Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
TNIK Antibody (C-1) - Immunohistochemistry - Image 370322
TNIK 抗体 (C-1): sc-377215. 福尔马林固定, 石蜡包埋的人小肠组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
TNIK 抗体 (C-1): sc-377215. 福尔马林固定, 石蜡包埋的人小肠组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.

TNIK 抗体 (C-1): sc-377215

5.0(4)
寫評論提問

说明书
  • TNIK 抗体 C-1 是小鼠单克隆 IgG2b κ，TNIK抗体, 在4篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列844-922位于human物种的TNIK的内部区域内
  • TNIK 抗体 (C-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TNIK
  • 抗TNIK抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TNIK (C-1): sc-377215的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 TNIK 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TNIK 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

TNIK抗体（C-1）是一种IgG2b κ小鼠单克隆TNIK抗体（也称为TRAF2和NCK相互作用蛋白激酶抗体，或MRT54抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的TNIK蛋白。TNIK抗体（C-1）既有非共轭型抗TNIK抗体形式，也有多种共轭型抗TNIK抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。在真核生物中，丝氨酸和苏氨酸残基上蛋白质的磷酸化和去磷酸化是调节细胞分裂、稳态和凋亡等多种细胞功能的重要手段。一组与此过程密切相关的蛋白质是丝氨酸/苏氨酸（Ser/Thr）蛋白激酶。TNIK（TRAF2和NCK相互作用蛋白激酶）是一种含有1360个氨基酸的蛋白质，含有一个蛋白激酶结构域，属于Ser/Thr蛋白激酶的一个亚家族。TNIK在脑、心脏和骨骼肌中表达量最高，且无处不在，它作为一种应激激活的Ser/Thr激酶，催化ATP依赖性的靶蛋白磷酸化，被认为在环境应激反应中发挥作用。此外，TNIK通过其催化活性，可能参与整个细胞的细胞骨架调节事件。TNIK有8种亚型，由选择性剪接事件产生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TNIK 抗体 (C-1) 参考文献:

  1. Wnt信号通路中的治疗靶点：在结直肠癌中靶向TNIK的可行性。  |  Masuda, M., et al. 2015. Pharmacol Ther. 156: 1-9. PMID: 26542362
  2. TNIK抑制消除了结肠直肠癌的干细胞特性。  |  Masuda, M., et al. 2016. Nat Commun. 7: 12586. PMID: 27562646
  3. TNIK抑制剂在癌症治疗中的应用。  |  Yamada, T. and Masuda, M. 2017. Cancer Sci. 108: 818-823. PMID: 28208209
  4. TNIK 上调对耐 JQ1 人结肠直肠癌 HCT116 细胞的影响。  |  Takahashi, C., et al. 2020. Biochem Biophys Res Commun. 530: 230-234. PMID: 32828291
  5. TNIK 是肺鳞癌的治疗靶点，通过 Merlin 调节 FAK 的活化  |  Torres-Ayuso, P., et al. 2021. Cancer Discov. 11: 1411-1423. PMID: 33495197
  6. TNIK影响抗精神病药对Wnt和β-catenin信号通路的影响。  |  Yuan, R., et al. 2021. Psychopharmacology (Berl). 238: 3283-3292. PMID: 34350475
  7. LKB1缺失通过调节TNIK表达和重塑肌动蛋白细胞骨架促进结直肠癌细胞转移。  |  Hu, G., et al. 2023. Mol Carcinog. 62: 1659-1672. PMID: 37449799
  8. TNIK调节干扰素信号和内皮细胞对病毒感染的反应。  |  Chau, KM., et al. 2023. Front Cardiovasc Med. 10: 1213428. PMID: 38264262
  9. TNIK抑制使TNIK过表达的肺鳞状细胞癌对放射治疗敏感。  |  Nguyen, T., et al. 2024. Mol Cancer Ther.. PMID: 38670554
  10. TNIK抑制使TNIK过表达的肺鳞状细胞癌对放射治疗敏感。  |  Nguyen, T., et al. 2024. Mol Cancer Ther. OF1-OF11. PMID: 38853421

