Date published: 2025-9-6
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Trimethyl Histone H3 Antibody (6F12-H4) - Western Blotting - Image 387587
使用三甲基组蛋白 H3 抗体 (6F12-H4) Alexa Fluor® 680: sc-130356 AF680 检测小鼠睾丸组织提取物中三甲基组蛋白 H3 的表达, 使用 GAPDH (G-9) Alexa Fluor® 790: sc-365062 AF790 检测小鼠睾丸组织提取物中 GAPDH 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Trimethyl Histone H3 Antibody (6F12-H4) - Western Blotting - Image 387587
使用三甲基组蛋白 H3 抗体 (6F12-H4) Alexa Fluor® 680: sc-130356 AF680 检测小鼠睾丸组织提取物中三甲基组蛋白 H3 的表达, 使用 GAPDH (G-9) Alexa Fluor® 790: sc-365062 AF790 检测小鼠睾丸组织提取物中 GAPDH 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
使用三甲基组蛋白 H3 抗体 (6F12-H4) Alexa Fluor® 680: sc-130356 AF680 检测小鼠睾丸组织提取物中三甲基组蛋白 H3 的表达, 使用 GAPDH (G-9) Alexa Fluor® 790: sc-365062 AF790 检测小鼠睾丸组织提取物中 GAPDH 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Trimethyl Histone H3 抗体 (6F12-H4): sc-130356

2.9(10)
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说明书
  • Trimethyl Histone H3 抗体 6F12-H4 是小鼠单克隆 IgG1, 在6篇文献中引用，提供 200 µg/ml
  • 针对含三甲基化Lysine 9（Lys 9 trimethylated Histone H3 of human起源）的短氨基酸序列
  • 抗-Trimethyl Histone H3 抗体 (6F12-H4) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 Lys 9 trimethylated Histone H3
  • 抗Trimethyl Histone H3抗体（6F12-H4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-130356 X, 200 µg/0.1 ml)
  • 关于如何获取Trimethyl Histone H3 (6F12-H4): sc-130356的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Trimethyl Histone H3 抗体 (6F12-H4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
说明书与实验方案
研究信息

関連項目

此外 Histone 抗体包括 Histone cluster 1 H1, Histone H2A, Histone H2B, Histone H3, Histone H4 和 Histone cluster 1 H3A

三甲基组蛋白H3抗体（6F12-H4）是一种IgG1型小鼠单克隆三甲基组蛋白H3抗体，可通过WB, IP和IF检测小鼠、大鼠和人类来源的赖氨酸9三甲基化组蛋白H3。三甲基组蛋白H3抗体（6F12-H4）可以以非偶联型抗三甲基组蛋白H3抗体形式提供，也可以以多种偶联形式的抗三甲基组蛋白H3抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。真核组蛋白是碱性的、水溶性的核蛋白，它们通过以左手超螺旋的方式依次包裹146个碱基对的DNA，形成异源八聚体核小体颗粒，进而构成染色质纤维。四个核心组蛋白（H2A、H2B、H3和H4）中的每一种都形成两个分子，构成八聚体；由两个H2A-H2B二聚体和两个H3-H4二聚体组成，通过三级结构形成两个近乎对称的部分。组蛋白主要受酶在其N端尾部的翻译后修饰，但也存在于其球状结构域中。人类组蛋白H3在赖氨酸9处受到三甲基化修饰，这种修饰对于特定的DNA交易或染色质状态转变可能是必要的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Trimethyl Histone H3 抗体 (6F12-H4) 参考文献:

  1. 转录抑制因子JHDM3A对三甲基组蛋白H3的赖氨酸9和赖氨酸36进行脱甲基化。  |  Klose, RJ., et al. 2006. Nature. 442: 312-6. PMID: 16732292
  2. 人类功能性中心粒的成核和维持需要最低限度的 CENP-A 核心。  |  Okamoto, Y., et al. 2007. EMBO J. 26: 1279-91. PMID: 17318187
  3. 组蛋白H3赖氨酸4的三甲基化水平普遍异常高，在很大程度上调节了"特权"基因的表达。  |  Chen, L., et al. 2007. Gene Expr. 13: 271-82. PMID: 17605300
  4. 甲基化组学：与staurosporine诱导的骨肉瘤细胞凋亡相关的组蛋白H3赖氨酸27的甲基化。  |  Cheng, MF., et al. 2009. Histol Histopathol. 24: 1105-11. PMID: 19609857
  5. DNA结合位点将NFATc1核定位到成人骨骼肌纤维中的异染色质区域。  |  Shen, T., et al. 2010. Histochem Cell Biol. 134: 387-402. PMID: 20865272
  6. GLT-1基因的CpG岛作为甲基化敏感增强子。  |  Perisic, T., et al. 2012. Glia. 60: 1345-55. PMID: 22593010
  7. 开发多种基于细胞的检测方法，用于检测组蛋白H3 Lys27三甲基化（H3K27me3）。  |  Qian, J., et al. 2013. Assay Drug Dev Technol. 11: 449-56. PMID: 23992119
  8. 肌醇焦磷酸调节JMJD2C依赖性组蛋白去甲基化。  |  Burton, A., et al. 2013. Proc Natl Acad Sci U S A. 110: 18970-5. PMID: 24191012
  9. SUV39H1/H3K9me3 可减轻磺草酮诱导的 PC3 前列腺癌细胞凋亡信号传导。  |  Watson, GW., et al. 2014. Oncogenesis. 3: e131. PMID: 25486523
  10. HMGB1介导的染色质重塑减弱了Il24基因的表达，从而保护免受过敏性接触性皮炎的影响。  |  Senda, N., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33443188

