Date published: 2025-9-7

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TRF1 抗体 (G-7): sc-271485

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说明书
  • TRF1 抗体 G-7 是小鼠单克隆 IgG1 κ,TRF1抗体, 在4篇文献中引用,规格为200 µg/ml
  • 针对human起源的TRF1内部区域内的氨基酸155-396进行
  • TRF1 抗体 (G-7) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 TRF1
  • 抗TRF1抗体(G-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRF1 (G-7): sc-271485免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是TRF1 Antibody (G-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与TRF1 Antibody (G-7) 搭配使用(请参阅下面的订购信息)。

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関連項目

TRF1抗体(G-7)是一种IgG1κ小鼠单克隆TRF1抗体(也称为端粒重复结合因子1抗体、PIN2抗体、TRF抗体、端粒重复结合因子(NIMA相互作用)1抗体、TTAGGG重复结合因子1抗体、端粒蛋白Pin2/TRF1抗体、NIMA相互作用蛋白2抗体或TRBF1抗体),可通过WB、IP、IF和ELISA检测人源TRF1蛋白。 TRF1抗体(G-7)可作为非偶联抗TRF1抗体形式,以及多种偶联形式的抗TRF1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。端粒重复结合因子1(TERF1、PIN2、TRF1、TRBF1)和2(TERF2、TRF2、TRBF2)在整个细胞周期中存在于端粒,它们通过顺式作用限制单个染色体末端的延长来调节端粒酶。端粒酶将5'-TTAGGG-3'的六聚重复序列添加到染色体DNA的末端。这种端粒酶在细胞永生化与细胞衰老中起着重要作用。 TRF1负调控端粒延长,而TRF2通过抑制末端融合来保护染色体末端。肿瘤细胞中TRF表达的下调可能导致细胞永生化与恶性进展。 TRF1具有酸性N末端,而TRF2具有碱性N末端。 TRF2在G0和S期定位于核仁,在G2期扩散出核仁。在有丝分裂期间,TRF2从浓缩的染色体上分散,在细胞分裂时回到核仁。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRF1 抗体 (G-7) 参考文献:

  1. Pin2/TRF1在端粒维持和细胞周期控制中的作用。  |  Zhou, XZ., et al. 2003. J Cell Biochem. 89: 19-37. PMID: 12682905
  2. TRF1和TRF2的翻译后修饰及其在端粒维持中的作用。  |  Walker, JR. and Zhu, XD. 2012. Mech Ageing Dev. 133: 421-34. PMID: 22634377
  3. 剪接因子U2AF65通过抑制泛素依赖性蛋白水解来稳定TRF1蛋白。  |  Kim, J. and Chung, IK. 2014. Biochem Biophys Res Commun. 443: 1124-30. PMID: 24389012
  4. TRF1组织单个人类端粒的动态。  |  Li, X., et al. 2021. Nucleic Acids Res. 49: 760-775. PMID: 33347580
  5. TRF1-TIN2介导的端粒DNA反式和顺式相互作用的结构, 动力学和调控。  |  Pan, H., et al. 2021. J Biol Chem. 297: 101080. PMID: 34403696
  6. TRF1消耗揭示了小鼠卵母细胞中端粒, 着丝点和内中心粒之间的相互调控。  |  Jeon, HJ. and Oh, JS. 2021. Front Cell Dev Biol. 9: 749116. PMID: 34604243
  7. TRF1、TIN2和SA1对端粒DNA压缩的组装路径依赖性。  |  Liu, M., et al. 2023. Biophys J. 122: 1822-1832. PMID: 37081787
  8. 端粒核小体识别结构基础。  |  Hu, H., et al. 2023. Sci Adv. 9: eadi4148. PMID: 37624885
  9. TRF1和TRF2在非小细胞肺癌中的不同作用。  |  Chae, M., et al. 2024. Onco Targets Ther. 17: 463-469. PMID: 38855632
  10. TRF1是一种二聚体,可弯曲端粒DNA。  |  Bianchi, A., et al. 1997. EMBO J. 16: 1785-94. PMID: 9130722

订购信息

产品名称产品编号规格价格数量收藏夹

TRF1 抗体 (G-7)

sc-271485
200 µg/ml
RMB2377.00

TRF1 (G-7): m-IgG Fc BP-HRP 套装

sc-525810
200 µg Ab; 10 µg BP
RMB2662.00

TRF1 抗体 (G-7) AC

sc-271485 AC
500 µg/ml, 25% agarose
RMB3129.00

TRF1 抗体 (G-7) HRP

sc-271485 HRP
200 µg/ml
RMB2377.00

TRF1 抗体 (G-7) FITC

sc-271485 FITC
200 µg/ml
RMB2482.00

TRF1 抗体 (G-7) PE

sc-271485 PE
200 µg/ml
RMB2580.00

TRF1 抗体 (G-7) Alexa Fluor® 488

sc-271485 AF488
200 µg/ml
RMB2685.00

TRF1 抗体 (G-7) Alexa Fluor® 546

sc-271485 AF546
200 µg/ml
RMB2685.00

TRF1 抗体 (G-7) Alexa Fluor® 594

sc-271485 AF594
200 µg/ml
RMB2685.00

TRF1 抗体 (G-7) Alexa Fluor® 647

sc-271485 AF647
200 µg/ml
RMB2685.00

TRF1 抗体 (G-7) Alexa Fluor® 680

sc-271485 AF680
200 µg/ml
RMB2685.00

TRF1 抗体 (G-7) Alexa Fluor® 790

sc-271485 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for sc-271485: TRF1 (G-7) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Sigma 1 Receptor antibodySigma 1 Receptor antibody was obtained from SCBT Inc (Cat # sc-166392; mouse monoclonal). Excellent antibody for IF Published in Journal of Neuroimmune Pharmacology https://doi.org/10.1007/s11481-018-9787-4
Date published: 2018-04-12
Rated 1 out of 5 by from Not suitable for IHC(P)We tested this antibody along with another TRF1 clone in human endometrial tissue. No specific staining was observed after overnight incubation at 1:50 following citrate buffer (pH6) antigen retrieval.
Date published: 2017-11-14
Rated 4 out of 5 by from No non- specific band but does not match MWSignal was good and specific but molecular weight is in between 43-56 KDA in esophageal cancer cell
Date published: 2017-11-13
Rated 3 out of 5 by from it is goodI diluted this antibody wtih 1:250, but this molecular weight is 130KD.
Date published: 2017-08-29
Rated 5 out of 5 by from Works for WB/IF/PLABought this antibody (TRF1) to measure protein-protein interactions in 293T/HeLa lysates. Worked great!
Date published: 2017-08-15
Rated 5 out of 5 by from WBThis antibody worked well for me with 1:200 and ECL prime
Date published: 2017-05-30
Rated 5 out of 5 by from Produced nice Western blot data of TRF1 expressionProduced nice Western blot data of TRF1 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2014-08-11
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TRF1 抗体 (G-7) is rated 4.0 out of 5 by 7.
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