Date published: 2025-9-7

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Trichohyalin 抗体 (E-11): sc-376684

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说明书
  • Trichohyalin 抗体 E-11 是小鼠单克隆 IgG1 (kappa轻链),Trichohyalin抗体,规格为200 µg/ml
  • 免疫原氨基酸序列23-215位于human物种的Trichohyalin的N-terminus附近
  • Trichohyalin 抗体 (E-11) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 Trichohyalin
  • 抗Trichohyalin抗体(E-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Trichohyalin (E-11): sc-376684免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Trichohyalin Antibody (E-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Trichohyalin Antibody (E-11) 打包提供(请参阅下面的订购信息)。

    快捷链接

    関連項目

    Trichohyalin抗体(E-11)是一种IgG1 κ小鼠单克隆Trichohyalin抗体(也称为Trichohyalin抗体),可通过WB、IP、IF和ELISA检测人源Trichohyalin蛋白。Trichohyalin抗体(E-11)既可作为非偶联抗Trichohyalin抗体形式提供,也可作为多种偶联形式的抗Trichohyalin抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Trichohyalin是一种含有九个结构域的结构蛋白,在毛囊的髓质和内根鞘中产生。其中结构基序包括位于结构域1中的两个ERF-hand钙结合域。它是S100融合蛋白家族的成员,也是转谷氨酰胺酶和肽基精氨酸脱亚氨酶的底物。Trichohyalin与角蛋白中间丝(KIF)和周边细胞包膜屏障蛋白结合,以协调角化细胞包膜的组织。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Trichohyalin 抗体 (E-11) 参考文献:

    1. 毛鳞蛋白在皮肤病中的表达:通过免疫组织化学染色与角质蛋白和丝聚蛋白的比较研究。  |  Lee, SC., et al. 1999. Acta Derm Venereol. 79: 122-6. PMID: 10228630
    2. 毛鳞蛋白在皮肤病中的表达。  |  Manabe, M. and Ogawa, H. 1999. Exp Dermatol. 8: 312. PMID: 10439242
    3. 角蛋白、毛蛋白和角蛋白-毛蛋白混合颗粒:概述。  |  Manabe, M. and O'Guin, WM. 1992. J Dermatol. 19: 749-55. PMID: 1284067
    4. 毛鳞蛋白:存在于正常人表皮颗粒层和角质层中。  |  Hamilton, EH., et al. 1991. J Invest Dermatol. 96: 666-72. PMID: 1708794
    5. 毛萼蛋白与中间丝的相互作用:毛萼蛋白成熟的三个免疫学定义阶段。  |  O'Guin, WM., et al. 1992. J Invest Dermatol. 98: 24-32. PMID: 1728637
    6. 毛鳞蛋白是人类斑秃的潜在主要自身抗原。  |  Leung, MC., et al. 2010. J Proteome Res. 9: 5153-63. PMID: 20722389
    7. 鉴定出一种在毛囊中选择性表达的s100融合型蛋白--类毛鳞蛋白1。  |  Wu, Z., et al. 2011. J Invest Dermatol. 131: 1761-3. PMID: 21614011
    8. 类毛鳞蛋白样蛋白在皮肤附属物的形态发生中具有进化保守的作用。  |  Mlitz, V., et al. 2014. J Invest Dermatol. 134: 2685-2692. PMID: 24780931
    9. 毛萼蛋白-角萼蛋白杂交颗粒的存在:非叶状上皮中两种主要中间丝相关蛋白的共定位。  |  Manabe, M. and O'Guin, WM. 1994. Differentiation. 58: 65-75. PMID: 7532602
    10. 毛鳞蛋白:头发、舌头、指甲和表皮的一种结构蛋白。  |  O'Keefe, EJ., et al. 1993. J Invest Dermatol. 101: 65S-71S. PMID: 7686953

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Trichohyalin 抗体 (E-11)

    sc-376684
    200 µg/ml
    RMB2377.00

    Trichohyalin (E-11): m-IgG Fc BP-HRP 套装

    sc-529922
    200 µg Ab; 10 µg BP
    RMB2662.00

    Trichohyalin (E-11): m-IgGκ BP-HRP 套装

    sc-523045
    200 µg Ab, 40 µg BP
    RMB2662.00

    Trichohyalin (E-11): m-IgG1 BP-HRP 套装

    sc-543767
    200 µg Ab; 20 µg BP
    RMB2662.00

    Trichohyalin 抗体 (E-11) AC

    sc-376684 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    Trichohyalin 抗体 (E-11) HRP

    sc-376684 HRP
    200 µg/ml
    RMB2377.00

    Trichohyalin 抗体 (E-11) FITC

    sc-376684 FITC
    200 µg/ml
    RMB2482.00

    Trichohyalin 抗体 (E-11) PE

    sc-376684 PE
    200 µg/ml
    RMB2580.00

    Trichohyalin 抗体 (E-11) Alexa Fluor® 488

    sc-376684 AF488
    200 µg/ml
    RMB2685.00

    Trichohyalin 抗体 (E-11) Alexa Fluor® 546

    sc-376684 AF546
    200 µg/ml
    RMB2685.00

    Trichohyalin 抗体 (E-11) Alexa Fluor® 594

    sc-376684 AF594
    200 µg/ml
    RMB2685.00

    Trichohyalin 抗体 (E-11) Alexa Fluor® 647

    sc-376684 AF647
    200 µg/ml
    RMB2685.00

    Trichohyalin 抗体 (E-11) Alexa Fluor® 680

    sc-376684 AF680
    200 µg/ml
    RMB2685.00

    Trichohyalin 抗体 (E-11) Alexa Fluor® 790

    sc-376684 AF790
    200 µg/ml
    RMB2685.00

    Hi. I bought Trichohyalin Antibody (F-2): sc-515130. I need a protocol and kind a sample like positive control for wester blot. Can yuo send me an extract to probe this antibody please. I agree in advance. Thank you.

    Asked by: Francisco Sandoval
    Thank you for your question. A link to our recommended protocol for Western blotting can be found below. We recommend using HeLa (sc-2200), A-431 (sc-2201) and SW480 (sc-2219) whole cell lysates as positive controls for Western blotting with this antibody. We do not provide sample sizes of our whole cell lysates, but they can be purchased as positive controls. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2019-01-26

    What is the recommended starting dilution for Western blot for sc-376684: Trichohyalin (E-11) monoclonal antibody?

    Asked by: Germaine
    The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-22
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    Rated 5 out of 5 by from Produced positive Western Blot data of TrichohyalinProduced positive Western Blot data of Trichohyalin expression in CCD-1064Sk whole cell lysate and human lung tissue extract . -SCBT QC
    Date published: 2015-04-22
    Rated 5 out of 5 by from Great Western Blot data of Trichohyalin expressionGreat Western Blot data of Trichohyalin expression in Hs 732. -SCBT QC
    Date published: 2014-04-02
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    Trichohyalin 抗体 (E-11) is rated 5.0 out of 5 by 2.
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