Date published: 2025-9-6
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TNF-R2 Antibody (D-2) - Immunohistochemistry - Image 284907
TNF-R2 抗体 (D-2): sc-8041. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色, 显示肾小管细胞的胞浆染色.
TNF-R2 Antibody (D-2) - Immunohistochemistry - Image 284907
TNF-R2 抗体 (D-2): sc-8041. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色, 显示肾小管细胞的胞浆染色.
TNF-R2 Antibody (D-2) - Western Blotting - Image 2856
TNF-R2 抗体 (D-2): sc-8041. SK-BR-3 (A) 和 Jurkat (B) 全细胞裂解液中 TNF-R2 p75 表达的 Western 印迹分析.
TNF-R2 Antibody (D-2) - Western Blotting - Image 391042
TNF-R2 抗体 (D-2) Alexa Fluor® 790: sc-8041 AF790. 大鼠肾脏组织提取物中 TNF-R2 表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.
TNF-R2 抗体 (D-2): sc-8041. 福尔马林固定, 石蜡包埋的人肾组织的免疫过氧化物酶染色, 显示肾小管细胞的胞浆染色.

TNF-R2 抗体 (D-2): sc-8041

4.6(13)
寫評論提問

说明书
  • TNF-R2 抗体 D-2 是小鼠单克隆 IgG2a，TNF-R2抗体, 在27篇文献中引用，规格为200 µg/ml
  • 免疫human来源TNF-R2位于C-terminus 的260-461氨基酸
  • TNF-R2 抗体 (D-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TNF-R2
  • 抗TNF-R2抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TNF-R2 (D-2): sc-8041的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成TNF-R2 抗体 (D-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TNF-R 抗体 包括 TNF-R2

TNF-R2抗体（D-2）是一种小鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的TNF-R2蛋白。TNF-R2抗体（D-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。TNF-R2蛋白（也称为TNFRSF1B）在介导肿瘤坏死因子（TNF）的作用方面发挥着关键作用，TNF是一种参与全身炎症和免疫反应的关键细胞因子。与广泛表达并介导大多数TNF功能的TNF-R1不同，TNF-R2主要在免疫细胞上表达，并参与促进细胞的存活、增殖和分化。这种选择性表达非常重要，因为TNF-R2在调节免疫反应方面发挥着重要作用，特别是在自身免疫性疾病和癌症方面，TNF-R2信号通路可以影响促炎和抗炎反应之间的平衡。了解TNF-R2的相互作用和信号传导机制对于开发靶向疗法至关重要，这些疗法可以操纵这些通路以获得治疗效益。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TNF-R2 抗体 (D-2) 参考文献:

  1. 获得性中耳胆脂瘤中的TNF-R2表达。  |  Vitale, RF., et al. 2011. Braz J Otorhinolaryngol. 77: 531-536. PMID: 21860983
  2. 骨质疏松性低冲击性骨折患者中TNF-α、TNF-R1和TNF-R2的比较分析。  |  Teixeira, AOM., et al. 2023. Rev Bras Ortop (Sao Paulo). 58: 495-499. PMID: 37396087
  3. TNF、TNF-R1、TNF-R2和TNF-R1/2缺陷小鼠齿状颗粒细胞中Lognormal样偏斜树突棘大小分布的维持。  |  Rößler, N., et al. 2024. J Comp Neurol. 532: e25645. PMID: 38943486
  4. p55 肿瘤坏死因子（TNF）受体的 '死亡结构域 '与 Fas/APO1 的自我结合，促使发出 TNF 和 Fas/APO1 效应的信号。  |  Boldin, MP., et al. 1995. J Biol Chem. 270: 387-91. PMID: 7529234
  5. 法斯死亡因素  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
  6. FADD是一种新型含死亡结构域的蛋白质，它与Fas的死亡结构域相互作用，启动细胞凋亡。  |  Chinnaiyan, AM., et al. 1995. Cell. 81: 505-12. PMID: 7538907
  7. RIP：一种含有死亡结构域的新型蛋白质，在酵母中与 Fas/APO-1 (CD95) 相互作用并导致细胞死亡。  |  Stanger, BZ., et al. 1995. Cell. 81: 513-23. PMID: 7538908
  8. Fas（Apo-1）和 TNF 受体中的死亡结构域基团存在于参与细胞凋亡和轴突导向的蛋白质中。  |  Hofmann, K. and Tschopp, J. 1995. FEBS Lett. 371: 321-3. PMID: 7556620
  9. FasL/TNF 死亡信号的竞争者。  |  Cleveland, JL. and Ihle, JN. 1995. Cell. 81: 479-82. PMID: 7758103
  10. TNF 受体 1 相关蛋白 TRADD 发出细胞死亡和 NF-kappa B 激活信号。  |  Hsu, H., et al. 1995. Cell. 81: 495-504. PMID: 7758105
  11. 细胞和病毒蛋白 TNF 受体超家族：激活, 成本刺激和死亡。  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429
  12. 生与死的转换器TNF 受体超家族及相关蛋白。  |  Baker, SJ. and Reddy, EP. 1996. Oncogene. 12: 1-9. PMID: 8552378

