Date published: 2025-9-7
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Topo IIβ Antibody (A-12) - Western Blotting - Image 147413
Topo IIβ (A-12): sc-365071. U-937 (A), MEG-01 (B), CCRF-CEM (C) 和 K-562 (D) 核提取物中 Topo IIβ 表达的 Western 印迹分析.
Topo IIβ (A-12): sc-365071. U-937 (A), MEG-01 (B), CCRF-CEM (C) 和 K-562 (D) 核提取物中 Topo IIβ 表达的 Western 印迹分析.

Topo IIβ 抗体 (A-12): sc-365071

4.2(5)
寫評論提問

说明书
  • Topo IIβ抗体(A-12)是小鼠单克隆IgG2a κ, 在8篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Topo IIβ的C-terminus附近的氨基酸1579-1611之间
  • 抗-Topo II beta 抗体 (A-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Topo IIβ
  • 抗Topo II beta抗体（A-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Topo IIβ (A-12): sc-365071的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Topo IIβ 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Topo IIβ 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Topo 抗体 包括 Topo I, Topo II, Topo II α, Topo II β和Topo III β-1

Topo IIβ抗体（A-12）是一种IgG2a κ小鼠单克隆Topo IIβ抗体（也称为DNA拓扑异构酶IIβ抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的Topo IIβ。 Topo IIβ抗体（A-12）可作为非偶联抗Topo IIβ抗体形式，以及多种偶联形式的抗Topo IIβ抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。 DNA拓扑异构酶I和II（Topo I和Topo II）是核酶，通过瞬时断裂和重新连接DNA链来调节真核细胞中DNA的拓扑结构。真核拓扑异构酶能够松弛正负超螺旋，而原核拓扑异构酶仅能松弛负超螺旋。 DNA拓扑异构酶在DNA复制、重组和转录中发挥作用，已被确定为许多抗癌药物的靶标。 Topo I是一种普遍表达的可溶性酶，通过在DNA链中引入瞬时断裂起作用，而Topo II通过形成瞬时双链断裂起作用。 Topo II由两个不同的基因编码，产生两种不同的同工型，称为Topo IIα和Topo IIβ。 Topo IIα和Topo IIβ在其N端四分之三处具有高度同源性，但是C端片段差异很大，表明这些区域可能介导不同的细胞功能，并解释了两种同工型观察到的组织表达模式的差异。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Topo IIβ 抗体 (A-12) 参考文献:

  1. DNA 拓扑异构酶 IIbeta 与神经发育  |  Yang, X., et al. 2000. Science. 287: 131-4. PMID: 10615047
  2. DNA 拓扑异构酶 IIbeta 参与神经元分化  |  Tsutsui, K., et al. 2001. J Biol Chem. 276: 5769-78. PMID: 11106659
  3. 拓扑异构酶 IIbeta 能激活在富含 AT 的基因组环境中受到抑制的神经元基因。  |  Sano, K., et al. 2008. PLoS One. 3: e4103. PMID: 19116664
  4. 作为拓扑异构酶 IIβ 毒素和细胞毒剂的新型羊栖菜酮-羊栖菜碱相关杂交种。  |  Zagotto, G., et al. 2014. Arch Pharm (Weinheim). 347: 728-37. PMID: 25042690
  5. DNA拓扑异构酶IIβ通过调控Rho-GTP酶（RhoA/Rock2通路）和Nurr1的表达刺激神经分化的人类间充质干细胞的神经元生长。  |  Zaim, M. and Isik, S. 2018. Stem Cell Res Ther. 9: 114. PMID: 29695291
  6. 拓扑异构酶 IIβ 的突变会导致 B 细胞免疫缺陷。  |  Broderick, L., et al. 2019. Nat Commun. 10: 3644. PMID: 31409799
  7. 拓扑异构酶 IIβ 在多柔比星心脏保护剂右雷佐生的作用机制中的作用  |  Hasinoff, BB., et al. 2020. Cardiovasc Toxicol. 20: 312-320. PMID: 31773441
  8. 在帕金森病细胞模型中，通过下调酪氨酸羟化酶，DNA拓扑异构酶 IIβ 通过 Rho GTPases 发挥类似神经退行性病变的作用。  |  Yeman, KB. and Isik, S. 2021. Neurol Res. 43: 464-473. PMID: 33402057
  9. 通过结构因子和染色质可及性对拓扑异构酶 IIβ 结合的全基因组预测  |  Martínez-García, PM., et al. 2021. PLoS Comput Biol. 17: e1007814. PMID: 33465072
  10. 右雷佐生类似物的结构-活性关系研究发现，ICRF-193 是抗蒽环类药物对心肌细胞毒性作用最强的双氧哌嗪，因为它与拓扑异构酶 IIβ 有很强的相互作用。  |  Jirkovská, A., et al. 2021. J Med Chem. 64: 3997-4019. PMID: 33750129

