Date published: 2025-9-7
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TRAF6 Antibody (D-10) - Immunohistochemistry - Image 351541
TRAF6 抗体 (D-10): sc-8409. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色显示肾小管细胞的胞浆染色.
TRAF6 抗体 (D-10): sc-8409. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色显示肾小管细胞的胞浆染色.

TRAF6 抗体 (D-10): sc-8409

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说明书
  • TRAF6 抗体 D-10 是小鼠单克隆 IgG1 κ，TRAF6抗体, 在244篇文献中引用，规格为200 µg/ml
  • 免疫human来源TRAF6位于N-terminus 的1-274氨基酸
  • TRAF6 抗体 (D-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TRAF6
  • 抗TRAF6抗体（D-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TRAF6 (D-10): sc-8409的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 TRAF6 抗体 (D-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TRAF6 抗体 (D-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TRAF 抗体 包括 TRAF1, TRAF2, TRAF3, TRAF4, TRAF5和TRAF6

TRAF6抗体（D-10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的TRAF6蛋白。TRAF6 (D-10) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。肿瘤坏死因子受体相关因子6（TRAF6）在调节适应性免疫、先天免疫和骨代谢方面发挥着关键作用，因此TRAF6对于维持免疫平衡和骨骼完整性至关重要。作为泛素连接酶，TRAF6通过形成独特的泛素链（通过泛素赖氨酸63位连接）来激活关键蛋白激酶，如转化生长因子β激活的激酶（TAK1）和IκB激酶（IKK）。这种翻译后修饰对于激活NFκB信号通路以响应白细胞介素-1和Toll样受体配体至关重要，突出了TRAF6在免疫信号传导中的重要性。TRAF6的螺旋-螺旋结构域对于TRAF6的自身泛素化和随后NFκB通路的激活尤为重要。此外，TRAF6与多种蛋白激酶相互作用，包括IRAK1/IRAK、SRC和PKCΩ，在不同的信号通路之间建立联系，这些信号通路对于细胞对压力和炎症的反应至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRAF6 抗体 (D-10) 参考文献:

  1. TRAF6 与 MAST205 的相互作用可调节 NF-kappaB 的激活和 MAST205 的稳定性。  |  Xiong, H., et al. 2004. J Biol Chem. 279: 43675-83. PMID: 15308666
  2. TRAF6 的盘绕线圈结构域对其自动泛素化至关重要。  |  Yang, K., et al. 2004. Biochem Biophys Res Commun. 324: 432-9. PMID: 15465037
  3. TIFA 通过促进 TRAF6 的寡聚化和泛素化来激活 IkappaB 激酶（IKK）。  |  Ea, CK., et al. 2004. Proc Natl Acad Sci U S A. 101: 15318-23. PMID: 15492226
  4. TRAF6 在信号转导和免疫反应中的作用。  |  Kobayashi, T., et al. 2004. Microbes Infect. 6: 1333-8. PMID: 15555541
  5. 白细胞介素-8 通过 TRAF6 依赖性途径诱导核转录因子-kappaB。  |  Manna, SK. and Ramesh, GT. 2005. J Biol Chem. 280: 7010-21. PMID: 15591054
  6. RANK 介导的 TRAF6 信号放大导致破骨细胞生成过程中的 NFATc1 诱导。  |  Gohda, J., et al. 2005. EMBO J. 24: 790-9. PMID: 15678102
  7. TRAF6 信号的强度决定破骨细胞的生成。  |  Kadono, Y., et al. 2005. EMBO Rep. 6: 171-6. PMID: 15678156
  8. 促动脉粥样硬化细胞因子白细胞介素-18通过MyD88, 白细胞介素-1受体相关激酶, 肿瘤坏死因子受体相关因子6, c-Src, 磷脂酰肌醇3-激酶, Akt, c-Jun N-末端激酶和激活蛋白-1信号传导，诱导大鼠主动脉平滑肌细胞中CXCL16的表达。  |  Chandrasekar, B., et al. 2005. J Biol Chem. 280: 26263-77. PMID: 15890643
  9. 胸腺肽α1激活IKK需要TRAF6信号通路。  |  Zhang, P., et al. 2005. EMBO Rep. 6: 531-7. PMID: 15905851
  10. E3连接酶TRAF6调节Akt泛素化和激活。  |  Yang, WL., et al. 2009. Science. 325: 1134-8. PMID: 19713527
  11. PRDX1 对 TRAF6 泛素连接酶活性的抑制导致对 NFKB 激活和自噬激活的抑制。  |  Min, Y., et al. 2018. Autophagy. 14: 1347-1358. PMID: 29929436
  12. TRAF6 E3连接酶对ATG9A的K48/K63连接多泛素化调节氧化应激诱导的自噬。  |  Wang, YT., et al. 2022. Cell Rep. 38: 110354. PMID: 35196483

