Date published: 2026-10-24

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TNF alpha 抗体 (C-4): sc-133192

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说明书
  • TNF alpha抗体(C-4)是小鼠单克隆IgG2a κ, 在54篇文献中引用,规格为200 µg/ml
  • 免疫human物种的TNFα的氨基酸77-233
  • TNF alpha 抗体 (C-4) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 TNFα
  • 抗TNF alpha抗体(C-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是TNF alpha Antibody (C-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与TNF alpha Antibody (C-4) 打包提供(请参阅下面的订购信息)。
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TNFα抗体(C-4)是一种IgG2a κ小鼠单克隆TNFα抗体(也称为TNFα抗体或TNF alpha抗体),可通过WB、IP、IF和ELISA检测人源TNFα。TNFα抗体(C-4)可作为非偶联抗TNFα抗体形式,以及多种偶联形式的抗TNFα抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肿瘤坏死因子β(TNFβ),也称为淋巴毒素,是一种多效性细胞因子。TNFα,也称为恶病质素,是一种较小的细胞因子,可与相同的受体结合,产生与TNFβ相似的一系列广泛效应。TNFβ和TNFα具有30%的氨基酸同源性,并具有相似的生物学活性。TNFβ由活化的淋巴细胞产生,包括CD4+ T辅助细胞1型淋巴细胞、CD8+淋巴细胞和某些B淋巴母细胞系。TNFα由几种不同的细胞类型产生,包括淋巴细胞、中性粒细胞和巨噬细胞。TNFα和TNFβ可以调节许多免疫和炎症功能,同时具有抑制肿瘤生长的能力。靶肿瘤细胞必须表达TNF受体1和2才能被杀死,而p55受体介导细胞毒性反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TNF alpha 抗体 (C-4) 参考文献:

  1. 肿瘤坏死因子-α(TNF-α)在季节性过敏性结膜炎和春季角膜炎中的作用。  |  Leonardi, A., et al. 2003. Eur J Ophthalmol. 13: 606-10. PMID: 14552593
  2. 在不育症调查中,精浆中肿瘤坏死因子α(TNF-α)和白细胞介素1-β(IL-1β)的测定作用。  |  Eggert-Kruse, W., et al. 2007. Fertil Steril. 87: 810-23. PMID: 17430733
  3. 肿瘤坏死因子-α(TNF-α)可增强人体滑膜细胞中 TRP 阳离子通道的功能性热反应和化学反应。  |  Kochukov, MY., et al. 2009. Mol Pain. 5: 49. PMID: 19695100
  4. 类风湿性关节炎患者肿瘤坏死因子-α(TNF-α)-308和-238 G/A多态性与共享表位状态及对TNF-α阻断剂反应性之间的关系:荟萃分析更新。  |  Lee, YH., et al. 2010. J Rheumatol. 37: 740-6. PMID: 20194454
  5. 肿瘤坏死因子α(TNF-α)抑制剂的特征:与 TNF 受体免疫交叉反应的证据。  |  Seckinger, P., et al. 1990. Proc Natl Acad Sci U S A. 87: 5188-92. PMID: 2164226
  6. 在癌症治疗中揭示跨膜TNFα动态。  |  Bhattacharyya, S. and Ghosh, SS. 2021. Cytokine. 137: 155303. PMID: 33002738
  7. 肿瘤坏死因子α基因(TNF-α)多态性-308G/A与复发性多发性硬化症患者TNF-α血清水平的关系。  |  Grigorova, AA., et al. 2021. Neurol Res. 43: 291-298. PMID: 33252003
  8. 西他沙星可降低肿瘤坏死因子α(TNFα)转化酶(TACE)的磷酸化水平和活性,从而抑制脂多糖刺激的THP-1细胞释放TNFα。  |  Sakamaki, I., et al. 2021. Sci Rep. 11: 24154. PMID: 34921186
  9. 一般治疗银屑病患者的血清中肿瘤坏死因子α(TNFα)和精氨酰精氨酸脱亚胺酶4(PAD-4)水平的变化。  |  Czerwińska, J., et al. 2022. Int J Environ Res Public Health. 19: PMID: 35886575
  10. 肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)在B淋巴细胞功能中的作用。  |  Rieckmann, P., et al. 1997. Methods. 11: 128-32. PMID: 8990098

