Date published: 2025-9-6
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TNF-R1 Antibody (H-5) - Western Blotting - Image 2857
TNF-R1 抗体 (H-5): sc-8436. MCF7 (A), HeLa (B) 和 U-937 (C) 全细胞裂解液中 TNF-R1 表达的 Western 印迹分析.
TNF-R1 抗体 (H-5): sc-8436. MCF7 (A), HeLa (B) 和 U-937 (C) 全细胞裂解液中 TNF-R1 表达的 Western 印迹分析.

TNF-R1 抗体 (H-5): sc-8436

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说明书
  • TNF-R1 抗体 H-5 是小鼠单克隆 IgG2b κ，TNF-R1抗体, 在246篇文献中引用，规格为200 µg/ml
  • 针对氨基酸30-301的抗原，在human起源的TNF-R1的细胞外域内进行定位
  • TNF-R1 抗体 (H-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TNF-R1
  • 抗TNF-R1抗体（H-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TNF-R1 (H-5): sc-8436的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 TNF-R1 抗体 (H-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TNF-R1 抗体 (H-5) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TNF-R 抗体 包括 TNF-R2

TNF-R1抗体（H-5）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的TNF-R1蛋白。TNF-R1单克隆抗体（H-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。TNF-R1蛋白，也称为肿瘤坏死因子受体超家族成员1A，在调节肿瘤坏死因子（TNF）的作用方面发挥着关键作用，TNF是一种参与全身炎症和免疫反应的关键细胞因子。TNF-R1主要位于大多数细胞类型的表面，TNF-R1启动的信号通路可导致细胞存活、凋亡或炎症，具体取决于细胞环境。TNF-R1传递凋亡信号的能力尤为重要，因为它有助于调节程序性细胞死亡，而程序性细胞死亡对于维持组织稳态和免疫系统功能至关重要。TNF-R1与TNF配体的相互作用可以激活各种下游信号级联，包括激活核因子κB（NF-κB），而后者对于炎症反应和细胞存活至关重要。了解TNF-R1的功能和信号传导机制对于开发针对炎症性疾病和癌症的治疗策略至关重要，因为TNF信号传导在这些疾病中经常失调。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TNF-R1 抗体 (H-5) 参考文献:

  1. 肾细胞表达的 TNF 受体 2（而非受体 1）对肾小球肾炎的发生至关重要。  |  Vielhauer, V., et al. 2005. J Clin Invest. 115: 1199-209. PMID: 15841213
  2. 1 型肿瘤坏死因子受体（TNF-R1）基因位于 12p13 带。  |  Derré, J., et al. 1991. Hum Genet. 87: 231-3. PMID: 1648547
  3. 人类 12 号和 1 号染色体上的肿瘤坏死因子受体基因 TNFR1 和 TNFR2。  |  Milatovich, A., et al. 1991. Somat Cell Mol Genet. 17: 519-23. PMID: 1662415
  4. TNFRSF1A R92Q 突变在类风湿性关节炎中很常见，但没有证据表明与该疾病有缔合作用或联系。  |  Dieudé, P., et al. 2007. Ann Rheum Dis. 66: 1113-5. PMID: 17234651
  5. 跨膜 TNF 与 TNFR1/2 之间的相互作用介导了单核细胞与 T 细胞的接触激活。  |  Rossol, M., et al. 2007. J Immunol. 179: 4239-48. PMID: 17785864
  6. ALS 患者的 TNF 和 sTNFR1/2 血浆水平。  |  Cereda, C., et al. 2008. J Neuroimmunol. 194: 123-31. PMID: 18083240
  7. 宿主细胞表面肿瘤坏死因子受体 I 的减少显示支持沙眼衣原体感染。  |  Paland, N., et al. 2008. J Biol Chem. 283: 6438-48. PMID: 18167350
  8. 亚裔印度人 TNF-α 和 TNFR1 启动子及 TNFR2 基因 3' UTR 区多态性与烟草相关口腔癌遗传易感性的关系。  |  Gupta, R., et al. 2008. Oral Oncol. 44: 455-63. PMID: 18206417
  9. TNFR1 启动子 -329G/T 多态性导致等全基因特异性抑制 TNFR1 的表达。  |  Kim, S., et al. 2008. Biochem Biophys Res Commun. 368: 395-401. PMID: 18243134
  10. TNF-R1与蛛网膜下腔出血后的脑灌注和急性缺血相关。  |  Fragata, I., et al. 2020. Neurocrit Care. 33: 679-687. PMID: 32820384
  11. 载入热敏F-127水凝胶的TNF-R1细胞纳米颗粒能增强烫伤皮肤的修复能力  |  Xi, L., et al. 2023. ACS Biomater Sci Eng. 9: 5843-5854. PMID: 37043416
  12. 骨质疏松性低冲击性骨折患者中TNF-α、TNF-R1和TNF-R2的比较分析。  |  Teixeira, AOM., et al. 2023. Rev Bras Ortop (Sao Paulo). 58: 495-499. PMID: 37396087