订购信息

产品名称产品编号规格价格数量收藏夹

Topo IIβ 抗体 (A-12)

sc-365071
200 µg/ml
RMB2377.00

Topo IIβ (A-12): m-IgGκ BP-HRP 套装

sc-522130
200 µg Ab, 40 µg BP
RMB2662.00

Topo IIβ 抗体 (A-12) AC

sc-365071 AC
500 µg/ml, 25% agarose
RMB3129.00

Topo IIβ 抗体 (A-12) HRP

sc-365071 HRP
200 µg/ml
RMB2377.00

Topo IIβ 抗体 (A-12) FITC

sc-365071 FITC
200 µg/ml
RMB2482.00

Topo IIβ 抗体 (A-12) PE

sc-365071 PE
200 µg/ml
RMB2580.00

Topo IIβ 抗体 (A-12) Alexa Fluor® 488

sc-365071 AF488
200 µg/ml
RMB2685.00

Topo IIβ 抗体 (A-12) Alexa Fluor® 546

sc-365071 AF546
200 µg/ml
RMB2685.00

Topo IIβ 抗体 (A-12) Alexa Fluor® 594

sc-365071 AF594
200 µg/ml
RMB2685.00

Topo IIβ 抗体 (A-12) Alexa Fluor® 647

sc-365071 AF647
200 µg/ml
RMB2685.00

Topo IIβ 抗体 (A-12) Alexa Fluor® 680

sc-365071 AF680
200 µg/ml
RMB2685.00

Topo IIβ 抗体 (A-12) Alexa Fluor® 790

sc-365071 AF790
200 µg/ml
RMB2685.00

Topo IIβ (A-12) 中和勝肽

sc-365071 P
100 µg/0.5 ml
RMB511.00

Is this antibody available as ChIP grade?

Asked by: Amit5629489
Thank you for your question. We should be able to provide this product as a TransCruz reagent for Gel Supershift and ChIP applications. Please get in touch with our technical service so we can make the necessary arrangements.
Answered by: Tech Support Europe
Date published: 2020-06-30

What application is the blocking peptide sc-365071 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from A good result for Topo IIβ WBGastric cancer cells BGC823 were uesd to confirm the expression of Topo IIβ. Transfering time was 360 minutes in 250mA. Blocking in milk for hour. Incubateing with first antibody(1:500) overnight. Incubateing with second antibody(1:2000) for one hour
Date published: 2017-11-18
Rated 2 out of 5 by from TOPOIIB A-12 in Western BlotI received this as a free sample antibody and tested it out at a 1:200 dilution using Whole Cell Extracts from four different cell lines (HeLa, HEK293, HEK293T, and Mouse Embryonic Fibroblast cells). The expected size for TOPO11B is 180 kDa and I do see something at the correct height in maybe the last 3 lanes (the HeLa could just be loaded with less extract). The exposure of the blots was weird for this antibody and another Topoisomerase antibody I also tested (Santa Cruz H-8).
Date published: 2017-10-20
Rated 5 out of 5 by from versatile and consistent!This monoclonal Topo II beta Antibody (A-12) is a versatile reagent with strong in-house data in Western blot, IF and IHC(P).
Date published: 2017-01-31
Rated 5 out of 5 by from Great immunofluorescence nuclear stainingGreat immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-02
Rated 5 out of 5 by from Nice Western Blot data of Topo II expression in UNice Western Blot data of Topo II expression in U-937, MEG-01, CCRF-CEM and K-562 nuclear extracts. -SCBT QC
Date published: 2013-02-23
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Topo IIβ 抗体 (A-12) is rated 4.2 out of 5 by 5.
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