Date published: 2025-9-9
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Tom20 Antibody (F-10) - Western Blotting - Image 390869
Tom20 抗体 (F-10) Alexa Fluor® 647: sc-17764 AF647. 对 Raji (A), NAMALWA (B), BJAB (C), CCRF-CEM (D), HeLa (E) 和 SK-N-MC (F) 全细胞裂解液中 Tom20 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Tom20 抗体 (F-10) Alexa Fluor® 647: sc-17764 AF647. 对 Raji (A), NAMALWA (B), BJAB (C), CCRF-CEM (D), HeLa (E) 和 SK-N-MC (F) 全细胞裂解液中 Tom20 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Tom20 抗体 (F-10): sc-17764

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说明书
  • Tom20抗体(F-10)是小鼠单克隆IgG2a λ Tom20抗体, 在695篇文献中引用，规格为200 µg/ml
  • 针对Tom20的氨基酸1-145 human起源
  • Tom20 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Tom20
  • 抗Tom20抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tom20 (F-10): sc-17764的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGλ BP-HRP 是 Tom20 抗体 (F-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Tom20 抗体 (F-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Tom 抗体 包括 Tom1, Tom1L-1, Tom20, Tom22, Tom34, Tom40, Tom6和Tom70

Tom20抗体（F-10）是一种小鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源Tom20蛋白。抗Tom20抗体（F-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。作为线粒体外膜转运复合物（TOM）的组成部分，Tom20在线粒体功能中起着至关重要的作用，该复合物对于将细胞核编码的前体蛋白导入线粒体至关重要。具体而言，Tom20作为导入受体，识别并结合细胞质中的这些前体蛋白，促进它们穿过线粒体外膜。这个过程对于维持线粒体功能和能量产生至关重要，因为线粒体代谢和呼吸所需的许多蛋白质是在细胞质中合成的，必须被导入线粒体。Tom20与TOM复合体其他成分（如Tom22和Tom40）之间的相互作用确保了这些蛋白质的高效运输，凸显了Tom20在细胞能量稳态中的重要性。Tom20 (F-10) 单克隆抗体是哺乳动物 Tom20 蛋白的特异性抗体，因此抗 Tom20 抗体 (F-10) 对于研究线粒体动力学和蛋白质导入机制的研究人员来说是一种宝贵的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tom20 抗体 (F-10) 参考文献:

  1. 线粒体导入受体 Tom20, Tom22 和 Tom70 的结合序列在携带前序的前蛋白和不可裂解的前蛋白中的分布。  |  Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
  2. 线粒体 TOM 复合物核心成分 Tom40 的生物生成。  |  Rapaport, D. and Neupert, W. 1999. J Cell Biol. 146: 321-31. PMID: 10427088
  3. Tom22 是线粒体前蛋白转运酶的多功能组织者。  |  van Wilpe, S., et al. 1999. Nature. 401: 485-9. PMID: 10519552
  4. TOM 核心复合体：线粒体外膜的一般蛋白质导入孔。  |  Ahting, U., et al. 1999. J Cell Biol. 147: 959-68. PMID: 10579717
  5. 线粒体外膜导入前蛋白的运输机械。  |  Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
  6. 线粒体蛋白质导入。Tom40 通过与前序形成特异性结合位点，在前体蛋白的靶向和转运中发挥着重要作用。  |  Rapaport, D., et al. 1997. J Biol Chem. 272: 18725-31. PMID: 9228044
  7. 人类线粒体受体 Tom20 将蛋白质导入线粒体的功能分析。  |  Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929
  8. 线粒体外膜的前蛋白转运酶：一般进口孔复合体的分子剖析和组装。  |  Dekker, PJ., et al. 1998. Mol Cell Biol. 18: 6515-24. PMID: 9774667

订购信息

产品名称产品编号规格价格数量收藏夹

Tom20 抗体 (F-10)

sc-17764
200 µg/ml
RMB2377.00

Tom20 (F-10): m-IgGλ BP-HRP 套装

sc-520641
200 µg Ab, 40 µg BP
RMB2662.00

Tom20 (F-10): m-IgG2a BP-HRP 套装

sc-546232
200 µg Ab; 10 µg BP
RMB2662.00

Tom20 抗体 (F-10) AC

sc-17764 AC
500 µg/ml, 25% agarose
RMB3129.00

Tom20 抗体 (F-10) HRP

sc-17764 HRP
200 µg/ml
RMB2377.00

Tom20 抗体 (F-10) FITC

sc-17764 FITC
200 µg/ml
RMB2482.00

Tom20 抗体 (F-10) PE

sc-17764 PE
200 µg/ml
RMB2580.00

Tom20 抗体 (F-10) Alexa Fluor® 488

sc-17764 AF488
200 µg/ml
RMB2685.00

Tom20 抗体 (F-10) Alexa Fluor® 546

sc-17764 AF546
200 µg/ml
RMB2685.00

Tom20 抗体 (F-10) Alexa Fluor® 594

sc-17764 AF594
200 µg/ml
RMB2685.00

Tom20 抗体 (F-10) Alexa Fluor® 647

sc-17764 AF647
200 µg/ml
RMB2685.00

Tom20 抗体 (F-10) Alexa Fluor® 680

sc-17764 AF680
200 µg/ml
RMB2685.00

Tom20 抗体 (F-10) Alexa Fluor® 790

sc-17764 AF790
200 µg/ml
RMB2685.00

Hi. Can i use this antibody for immunostaining in IPSC- cardiomyocytes? Do you have article that has used this antibody in IPSC cells and do you recommend in WB?

