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Tollip Antibody (Kimmy-2) - Western Blotting - Image 56377
Tollip 抗体 (Kimmy-2): sc-59720. 对 SK-N-MC 全细胞裂解液中 Tollip 的表达进行 Western 印迹分析.
Tollip Antibody (Kimmy-2) - Western Blotting - Image 56377
Tollip 抗体 (Kimmy-2): sc-59720. 对 SK-N-MC 全细胞裂解液中 Tollip 的表达进行 Western 印迹分析.
Tollip Antibody (Kimmy-2) - Western Blotting - Image 45976
Tollip 抗体 (Kimmy-2): sc-59720. 未转染 293T: sc-117752 (A), 小鼠 Tollip 转染 293T: sc-127686 (B) 和 EOC 20 (C) 全细胞裂解液中 Tollip 表达的 Western 印迹分析.
Tollip Antibody (Kimmy-2) - Western Blotting - Image 20189
Tollip 抗体 (Kimmy-2): sc-59720. 非转染: sc-110760 (A) 和人 Tollip 转染: sc-112320 (B) 293 全细胞裂解液中 Tollip 表达的 Western 印迹分析.
Tollip 抗体 (Kimmy-2): sc-59720. 对 SK-N-MC 全细胞裂解液中 Tollip 的表达进行 Western 印迹分析.

Tollip 抗体 (Kimmy-2): sc-59720

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说明书
  • Tollip 抗体 Kimmy-2 是小鼠单克隆 IgG1，Tollip抗体，规格为50 µg/0.5 ml
  • 与对应mouse来源的Tollip的氨基酸1-247的全长重组蛋白杂交
  • 推荐用于 mouse, rat 和 human 来源的Tollip WB, IP, IF 和 IHC(P)检测
  • 关于如何获取Tollip (Kimmy-2): sc-59720的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Tollip 抗体 (Kimmy-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    Tollip抗体（Kimmy-2）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的Tollip蛋白。抗Tollip抗体（Kimmy-2）以非结合形式提供。Tollip，即Toll相互作用蛋白，通过作为Toll样受体（TLR）途径的负调节因子，在调节先天免疫信号方面发挥着关键作用。具体而言，Tollip与丝氨酸/苏氨酸激酶IRAK相互作用，在受体激活时将它与IL-1受体复合物联系起来，这对控制炎症反应至关重要。Tollip在HEK293细胞中的过度表达会抑制NFκB对TLR2和TLR4信号的激活，从而限制炎症和感染期间促炎性介质的生产。此外，Tollip与Tom1形成复合物，这对调节泛素化蛋白的内吞体运输至关重要，凸显了其在细胞过程中的重要性。Tollip在小鼠组织中普遍表达，强调了其在各种生物学背景下免疫调节中的基础作用。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Tollip 抗体 (Kimmy-2) 参考文献:

    1. Tollip是IL-1RI通路的一个新成分，它将IRAK与IL-1受体连接起来。  |  Burns, K., et al. 2000. Nat Cell Biol. 2: 346-51. PMID: 10854325
    2. Toll样受体2和6在可溶性结核因子和包柔氏菌外表面蛋白A脂蛋白激活细胞方面的合作：Toll-interacting蛋白和IL-1受体信号分子在Toll样受体2信号传导中的作用。  |  Bulut, Y., et al. 2001. J Immunol. 167: 987-94. PMID: 11441107
    3. Tollip 对收费样受体介导的信号转导的负向调节。  |  Zhang, G. and Ghosh, S. 2002. J Biol Chem. 277: 7059-65. PMID: 11751856
    4. Tollip 和 Tom1 形成一个复合物，并将泛素连接蛋白招募到早期内体上。  |  Katoh, Y., et al. 2004. J Biol Chem. 279: 24435-43. PMID: 15047686
    5. 脂多糖挑战下 Tollip 蛋白的特征。  |  Li, T., et al. 2004. Mol Immunol. 41: 85-92. PMID: 15140579
    6. CD26介导Tollip和IRAK-1从caveolin-1上分离，并诱导抗原呈递细胞上调CD86。  |  Ohnuma, K., et al. 2005. Mol Cell Biol. 25: 7743-57. PMID: 16107720
    7. Tom1-Tollip复合体将凝集素募集到内体上。  |  Katoh, Y., et al. 2006. Biochem Biophys Res Commun. 341: 143-9. PMID: 16412388
    8. Tollip 可调节对白细胞介素-1 和脂多糖的促炎反应。  |  Didierlaurent, A., et al. 2006. Mol Cell Biol. 26: 735-42. PMID: 16428431
    9. toll-interacting 蛋白基因多态性与特应性皮炎的关系。  |  Schimming, TT., et al. 2007. BMC Dermatol. 7: 3. PMID: 17362526
    10. Tollip 可协调巨噬细胞极化，缓解肠黏膜炎症。  |  Liu, X., et al. 2022. J Crohns Colitis. 16: 1151-1167. PMID: 35134154
    11. 抑制 ACE2 SUMOylation 可通过 TOLLIP 介导的选择性自噬防止 SARS-CoV-2 感染。  |  Jin, S., et al. 2022. Nat Commun. 13: 5204. PMID: 36057605
    12. α-疱疹病毒的保护膜蛋白UL21通过TOLLIP介导的选择性自噬引发CGAS降解，从而抑制先天性免疫。  |  Ma, Z., et al. 2023. Autophagy. 19: 1512-1532. PMID: 36343628

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Tollip 抗体 (Kimmy-2)

    sc-59720
    50 µg/0.5 ml
    		RMB2377.00

    Hi, I want to know whether this antibody can detect other isoforms or not? Thanks.

    Asked by: Peggy H
    Thank you for your question. We have not specifically tested this monoclonal antibody, Tollip (Kimmy-2): sc-59720, to determine potential cross-reactivity with different isoforms of Tollip. Based on the Western blot data published on our website, the antibody does detect the full-length protein (isoform 1) in the cell lines and transfected lysates tested.
    Answered by: Technical Support
    Date published: 2017-10-06

    Hi, where is the epitope of this antibody specific location [sc-59720: Tollip (Kimmy-2)]? N-terminal, C-terminal or other place?

    Asked by: Peggy H
    Thank you for your question. This mouse monoclonal antibody, Tollip (Kimmy-2): sc-59720, is raised against a full-length recombinant protein corresponding to amino acids 1-247 of Tollip of mouse origin. The exact epitope recognized by this antibody has not been determined.
    Answered by: Technical Support
    Date published: 2017-10-06

    Can sc-59720: Tollip (Kimmy-2) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Dr Ninau Qelp
    Thank you for your question. Yes, sc-59720: Tollip (Kimmy-2) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 5 out of 5 by from Specific for Tollip in Western BlotAntibody detects over-expressed human and mouse Tollip in transfected lysates sc-112320 and sc-127686, respectively. -SCBT QC
    Date published: 2023-09-14
    Rated 2 out of 5 by from work for WBI detected a weak band around 36kDa and other several non specific bands.
    Date published: 2021-04-21
    Rated 2 out of 5 by from good for PLA proximity ligationThis antibody is ok for PLA proximity ligation assay. However, it is not very good to use for WB. I diluted the antibody 1:200, and used very high concentration of lysate on the gel. However, the antibody can't detect the protein very well. There were multiple bands and very faint. It took a long time to detect a faint band.
    Date published: 2019-01-11
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    Tollip 抗体 (Kimmy-2) is rated 3.0 out of 5 by 4.
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