Date published: 2025-9-6
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TRAIL Antibody (D-3) - Western Blotting - Image 79
TRAIL 抗体 (D-3): sc-8440. 人重组 TRAIL 融合蛋白的 Western 印迹分析.
TRAIL Antibody (D-3) - Western Blotting - Image 79
TRAIL 抗体 (D-3): sc-8440. 人重组 TRAIL 融合蛋白的 Western 印迹分析.
TRAIL 抗体 (D-3): sc-8440. 人重组 TRAIL 融合蛋白的 Western 印迹分析.

TRAIL 抗体 (D-3): sc-8440

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寫評論提問

说明书
  • TRAIL 抗体 D-3 是小鼠单克隆 IgG1 κ，TRAIL抗体, 在14篇文献中引用，规格为200 µg/ml
  • 免疫human来源TRAIL位于C-terminus 的25-281氨基酸
  • TRAIL 抗体 (D-3) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 TRAIL
  • 抗TRAIL抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRAIL (D-3): sc-8440的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是TRAIL Antibody (D-3) for WB applications. 的首选辅助检测试剂，这些试剂现与TRAIL Antibody (D-3) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

TRAIL抗体（D-3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测人类TRAIL蛋白。TRAIL (D-3)抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。TRAIL，也称为TNF相关凋亡诱导配体，在细胞凋亡调节中起着至关重要的作用，而细胞凋亡对于维持细胞稳态和免疫系统功能至关重要。TRAIL能够通过与特定受体结合来触发癌细胞的程序性细胞死亡，而不会影响正常细胞，因此成为癌症治疗研究的重要焦点。TRAIL能够选择性诱导细胞凋亡，这凸显了TRAIL作为治疗药物的潜力，因为它可以帮助消除恶性细胞，而不会对健康组织造成不利影响。TRAIL属于肿瘤坏死因子（TNF）超家族，该家族还包括其他重要的细胞因子，它们能够调节炎症和免疫反应。TRAIL在各种组织中都有表达，而TNF家族中的一些成员则主要在活化的T细胞中表达，这凸显了TRAIL在正常生理和病理条件下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRAIL 抗体 (D-3) 参考文献:

  1. TRAIL（Apo2L）可抑制原发性人类白血病和骨髓增生异常原的生长。  |  Plasilova, M., et al. 2002. Leukemia. 16: 67-73. PMID: 11840265
  2. 合成三萜类化合物与肿瘤坏死因子相关凋亡诱导配体合作诱导乳腺癌细胞凋亡。  |  Hyer, ML., et al. 2005. Cancer Res. 65: 4799-808. PMID: 15930300
  3. 糖基化对 TNF 相关凋亡诱导配体信号的调控  |  Micheau, O. 2018. Int J Mol Sci. 19: PMID: 29498673
  4. 强直性脊柱炎患者的 TNF 相关凋亡诱导配体受体 1  |  Karadag, DT., et al. 2020. J Clin Rheumatol. 26: 242-247. PMID: 31094932
  5. TNF 相关凋亡诱导配体在 1 型糖尿病中的免疫调节功能  |  Fogarasi, M. and Dima, S. 2024. Cells. 13: PMID: 39451194
  6. TNF 受体超家族的配体家族。  |  Cosman, D. 1994. Stem Cells. 12: 440-55. PMID: 7528588
  7. 法斯死亡因素  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
  8. FasL/TNF 死亡信号的竞争者。  |  Cleveland, JL. and Ihle, JN. 1995. Cell. 81: 479-82. PMID: 7758103
  9. 细胞和病毒蛋白 TNF 受体超家族：激活, 成本刺激和死亡。  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429
  10. 生与死的转换器TNF 受体超家族及相关蛋白。  |  Baker, SJ. and Reddy, EP. 1996. Oncogene. 12: 1-9. PMID: 8552378
  11. 肿瘤坏死因子细胞因子家族的新成员 Apo-2 配体诱导细胞凋亡。  |  Pitti, RM., et al. 1996. J Biol Chem. 271: 12687-90. PMID: 8663110
  12. 鉴定 TNF 家族中可诱导细胞凋亡的新成员并确定其特征。  |  Wiley, SR., et al. 1995. Immunity. 3: 673-82. PMID: 8777713

