Date published: 2025-9-9
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transferrin Antibody (F-8) - Western Blotting - Image 147891
转铁蛋白抗体 (F-8): sc-373785. 转铁蛋白在 c4 全细胞裂解液中表达的 Western 印迹分析.
转铁蛋白抗体 (F-8): sc-373785. 转铁蛋白在 c4 全细胞裂解液中表达的 Western 印迹分析.

transferrin 抗体 (F-8): sc-373785

4.5(11)
寫評論提問

说明书
  • transferrin抗体(F-8)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的transferrin的内部区域内的氨基酸420-453之间
  • transferrin 抗体 (F-8) 推荐用于WB, IP, IF 和 ELISA，检测mouse 来源的 transferrin
  • 抗transferrin抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取transferrin (F-8): sc-373785的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是transferrin Antibody (F-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与transferrin Antibody (F-8) 打包提供（请参阅下面的订购信息）。

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基因信息
说明书与实验方案
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関連項目

Transferrin Antibody (F-8) 是一种 IgG1 κ 小鼠单克隆转铁蛋白抗体（也称为转铁蛋白抗体或 TF（转铁蛋白）抗体），可通过 WB、IP、IF 和 ELISA 检测小鼠来源的转铁蛋白。Transferrin Antibody (F-8) 既可以是非偶联的抗转铁蛋白抗体形式，也可以是多种偶联形式的抗转铁蛋白抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。铁（Fe）是一种在所有活细胞中都存在的严格代谢控制的矿物质和生长因子。未与红细胞血红蛋白结合的铁由转铁蛋白（Tf）转运，转铁蛋白是脊椎动物血清中的铁转运蛋白。转铁蛋白包含两个同源结构域，每个结构域都包含一个 Fe 结合位点。大多数转铁蛋白在肝脏中合成并分泌到血液中，但也在睾丸和大脑以及少突胶质细胞中少量产生，其中转铁蛋白是少突胶质细胞分化的早期标志物。转铁蛋白在与转铁蛋白受体（TfR）（也称为 CD71）结合后，通过被覆凹坑和囊泡系统被红细胞和网织红细胞内化。释放 Fe 后，转铁蛋白返回细胞外介质，在那里它可以被重复使用。转铁蛋白基因的缺陷导致无转铁蛋白血症，这是一种罕见的常染色体隐性遗传病，其特征是小细胞性贫血和铁负荷。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

transferrin 抗体 (F-8)

sc-373785
200 µg/ml
RMB2377.00

transferrin (F-8): m-IgG Fc BP-HRP 套装

sc-529563
200 µg Ab; 10 µg BP
RMB2662.00

transferrin (F-8): m-IgGκ BP-HRP 套装

sc-522499
200 µg Ab, 40 µg BP
RMB2662.00

transferrin (F-8): m-IgG1 BP-HRP 套装

sc-543581
200 µg Ab; 20 µg BP
RMB2662.00

transferrin 抗体 (F-8) AC

sc-373785 AC
500 µg/ml, 25% agarose
RMB3129.00

transferrin 抗体 (F-8) HRP

sc-373785 HRP
200 µg/ml
RMB2377.00

transferrin 抗体 (F-8) FITC

sc-373785 FITC
200 µg/ml
RMB2482.00

transferrin 抗体 (F-8) PE

sc-373785 PE
200 µg/ml
RMB2580.00

transferrin 抗体 (F-8) Alexa Fluor® 488

sc-373785 AF488
200 µg/ml
RMB2685.00

transferrin 抗体 (F-8) Alexa Fluor® 546

sc-373785 AF546
200 µg/ml
RMB2685.00

transferrin 抗体 (F-8) Alexa Fluor® 594

sc-373785 AF594
200 µg/ml
RMB2685.00

transferrin 抗体 (F-8) Alexa Fluor® 647

sc-373785 AF647
200 µg/ml
RMB2685.00

transferrin 抗体 (F-8) Alexa Fluor® 680

sc-373785 AF680
200 µg/ml
RMB2685.00

transferrin 抗体 (F-8) Alexa Fluor® 790

sc-373785 AF790
200 µg/ml
RMB2685.00

transferrin (F-8) 中和勝肽

sc-373785 P
100 µg/0.5 ml
RMB511.00

How can this mouse monoclonal transferrin antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, transferrin (F-8), is currently available conjugated to agarose (sc-373785 AC), HRP (sc-373785 HRP), phycoerythrin (sc-373785 PE), fluorescein (sc-373785 FITC), Alexa Fluor® 488 (sc-373785 AF488) and Alexa Fluor® 647 (sc-373785 AF647).
Answered by: Technical Support 7
Date published: 2017-02-08
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Rated 4 out of 5 by from Good for western blot, not for PLAThis is one of my two go-to Tf antibodies for western blotting. Works great! I tried to use this antibody for proximity ligation assay (PLA) and it did not work.
Date published: 2022-04-05
Rated 2 out of 5 by from WBtransferrin Antibody (F-8) did not worked in the WB of mouse lung primary fibroblast. We used anti-mouse IgG1 as the secondary antibody.
Date published: 2018-03-28
Rated 4 out of 5 by from Great.The band is quite clear. It is a good loading control for serum samples.
Date published: 2017-11-28
Rated 5 out of 5 by from Extraordinarily good antibody!This anti-Tf antibody is wonderful, one of the best antibodies that I used from SantaCruz. The antibody clearly recognized two bands at around 95 kda in human liver tissue. Liver is known for manufacturing Tf. The specificity of the antibody is further proven by the fact that it does not show immuno-reactivity in muscle tissue which does not express Tf (see the blot). Tf is known to be highly phosphorylated, glycosylated and palmitoylated, which may explain additional mass shift. Non-specific faint band is visible sometimes at around 55kda. Excellent antibody, very happy with this product and highly recommend it for your research. Good luck.
Date published: 2017-11-14
Rated 5 out of 5 by from 可以我做的大鼠样品，可以跑出一个明晰的条带，在80kd左右，分子量是对的，特异性也挺好，值得推广及购买！
Date published: 2017-10-17
Rated 4 out of 5 by from quiet good!It is better than others in fact. Good enough! it is suitable for your WB!
Date published: 2017-07-31
Rated 5 out of 5 by from The preferred choice for mouse!This mouse monoclonal transferrin Antibody (F-8) is the preferred choice for detection in mouse. Clean Western blot data in C4 lysates.
Date published: 2017-01-31
Rated 5 out of 5 by from We tried this antibody in WB and worked greatWe tried this antibody in WB and worked great, clean band at 1:500 dilution.
Date published: 2015-09-02
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transferrin 抗体 (F-8) is rated 4.5 out of 5 by 11.
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