订购信息

产品名称产品编号规格价格数量收藏夹

TNIK 抗体 (C-1)

sc-377215
200 µg/ml
$316.00

TNIK (C-1): m-IgGκ BP-HRP 套装

sc-523235
200 µg Ab, 40 µg BP
$354.00

TNIK (C-1): m-IgG2b BP-HRP 套装

sc-549098
200 µg Ab; 10 µg BP
$354.00

TNIK 抗体 (C-1) AC

sc-377215 AC
500 µg/ml, 25% agarose
$416.00

TNIK 抗体 (C-1) HRP

sc-377215 HRP
200 µg/ml
$316.00

TNIK 抗体 (C-1) FITC

sc-377215 FITC
200 µg/ml
$330.00

TNIK 抗体 (C-1) PE

sc-377215 PE
200 µg/ml
$343.00

TNIK 抗体 (C-1) Alexa Fluor® 488

sc-377215 AF488
200 µg/ml
$357.00

TNIK 抗体 (C-1) Alexa Fluor® 546

sc-377215 AF546
200 µg/ml
$357.00

TNIK 抗体 (C-1) Alexa Fluor® 594

sc-377215 AF594
200 µg/ml
$357.00

TNIK 抗体 (C-1) Alexa Fluor® 647

sc-377215 AF647
200 µg/ml
$357.00

TNIK 抗体 (C-1) Alexa Fluor® 680

sc-377215 AF680
200 µg/ml
$357.00

TNIK 抗体 (C-1) Alexa Fluor® 790

sc-377215 AF790
200 µg/ml
$357.00

I'm planning to use this antibody for an immunofluorescence (IF) experiment. However, there are no example IF results available for this product. I want to verify my experimental results using accurate IF data for this product. How can I obtain an exampl

Asked by: YOUME
Thank you for your question. It would be helpful if you could email us directly at [email protected]. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2024-11-12

I would like to use Anti-TNIK Antibody (C-1) sc-377215 for western blot. Any suggestion on the use of BSA or Milk for blocking and antibody incubation?

Asked by: Sieggi
Thank you for your question. We recommend blocking with 3% BSA or 5% milk and incubating the antibody for 1 hr at room temp to overnight at 4ºC. Here is a link to our Western blot protocol https://www.scbt.com/resources/protocols/western-immuno-blotting If you have additional questions, please contact Technical Support by calling 1800-457-3801 option 2, by emailing [email protected] or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Blake J
Date published: 2021-07-19

we would like to perform an immunohistochemical staining for TNIK. I found this very interesting monoclonal antibody, offered by you. Now, I was wondering if I should use the pure TNIK Antibody(C-1)(sc-377215) or the HRP-conjugated one?

Asked by: Stefan_F
Thank you for your question. You can use either one. If you use the HRP conjugated form, you do not need to use a secondary antibody, which may be of advantage, but you also can not decide to use the antibody for another application such as immunolfluorescence for example since it's already conjugated. Most customers get the unconjugated form and elect to use a secondary, but either way will work beautifully. Please feel free to reach out to Technical Service if you wish to discuss further, we're happy to help you decide!
Answered by: Tech Service
Date published: 2020-01-28

Has Santa Cruz done any testing for whether this antibody is specific to TNIK as opposed to the highly related kinases MINK1 and MAP4K4?

Asked by: mnm1
Thank you for your question. Yes, this mouse monoclonal antibody, TNIK (C-1): sc-377215, has been validated for specificity using our CRISPR/Cas9 KO Plasmids against the same target gene, TNIK of human origin. This data is shown on our website.
Answered by: Technical Support
Date published: 2017-10-24

I am using TNIK (C-1): sc-377215 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_8, d_29, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_377215, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 110ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Antibodies nicely working for WB and IPImage shows analysis of TNIK level in HEK293 cells upon silencing of TNIK gene using siRNAs. TNIK Antibodies (C-1): sc-377215 detect a band of approximately 180 kDa (corresponding to molecular weight of TNIK) and are specific.
Date published: 2019-02-22
Rated 5 out of 5 by from Nice and specificNice, strong and specific results produced by IHCP and WB. Also, TNIK (C-1) antibody produced expected results when used as a control antibody for CRISPR KO experiment.
Date published: 2017-01-27
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Great Western Blot data of TNIK expression in SKGreat Western Blot data of TNIK expression in SK-MEL-28 whole cell lysate. -SCBT QC
Date published: 2013-03-27
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_377215, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 20ms
  • REVIEWS, PRODUCT
TNIK 抗体 (C-1) is rated 5.0 out of 5 by 4.
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_377215, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。