订购信息

产品名称产品编号规格价格数量收藏夹

TRAIL 抗体 (D-3)

sc-8440
200 µg/ml
RMB2377.00

TRAIL (D-3): m-IgG Fc BP-HRP 套装

sc-526526
200 µg Ab; 10 µg BP
RMB2662.00

TRAIL (D-3): m-IgG1 BP-HRP 套装

sc-531899
200 µg Ab; 20 µg BP
RMB2662.00

TRAIL 抗体 (D-3) AC

sc-8440 AC
500 µg/ml, 25% agarose
RMB3129.00

TRAIL 抗体 (D-3) HRP

sc-8440 HRP
200 µg/ml
RMB2377.00

TRAIL 抗体 (D-3) FITC

sc-8440 FITC
200 µg/ml
RMB2482.00

TRAIL 抗体 (D-3) PE

sc-8440 PE
200 µg/ml
RMB2580.00

TRAIL 抗体 (D-3) Alexa Fluor® 488

sc-8440 AF488
200 µg/ml
RMB2685.00

TRAIL 抗体 (D-3) Alexa Fluor® 546

sc-8440 AF546
200 µg/ml
RMB2685.00

TRAIL 抗体 (D-3) Alexa Fluor® 594

sc-8440 AF594
200 µg/ml
RMB2685.00

TRAIL 抗体 (D-3) Alexa Fluor® 647

sc-8440 AF647
200 µg/ml
RMB2685.00

TRAIL 抗体 (D-3) Alexa Fluor® 680

sc-8440 AF680
200 µg/ml
RMB2685.00

TRAIL 抗体 (D-3) Alexa Fluor® 790

sc-8440 AF790
200 µg/ml
RMB2685.00

Hi, Can I get a free sample of 10 µg sample of sc-8440 AF680. Thanks, Nidhi

Asked by: Nids
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 µg sample of our well characterized monoclonal antibody.
Answered by: Tech Service
Date published: 2018-11-30

Any information concerning assaying the levels of the various isoforms of TRAIL in blood?

Asked by: Steve1
Thank you for your question. Unfortunately this is not something we have any information about as we do not perform this type of assay. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-15

What is the recommended starting dilution for Western blot for sc-8440: TRAIL (D-3) monoclonal antibody?

Asked by: Dr Ninau Qelp
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good FeaturesTRAIL Antibody (D-3) is useful for protein imprinting in chicken cardiac muscle cells, and the company's antibody is very good.
Date published: 2017-11-09
Rated 4 out of 5 by from WB resultsspecific but not strong band was showed in WB in protein lysates of U2OS cells (1:300).
Date published: 2017-09-17
Rated 5 out of 5 by from Much improved TRAIL antibodyCompared to anti TRAIL H-257, this one is much cleaner!
Date published: 2017-03-14
Rated 5 out of 5 by from good stuffUsing this antibody to publish a paper. The data is very promising.
Date published: 2017-03-13
Rated 5 out of 5 by from Published data for WB in human neutrophilsPublished data for WB in human neutrophils and PBMCs. -SCBT Publication Review
Date published: 2015-03-08
Rated 5 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in WB using LNCaP and PC3 -SCBT Publication Review
Date published: 2015-03-08
Rated 5 out of 5 by from Published data for WB in human wildPublished data for WB in human wild-type and transfected A549 cells. -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for WB on canine chondrocyte - SCBT Publication Review
Date published: 2015-02-23
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TRAIL 抗体 (D-3) is rated 4.9 out of 5 by 9.
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