (F-1) 参考文献:

  1. 体外神经元5-羟色胺表型的上调：BDNF和cAMP共享Trk B依赖性机制。  |  Rumajogee, P., et al. 2002. J Neurochem. 83: 1525-8. PMID: 12472905
  2. Trk-B信号通路的下调：重度抑郁症的可能发病机理。  |  Tsai, SJ. 2004. Med Hypotheses. 62: 215-8. PMID: 14962629
  3. trk酪氨酸蛋白激酶介导神经生长因子和神经营养素-3的有丝分裂特性。  |  Cordon-Cardo, C., et al. 1991. Cell. 66: 173-83. PMID: 1649007
  4. trkB 酪氨酸蛋白激酶是脑源性神经营养因子和神经营养素-3 的受体。  |  Klein, R., et al. 1991. Cell. 66: 395-403. PMID: 1649702
  5. trkC是酪氨酸蛋白激酶trk家族的新成员，是神经营养素-3的受体。  |  Lamballe, F., et al. 1991. Cell. 66: 967-79. PMID: 1653651
  6. NGF 诱导的 trk 原癌基因产物的酪氨酸磷酸化和酪氨酸激酶活性。  |  Kaplan, DR., et al. 1991. Nature. 350: 158-60. PMID: 1706478
  7. trk 原癌基因编码神经生长因子的受体。  |  Klein, R., et al. 1991. Cell. 65: 189-97. PMID: 1849459
  8. 高亲和力 NGF 结合需要 trk 原癌基因和低亲和力 NGF 受体的共同表达。  |  Hempstead, BL., et al. 1991. Nature. 350: 678-83. PMID: 1850821
  9. 脊髓损伤后，欧洲鳗鱼脑干脊髓再生模型中脑源性神经营养因子和trk B mRNA在大脑中的表达。  |  Dalton, VS., et al. 2009. Neurosci Lett. 461: 275-9. PMID: 19545610
  10. trkB 酪氨酸蛋白激酶基因编码缺乏催化激酶结构域的第二种神经源性受体。  |  Klein, R., et al. 1990. Cell. 61: 647-56. PMID: 2160854
  11. trkB，一种在小鼠神经发育过程中表达的新型酪氨酸蛋白激酶受体。  |  Klein, R., et al. 1989. EMBO J. 8: 3701-9. PMID: 2555172
  12. 7,8-二羟基黄酮治疗进行性 MPTP 帕金森病小鼠模型后，可恢复步态障碍和纹状体酪氨酸羟化酶/Trk-B 的丧失。  |  Massaquoi, MS., et al. 2020. Neuroscience. 433: 53-71. PMID: 32142862

订购信息

产品名称产品编号规格价格数量收藏夹

TrkB 抗体 (F-1)

sc-377218
200 µg/ml
RMB2377.00

TrkB (F-1) 中和勝肽

sc-377218 P
100 µg/0.5 ml
RMB511.00

Hi, it is indicated that this antibody binds to splice variants L1 and L10. What are these splice variants, from which study? Or is it meaning variants L1 and L0 from Ninkina et al, 1997?

Asked by: excelsior
Thank you for your question. TrkB Antibody (F-1): sc-377218 recognizes Trk B, Trk B-T1 and Trk B T-Shc of human origin; Trk B splice variants L1 and L10 of mouse origin; and Trk B gp95 and Trk B gp145 of rat origin by WB, IP, IF, IHC(P) and ELISA. Protein and splice variant sequences can be found on UniProt under protein accession numbers Q16620 (human) and P15209 (murine).
Answered by: Tech Support Europe
Date published: 2024-11-07

In the third image above of the immunoperoxidase staining of formalin fixed, paraffin-embedded hippocampus tissue showing cytoplasmic staining, I was wondering where to find the protocol and/or source of this staining as well as what 2° Ab was used.Thanks

Asked by: cszar
Thank you for your question. Please see attached our recommended protocol . We recommend using an anti-mouse IgM secondary . https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: BlakeJ
Date published: 2023-08-11

基本的な事で恐縮ですがお教えください。 一般的に１次抗体はIgGもしくはIgMですが、この抗体のようにIgMのメリットは何でしょうか？ 一般的な知識的では５量体であるがゆえに凝集したり、非特異的な反応はないのでしょうか？

Asked by: nanairo
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

请问这支抗体可以用于大鼠的WB、IHC、IF吗？

Asked by: Stephnia Wang
感谢您的提问。Trk B Antibody (F-1): sc-377218可以用于检测大鼠样本的Trk B gp95和Trk B gp145蛋白，可用于WB、IHC和IF实验。更多问题，请联系我们的技术支持部门：电话(+86) 021-60936351，邮箱[email protected]。 Thank you for your question. Trk B Antibody (F-1): sc-377218 recommended for detection of Trk B gp95 and Trk B gp145 of rat origin by WB, IP, IF, IHC(P) and ELISA. If you have any further questions, please contact our Technical Services department at (+86) 021-60936351 or [email protected].
Answered by: Technical Support 7
Date published: 2017-01-16

What is the recommended antigen retrieval method when working with paraffin-embedded sections in IHC?

Asked by: AbPolly
Thank you for your question. We recommend using sodium citrate buffer, pH 6, with heat for antigen retrieval. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-13

Can the blocking peptide sc-377218 P be used as a positive control in Western Blot?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended to be used as a negative control by preadsorbing the Trk B antibody sc-377218 with it prior to application. It is not appropriate for use as a positive control in Western Blot. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from Works great for WBWorks well for neonatal mouse lungs at the concentration of 30ug of protein. Ab dilution will be 1:300 for overnight at 4oC
Date published: 2024-12-13
Rated 5 out of 5 by from Great antibody!1:200 diluted for wb of rat heart protein,the band is strong and special
Date published: 2019-03-28
Rated 3 out of 5 by from 组化功能尚可配套和NT-4一起送的试用装，效果尚可，但是阳性率比较低，主要阳性出现的部位跟预实验结果有出入，准备继续重复
Date published: 2017-10-10
Rated 5 out of 5 by from Great antibody!I used it for Western blotting. It recognized Trk B successfully from the mouse brain samples.
Date published: 2017-09-27
Rated 3 out of 5 by from reviewIt does not work well even at 1:100.I don not find a wanted stripe on definite position.
Date published: 2017-09-20
Rated 5 out of 5 by from specific resultThe rat hippocampus was detected with this antibody, and the specificity was excellent. After 1:300 dilution, cytoplasm staining appeared , and sodium citrate antigen retrieval was recommended.
Date published: 2017-09-10
Rated 5 out of 5 by from 用此抗体做western,能做出来.用此抗体做western,能做出来.转膜条件200毫安,85分钟.免疫组化还没试,但愿都好用.哈哈哈哈哈
Date published: 2017-08-22
Rated 4 out of 5 by from Workable for IHCAntibody seems to work for IHC and IF staining in fixed songbird brain tissue but needs some optimization on our end. Not attempted for WB or flow.
Date published: 2017-06-09
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TrkB 抗体 (F-1) is rated 4.6 out of 5 by 16.
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