Asked by: Thika
Thank you for your question. We have not tested on IPSC cells but the antibody is recommended for mouse, rat, and human samples. The antibody is recommended for western blot, IP and ELISA.
Answered by: BlakeJ
Date published: 2025-09-01

Hello! Can I use this antibody for Flow Cytometry? If so, with what dilution?

Asked by: TOM20 for Flow Cytometry
Thank you for your question. Fluorescent conjugates of Tom20 Antibody (F-10): sc-17764 can be used for flow cytometry. We recommend using 1 µg of fluorochrome-conjugated antibody per 1 million cells in a volume of 100 µl per tube.
Answered by: Tech Support Europe
Date published: 2023-09-28

What is the epitope sequence for this antibody? Is it the whole Tom20 protein sequence?

Asked by: Jaslin
Thank you for your question the sequence is proprietary . The antibody was raised against amino acids 1-145 of Tom20 human origin.
Answered by: BlakeJ
Date published: 2023-06-16

What fixative is recommended for this antibody? Can I use 4% PFA? Trichloroacetic Acid (TCA)?

Asked by: Jaslin
Thank you for your question. We recommend fixation in methanol, acetone or formalin. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2023-06-16

Can I use Tom20 抗体 (F-10): sc-17764 in pig ?

Asked by: TANGYY
Thank you for your question.Tom20 Antibody (F-10) is recommended for detection of Tom20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.It is not recommended for pigs.Thank you.
Answered by: Jiajun Z
Date published: 2023-03-14

Do you have an antibody like sc-11415 against TOMM20 & produced in rabbit?

Asked by: erorivera
Thank you for your question. We currently do not have such an antibody produced in rabbit. Please refer to our monoclonal alternative Anti-Tom20 Antibody (F-10): sc-17764 instead. This antibody is available in different conjugation variants, which opens up options for co-staining and significantly reduces the risk of unspecific staining.
Answered by: Tech Support Europe
Date published: 2022-11-03

hi, Sir or Madam, your website showed Tom20 antibody in your company also reacted with the drosophila in WB, can you send me the article that has ever used this antibody in flies? thanks a lot.

Asked by: fifilu
Thank you for your question. Anti-Tom20 Antibody (F-10): sc-17764 was not tested for Drosophila and we do not have any data on it's functionality in these samples. It is recommended for detection of Tom20 of mouse, rat and human origin
Answered by: Tech Support Europe
Date published: 2022-03-02

this antibody should replace the old polyclonal "sc-11415" antibody. However, this one is mouse, and i am looking for an antibody coming from rabbit. Is it possible to still order the old antibody?

Asked by: Marisaa
Thank you for your question. Unfortunately we do not offer sc-11415, and are unable to provide it for you. We are a monoclonal specific company and do not provide rabbit antibodies at this time.
Answered by: Tech Service
Date published: 2019-10-14
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Rated 4 out of 5 by from Works well for IF on brain organoid sectionsGood IF signal in sections of OCT-included human brain organoids. The antibody was used at a dilution of 1:100. Antigen retrieval with EDTA 1mM was performed on the sections before the staining.
Date published: 2025-03-07
Rated 5 out of 5 by from Great for IFExcellent outer mitochondrial marker in IF experiments. We use 1:100 dilution at RT (90 min incubation).
Date published: 2025-03-04
Rated 1 out of 5 by from Did not work in IFUnfortunately it did not work in immunofluorescence of mouse primary neurons.
Date published: 2025-01-27
Rated 5 out of 5 by from good stainingStrong stainig of human tissue, high expressed,1:500 dilution.
Date published: 2024-06-03
Rated 5 out of 5 by from Tom20 worked very well in IHC of human tissuesTom20 worked very well in histology of human tissues by IF
Date published: 2024-05-30
Rated 5 out of 5 by from Excelente para Western Blot e InmunofluorescenciaProbamos este anticuerpo para WB e IF y los resultados han sido muy buenos. Muy buenas marcas y especificidad a bajas concentraciones.
Date published: 2023-04-19
Rated 5 out of 5 by from Great specificity in immunofluorescent stainingI bought this antibody earlier and used it to stain mitochondria in adipocytes. The IF staining was great in terms of specificity and signal/noise ratio.
Date published: 2023-03-14
Rated 5 out of 5 by from IF for mouse tissueWorks great for IF of mouse tissue especially when you do double stain with rabbit antibody
Date published: 2022-09-24
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Tom20 抗体 (F-10) is rated 4.4 out of 5 by 76.
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