订购信息

产品名称产品编号规格价格数量收藏夹

Trimethyl Histone H3 抗体 (6F12-H4)

sc-130356
200 µg/ml
RMB2377.00

Trimethyl Histone H3 抗体 (6F12-H4) X

sc-130356 X
200 µg/0.1 ml
RMB2377.00

Trimethyl Histone H3 抗体 (6F12-H4) AC

sc-130356 AC
500 µg/ml, 25% agarose
RMB3129.00

Trimethyl Histone H3 抗体 (6F12-H4) HRP

sc-130356 HRP
200 µg/ml
RMB2377.00

Trimethyl Histone H3 抗体 (6F12-H4) FITC

sc-130356 FITC
200 µg/ml
RMB2482.00

Trimethyl Histone H3 抗体 (6F12-H4) PE

sc-130356 PE
200 µg/ml
RMB2580.00

Trimethyl Histone H3 抗体 (6F12-H4) Alexa Fluor® 488

sc-130356 AF488
200 µg/ml
RMB2685.00

Trimethyl Histone H3 抗体 (6F12-H4) Alexa Fluor® 546

sc-130356 AF546
200 µg/ml
RMB2685.00

Trimethyl Histone H3 抗体 (6F12-H4) Alexa Fluor® 594

sc-130356 AF594
200 µg/ml
RMB2685.00

Trimethyl Histone H3 抗体 (6F12-H4) Alexa Fluor® 647

sc-130356 AF647
200 µg/ml
RMB2685.00

Trimethyl Histone H3 抗体 (6F12-H4) Alexa Fluor® 680

sc-130356 AF680
200 µg/ml
RMB2685.00

Trimethyl Histone H3 抗体 (6F12-H4) Alexa Fluor® 790

sc-130356 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-130356 X be used instead of the standard form sc-130356?

Asked by: Randy McDonald
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Trimethyl Histone H3 Antibody (6F12-H4)This antibody didn't work at a dilution of 1:50 for immunofluorescent staining on the paraffin section from postnatal mouse lungs. I did antigen retrieval in citrate buffer and did not see any staining. We then ordered another similar antibody from another company. That antibody at dilution of 1:50 worked very well with the same paraffin section. The results at least confirmed our paraffin section and the staining method had no problem.
Date published: 2023-03-06
Rated 5 out of 5 by from Good work!This antibody gave a strong and clean signal when we used it at a 1:200 diluition with 40μg of protein samples.
Date published: 2022-05-26
Rated 2 out of 5 by from Buono per WB, non funziona in IFQuesto anticorpo funziona molto bene in Western Blot, ha una buona resa, pulita da bande aspecifiche. Invece per quanto riguarda l' applicazione in IF non sono riuscita a vedere alcun segnale né in immunoistochimica applicata a microscopia a fluorescenza, né in immunoistochimica applicata a microscopia elettronica a trasmissione.
Date published: 2020-11-13
Rated 1 out of 5 by from Not working on IFI tried the antibody at 1/50 and 1/200 concentrations in an immunostaining of mouse brain tissue with previous antigen retrieval (citrate buffer) and I did not see any staining
Date published: 2018-01-15
Rated 1 out of 5 by from immunofluoresenceFor IF assay I test it with Hela cells and bone amrrow rat MSC. It does not work at any concentration.
Date published: 2017-12-15
Rated 2 out of 5 by from Did Not Work On WesternUsed this antibody for a western at concentrations of 1:1000, 1:500, and 1:250 and trimethyl histone H3 did not show up in control or experimental group.
Date published: 2017-09-19
Rated 4 out of 5 by from a relative good antibody for WBThis antibody generates a signal at the predicted position on the western blot (1:500 dilution, 40ug HeLa extract). It does not stain the nucleus (1:200, formaldehyde-fixed HeLa)
Date published: 2017-09-01
Rated 5 out of 5 by from want to get the coupon'Receiving 15 Cruz Credits'I bought one from cell signaling, but it didn't work and I hope to get some sample to try.
Date published: 2017-02-02
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Trimethyl Histone H3 抗体 (6F12-H4) is rated 2.9 out of 5 by 10.
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