订购信息

产品名称产品编号规格价格数量收藏夹

TRAF6 抗体 (D-10)

sc-8409
200 µg/ml
$316.00

TRAF6 (D-10): m-IgG Fc BP-HRP 套装

sc-528225
200 µg Ab; 10 µg BP
$354.00

TRAF6 (D-10): m-IgGκ BP-HRP 套装

sc-520551
200 µg Ab, 40 µg BP
$354.00

TRAF6 (D-10): m-IgG1 BP-HRP 套装

sc-542826
200 µg Ab; 20 µg BP
$354.00

TRAF6 抗体 (D-10) AC

sc-8409 AC
500 µg/ml, 25% agarose
$416.00

TRAF6 抗体 (D-10) HRP

sc-8409 HRP
200 µg/ml
$316.00

TRAF6 抗体 (D-10) FITC

sc-8409 FITC
200 µg/ml
$330.00

TRAF6 抗体 (D-10) PE

sc-8409 PE
200 µg/ml
$343.00

TRAF6 抗体 (D-10) Alexa Fluor® 488

sc-8409 AF488
200 µg/ml
$357.00

TRAF6 抗体 (D-10) Alexa Fluor® 546

sc-8409 AF546
200 µg/ml
$357.00

TRAF6 抗体 (D-10) Alexa Fluor® 594

sc-8409 AF594
200 µg/ml
$357.00

TRAF6 抗体 (D-10) Alexa Fluor® 647

sc-8409 AF647
200 µg/ml
$357.00

TRAF6 抗体 (D-10) Alexa Fluor® 680

sc-8409 AF680
200 µg/ml
$357.00

TRAF6 抗体 (D-10) Alexa Fluor® 790

sc-8409 AF790
200 µg/ml
$357.00

I want to use this antibody for an IP. I need to lyse the cells under denaturing conditions. Is the epitope functional when TRAF6 is denatured? Can you tell me what the epitope is?

Asked by: TRAF6_IP
Thank you for your question. Yes, the epitope is functional under denaturing conditions. The epitope range is 1-274 .
Answered by: BlakeJ
Date published: 2024-08-02

Is this (TRAF6, D10) antibody good for flowcytometry? Anybody worked with FITC or AF488 conjugated one for flow in splenocytes?

Asked by: Flow
Thank you for contacting Santa Cruz Biotechnology . We do not have any publications for Flow Cytometry. We have a one year warranty from date of purchase. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-03-29

Dear all, I would like to test your traf 6 (D10) antibody (sc8409) by Western blot. Would it be possible to send me a small aliquot? Thanks is advance, kind regards Chantal

Asked by: Anonymous
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-09-30

Hello. I am a PhD student and analysing expression in inflammatory pathways. I was wondering if I could get a free sample of 10 µg sample of TRAF6 (D-10): sc-8409. Thank you in advance.

Asked by: Denise Carrasco
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-8409, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Hi, I am part of an innate immunology research laboratory and am currently in the process of obtaining an anti-TRAF6 antibody for upcoming WB, IP and IF assays. Is it possible to receive the 10ug free sample advertised above for TRAF6 (D-10): sc-8409?

Asked by: Keaton
Thank you for your question. Please contact your local distributor in Australia, they will be happy to send you any samples you wish to test. You can find their contact information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-08-14

What is a suitable positive control cell line for testing the TRAF6 Antibody (D-10): sc-8409 in Western blot?

Asked by: cjMara
Thank you for your question. We recommend using either HeLa (sc-2200) or WEHI-3 (sc-3815) whole cell lysates for Western blot testing with this TRAF6 antibody.
Answered by: Technical Support
Date published: 2016-12-21

What is the exact epitope recognized by TRAF6 (D-10): sc-8409?

Asked by: cjMara
Thank you for your question. The (D-10) clone is raised against amino acids 1-274 mapping at the N-terminus of TRAF6 of human origin. The exact epitope recognized by this mouse monoclonal antibody has not been determined.
Answered by: Technical Support
Date published: 2016-12-21

Hello, I am trying to IP (pull down) endogenous TRAF6. Which catalog# antibody would be the best option for me?

Asked by: Asadi
  • y_2025, m_8, d_27, h_5CST
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Rated 4 out of 5 by from Traf6 antibodyTraf6 (D-10) antibody was worked with 1:50-1:100 dilution on mice lung tissue by IHC staining.
Date published: 2022-12-01
Rated 5 out of 5 by from Immunofluorescence brain sectionsTried it on PFA fixed brain sections (40um thick, cut with vibratome). Worked well at 100x dilution and with 40x objective.
Date published: 2022-08-30
Rated 5 out of 5 by from Great antibodyI bought this product a week ago, and the experiment is going well. I will buy it again
Date published: 2022-08-02
Rated 4 out of 5 by from 还可以本课题组购买该抗体，并用于蛋白免疫印迹实验。抗体特异性尚可，根据组织中表达量不用，应根据情况适当调整稀释比例。
Date published: 2022-07-04
Rated 5 out of 5 by from Good Western Blot dataThis Antibody works well for western blot in the ovine liver.
Date published: 2021-11-28
Rated 5 out of 5 by from It is goodI got a clear band using this antibody at 1:1000 dilution in ovine thymus.
Date published: 2021-04-19
Rated 1 out of 5 by from No signal in WBI used this antibody on murine cell line extract and no signal was detected
Date published: 2020-11-18
Rated 4 out of 5 by from Good AntibodyI bought this antibody two months ago. Although it took time to optimize the condition, it worked out well.
Date published: 2020-07-11
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TRAF6抗体 (D-10) is rated 4.5 out of 5 by 30.
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