订购信息

产品名称产品编号规格价格数量收藏夹

TNF alpha 抗体 (C-4)

sc-133192
200 µg/ml
CNY2422.00

TNF alpha (C-4): m-IgG Fc BP-HRP 套装

sc-528765
200 µg Ab; 10 µg BP
CNY2715.00

TNF alpha (C-4): m-IgGκ BP-HRP 套装

sc-521270
200 µg Ab, 40 µg BP
CNY2715.00

TNF alpha (C-4): m-IgG2a BP-HRP 套装

sc-547163
200 µg Ab; 10 µg BP
CNY2715.00

TNF alpha 抗体 (C-4) AC

sc-133192 AC
500 µg/ml, 25% agarose
CNY3189.00

TNF alpha 抗体 (C-4) HRP

sc-133192 HRP
200 µg/ml
CNY2422.00

TNF alpha 抗体 (C-4) FITC

sc-133192 FITC
200 µg/ml
CNY2527.00

TNF alpha 抗体 (C-4) PE

sc-133192 PE
200 µg/ml
CNY2625.00

TNF alpha 抗体 (C-4) Alexa Fluor® 488

sc-133192 AF488
200 µg/ml
CNY2738.00

TNF alpha 抗体 (C-4) Alexa Fluor® 546

sc-133192 AF546
200 µg/ml
CNY2738.00

TNF alpha 抗体 (C-4) Alexa Fluor® 594

sc-133192 AF594
200 µg/ml
CNY2738.00

TNF alpha 抗体 (C-4) Alexa Fluor® 647

sc-133192 AF647
200 µg/ml
CNY2738.00

TNF alpha 抗体 (C-4) Alexa Fluor® 680

sc-133192 AF680
200 µg/ml
CNY2738.00

TNF alpha 抗体 (C-4) Alexa Fluor® 790

sc-133192 AF790
200 µg/ml
CNY2738.00

what's the difference between sc-133192 and sc-52746? Are they all applicable for IF staining of rat liver tissue?

Asked by: Chuman
Thank you for your question. sc-133192 is raised against amino acids 77-233 of TNFα of human origin. sc-133192 is recommended for detection of TNFα of human origin by WB, IP, IF and ELISA. sc-52746 is raised against purified full length native TNFα of human origin. sc-52746 is recommended for detection of natural and recombinant TNFα of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and Guinea pig, Chimpanzee, Cynomolgus and Rhesus monkey; non cross-reactive with TNFβ or lymphotoxin.
Answered by: Ling Chen
Date published: 2025-04-21

Is a blocking peptide for this product available?

Asked by: dragonfly
Thank you for your question. This mouse monoclonal antibody, TNFα (C-4): sc-133192 is not raised against a peptide so no blocking peptide is available. The antibody is raised against amino acids 77-233 of TNFα of human origin.
Answered by: Technical Support
Date published: 2025-01-15

What is the recommended starting dilution for Western blot for TNFα (C-4): sc-133192?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-133192.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2025-11-10
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Rated 5 out of 5 by tomo12 from good for WBI can detect tnfa by WB. I used macrophage (RAW264.7) and macrophage CM.
Date published: 2018-07-13
Rated 2 out of 5 by cramos from Didn't detect any TNF in my blotsI am not sure if the antibody worked or not because I was not able to detect any TNF in my western blots and did not run a positive control.
Date published: 2018-03-13
Rated 4 out of 5 by gwang from sc-133192We used it to detect brain samples. A clear band at the correct size. There are two more prominent bands at other sizes, too
Date published: 2017-10-03
Rated 4 out of 5 by Tsen from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by wangbei from sc-133192we use it on Caco cells using WB,. Work well.
Date published: 2017-08-21
Rated 3 out of 5 by 赵忠芳12 from NOT VERY GOODTNF-A is not good to IHC,but other use not to do it .
Date published: 2017-07-21
Rated 3 out of 5 by cui sunnny from band is fuzzythe antibody samples arrived soon, but the protein band is fuzzy
Date published: 2017-07-18
Rated 3 out of 5 by fatulous from applicate it into IHCwe've applicated this antibody into the experiment of IHC , but the result is not we want because we got the positive results but in the published papers they are the negative results
Date published: 2017-05-31
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TNF alpha 抗体 (C-4) is rated 4.1 out of 5 by 17.
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