Date published: 2025-9-7

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TPX2 抗体 (E-2): sc-271570

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说明书
  • TPX2 抗体 E-2 是小鼠单克隆 IgG1 κ,TPX2抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列448-747位于human物种的TPX2的C-terminus
  • TPX2 抗体 (E-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 TPX2
  • 抗TPX2抗体(E-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TPX2 (E-2): sc-271570免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 TPX2 抗体 (E-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TPX2 抗体 (E-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

TPX2抗体(E-2)是一种IgG1 κ型小鼠单克隆TPX2抗体(也被指定为TPX2抗体),可通过WB, IP, IF, IHC(P)和ELISA检测人源的TPX2蛋白。TPX2抗体(E-2)既可以非共轭抗TPX2抗体的形式提供,也可以多种共轭形式的抗TPX2抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。TPX2(Xklp2的靶蛋白)是一种微管相关蛋白,参与将运动蛋白Xklp2靶向微管。在M期,Ran-GTP激活TPX2进行染色质诱导的微管组装。Aurora-A激酶在纺锤体装置上与TPX2结合,并可能在纺锤体组装过程中通过磷酸化调节TPX2。TPX2似乎在纺锤体形成中发挥结构作用。TPX2通过刺激Eg2的磷酸化和激酶活性,以微管依赖的方式激活Eg2。TPX2通过与核输入因子importin α结合而失活。最后,用RNA干扰抑制TPX2会导致有丝分裂期间微管组织的缺陷。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TPX2 抗体 (E-2) 参考文献:

  1. Ran通过逆转输入蛋白α对TPX2活性的抑制作用来诱导纺锤体组装。  |  Gruss, OJ., et al. 2001. Cell. 104: 83-93. PMID: 11163242
  2. 人类 TPX2 是将 Aurora-A 激酶靶向纺锤体的必要条件。  |  Kufer, TA., et al. 2002. J Cell Biol. 158: 617-23. PMID: 12177045
  3. HeLa细胞纺锤体的形成需要由TPX2介导的染色体诱导的微管组装。  |  Gruss, OJ., et al. 2002. Nat Cell Biol. 4: 871-9. PMID: 12389033
  4. 脊椎动物细胞分裂过程中正常的纺锤体形态和中心体完整性需要 hTPX2。  |  Garrett, S., et al. 2002. Curr Biol. 12: 2055-9. PMID: 12477396
  5. 有丝分裂激酶 Aurora A 在纺锤体组装过程中介导的 Ran 信号通路。  |  Tsai, MY., et al. 2003. Nat Cell Biol. 5: 242-8. PMID: 12577065
  6. 驱动蛋白样蛋白 Xklp2 在纺锤极的定域需要亮氨酸拉链, 微管缔合蛋白和动力蛋白。  |  Wittmann, T., et al. 1998. J Cell Biol. 143: 673-85. PMID: 9813089

订购信息

产品名称产品编号规格价格数量收藏夹

TPX2 抗体 (E-2)

sc-271570
200 µg/ml
RMB2377.00

TPX2 (E-2): m-IgG Fc BP-HRP 套装

sc-527259
200 µg Ab; 10 µg BP
RMB2662.00

TPX2 (E-2): m-IgG1 BP-HRP 套装

sc-532632
200 µg Ab; 20 µg BP
RMB2662.00

TPX2 抗体 (E-2) AC

sc-271570 AC
500 µg/ml, 25% agarose
RMB3129.00

TPX2 抗体 (E-2) HRP

sc-271570 HRP
200 µg/ml
RMB2377.00

TPX2 抗体 (E-2) FITC

sc-271570 FITC
200 µg/ml
RMB2482.00

TPX2 抗体 (E-2) PE

sc-271570 PE
200 µg/ml
RMB2580.00

TPX2 抗体 (E-2) Alexa Fluor® 488

sc-271570 AF488
200 µg/ml
RMB2685.00

TPX2 抗体 (E-2) Alexa Fluor® 546

sc-271570 AF546
200 µg/ml
RMB2685.00

TPX2 抗体 (E-2) Alexa Fluor® 594

sc-271570 AF594
200 µg/ml
RMB2685.00

TPX2 抗体 (E-2) Alexa Fluor® 647

sc-271570 AF647
200 µg/ml
RMB2685.00

TPX2 抗体 (E-2) Alexa Fluor® 680

sc-271570 AF680
200 µg/ml
RMB2685.00

TPX2 抗体 (E-2) Alexa Fluor® 790

sc-271570 AF790
200 µg/ml
RMB2685.00

Hi! This antibody can be used for mouse protein detection?

Asked by: sofiam
Thank you for your question. sc-271570 TPX2 (E-2) antibody has not been validated for mouse detection. TPX2 Antibody (E-2) is recommended for detection of TPX2 of human origin by WB, IP, IF, IHC(P) and ELISA. Here are TPX2 antibodies for mouse detection. https://www.scbt.com/p/tpx2-antibody-b-5 https://www.scbt.com/p/tpx2-antibody-b-10
Answered by: BlakeJ
Date published: 2022-11-14

Can TPX2 (E-2): sc-271570 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, TPX2 (E-2): sc-271570 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Good for IFThis TPX2 Antibody (E-2), sc-271570, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from Works in WBWe received a sample and used it in human colon carcinoma cells. We detected a band at 120 kDa in western blot. (a bit higher than expected)
Date published: 2017-10-25
Rated 4 out of 5 by from GOODThe antibody is very good for WB to detect transacted TPX2-tagged protein. works great on immunoblotting at 1:750 in TBST
Date published: 2017-09-25
Rated 4 out of 5 by from western 条带较清晰,但试用装的量没有10ulwesternblot条带较清晰,没有杂带。但是只有不到5ul的量,所以只敷了两三次,重复性还看不出
Date published: 2017-09-01
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in formalin-fixed HeLa cells. -SCBT QC
Date published: 2015-05-13
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TPX2 抗体 (E-2) is rated 4.2 out of 5 by 5.
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