订购信息

产品名称产品编号规格价格数量收藏夹

TNF-R2 抗体 (D-2)

sc-8041
200 µg/ml
RMB2377.00

TNF-R2 抗体 (D-2) AC

sc-8041 AC
500 µg/ml, 25% agarose
RMB3129.00

TNF-R2 抗体 (D-2) HRP

sc-8041 HRP
200 µg/ml
RMB2377.00

TNF-R2 抗体 (D-2) FITC

sc-8041 FITC
200 µg/ml
RMB2482.00

TNF-R2 抗体 (D-2) PE

sc-8041 PE
200 µg/ml
RMB2580.00

TNF-R2 抗体 (D-2) Alexa Fluor® 488

sc-8041 AF488
200 µg/ml
RMB2685.00

TNF-R2 抗体 (D-2) Alexa Fluor® 546

sc-8041 AF546
200 µg/ml
RMB2685.00

TNF-R2 抗体 (D-2) Alexa Fluor® 594

sc-8041 AF594
200 µg/ml
RMB2685.00

TNF-R2 抗体 (D-2) Alexa Fluor® 647

sc-8041 AF647
200 µg/ml
RMB2685.00

TNF-R2 抗体 (D-2) Alexa Fluor® 680

sc-8041 AF680
200 µg/ml
RMB2685.00

TNF-R2 抗体 (D-2) Alexa Fluor® 790

sc-8041 AF790
200 µg/ml
RMB2685.00

Hi, I want to see that it also works with flow Cytometer.

Asked by: Suhaa
Thank you for your question. Fluorescent conjugates of TNF-R2 Antibody (D-2): sc-8041 can also be used for flow cytometry.
Answered by: Tech Support Europe
Date published: 2024-12-24

What is the recommended positive control for TNF-R2 Antibody (D-2): sc-8041?

Asked by: Trav11
Thank you for your question. For TNF-R2 Antibody (D-2): sc-8041, we have several recommended positive controls, which include: Jurkat Whole Cell Lysate (sc-2204), SK-BR-3 Cell Lysate (sc-2218), MCF7 Whole Cell Lysate (sc-2206), as well as a few others. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by from Good band of correct molecular weight observed.Good band of correct molecular weight observed via Western Blot in human cardiac fibroblasts cells.
Date published: 2022-11-01
Rated 4 out of 5 by from Very goodVery good antibody. It works in WB of whole cell lysates of human cells. 1/250 dilution.
Date published: 2020-06-10
Rated 5 out of 5 by from WB detectionI use this antibody for WB analysis rat kidney tissue, I suggest use the 5% BSA for block buffer for reduces the non specific signal and use dilution 1:400.
Date published: 2017-07-01
Rated 5 out of 5 by from Good WB on human VSMCssample ab tested on human VSMCs, and showed high levels of target protein with treatment.
Date published: 2017-05-09
Rated 5 out of 5 by from Excellent antibodyTNF-R2 Antibody (D-2) is a top notch choice for detection in a variety of research applications as indicated by the more than dozen product citations!
Date published: 2017-01-12
Rated 3 out of 5 by from Good band of correct molecular weight observedGood band of correct molecular weight observed via Western Blot using positive control. Using spinal cord and brain samples.
Date published: 2015-05-21
Rated 5 out of 5 by from Publishable IHC nuclear staining with paraffinPublishable IHC nuclear staining with paraffin-embedded human breast adenocarcinoma. -SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with human MPM cell lines. -SCBT Publication Review
Date published: 2015-05-01
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TNF-R2 抗体 (D-2) is rated 4.6 out of 5 by 13.
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