订购信息

产品名称产品编号规格价格数量收藏夹

TNF-R1 抗体 (H-5)

sc-8436
200 µg/ml
RMB2377.00

TNF-R1 (H-5): m-IgG Fc BP-HRP 套装

sc-528233
200 µg Ab; 10 µg BP
RMB2662.00

TNF-R1 (H-5): m-IgGκ BP-HRP 套装

sc-520559
200 µg Ab, 40 µg BP
RMB2662.00

TNF-R1 (H-5): m-IgG2b BP-HRP 套装

sc-550010
200 µg Ab; 10 µg BP
RMB2662.00

TNF-R1 抗体 (H-5) AC

sc-8436 AC
500 µg/ml, 25% agarose
RMB3129.00

TNF-R1 抗体 (H-5) HRP

sc-8436 HRP
200 µg/ml
RMB2377.00

TNF-R1 抗体 (H-5) FITC

sc-8436 FITC
200 µg/ml
RMB2482.00

TNF-R1 抗体 (H-5) PE

sc-8436 PE
200 µg/ml
RMB2580.00

TNF-R1 抗体 (H-5) Alexa Fluor® 488

sc-8436 AF488
200 µg/ml
RMB2685.00

TNF-R1 抗体 (H-5) Alexa Fluor® 546

sc-8436 AF546
200 µg/ml
RMB2685.00

TNF-R1 抗体 (H-5) Alexa Fluor® 594

sc-8436 AF594
200 µg/ml
RMB2685.00

TNF-R1 抗体 (H-5) Alexa Fluor® 647

sc-8436 AF647
200 µg/ml
RMB2685.00

TNF-R1 抗体 (H-5) Alexa Fluor® 680

sc-8436 AF680
200 µg/ml
RMB2685.00

TNF-R1 抗体 (H-5) Alexa Fluor® 790

sc-8436 AF790
200 µg/ml
RMB2685.00

Does the antibody block TNFa from binding to the receptor?

Asked by: CAGCAG
Thank you for your question. TNF-R1 Antibody (H-5): sc-8436 has only been tested in WB, IP, IF, IHC(P) and ELISA. There is no data concerning its blocking properties.
Answered by: Tech Support Europe
Date published: 2021-03-04

您好，请问这个抗体https://www.scbt.com/scbt/zh/product/tnf-r1-antibody-h-5?requestFrom=search 能否用来做冰冻切片的免疫组化实验？

Asked by: dora
感谢您的提问。TNF-R1 抗体 (H-5): sc-8436可以用于冰冻切片的免疫组化实验。更多问题，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your question. TNF-R1 antibody (H-5): sc-8436 can be used on frozen tissue in IHC application. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 14
Date published: 2017-08-07

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-22
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Rated 5 out of 5 by from Work well in coimmunoprecipitationThe sc-8436 anti-TNFR1 antibody works very well to detect immunoprecipitated TNFR1 from HEK293 cell lysates for ligand-receptor pull-down. The antibody works well with 1ug/ml of lysates with 1:1000 to 1:2000 dilution.
Date published: 2021-03-26
Rated 5 out of 5 by from Works for TNFR1-TRADD pull-down assayAnti-TNFR1 antibody (sc-8436) works for receptor-TRADD pull-down assay at 1:1000 concentration.
Date published: 2021-03-26
Rated 5 out of 5 by from Good bands in vascular endothelial cellsThere were good and clear bands with MW ~55 kD for vascular endothelial cell samples
Date published: 2021-01-25
Rated 5 out of 5 by from Very good antibodyWe bought and used this antibody in WB on total lysates from EA.hy926 endothelial cells
Date published: 2019-01-31
Rated 5 out of 5 by from Anti-TNFR1 AntibodyMouse anti-TNFR1 antibody (sc-8436) works great on immunoblotting at 1:1000 in 2.5 % milk powder and can be reused. It works well on IP at 1 ug for 1 ml of cell lysate.
Date published: 2017-08-24
Rated 5 out of 5 by from worked very wellOften use this antibody, strong bands in WB experiments
Date published: 2017-08-23
Rated 4 out of 5 by from sc-8436sc-8436, it works very well in WB , Caco cells .
Date published: 2017-08-21
Rated 5 out of 5 by from work well in WB 1:500 using mouse brain homegeneApplication: Western blot Gel Running Conditions:Reduced Denaturing (10% gradient gel) Specification Intestinal epithelial cell Blocking step: 5% no-fat milk as blocking agent for 1 hour(25°C) Primary antibody incubation time: 12 hours(4°C) Bands Specific: 55 kDa Additional bands at : 43 kDa (possible non-specific binding),
Date published: 2017-08-21
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TNF-R1 抗体 (H-5) is rated 4.9 out of 5 by 34.
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