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TLR8 Antibody (D-8) - Immunofluorescence - Image 151136
TLR8 抗体 (D-8): sc-373760. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.
TLR8 抗体 (D-8): sc-373760. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.

TLR8 抗体 (D-8): sc-373760

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说明书
  • TLR8 抗体 D-8 是小鼠单克隆 IgG2b κ，TLR8抗体, 在3篇文献中引用，规格为200 µg/ml
  • 免疫human物种的TLR8的氨基酸27-140
  • TLR8 抗体 (D-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 TLR8
  • 抗TLR8抗体（D-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TLR8 (D-8): sc-373760的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成TLR8 抗体 (D-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 TLR 抗体 包括 TLR1, TLR10, TLR2, TLR3, TLR4, TLR5, TLR7, TLR8和TLR9

TLR8抗体（D-8）是一种IgG2bκ小鼠单克隆TLR8抗体（也称为Toll样受体8抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的TLR8蛋白。TLR8抗体（D-8）既可作为非偶联抗TLR8抗体形式提供，也可作为多种偶联形式的抗TLR8抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Toll样受体（TLR）是一类与果蝇Toll受体具有同源性的受体，后者参与果蝇胚胎发育过程中的背腹极化，并参与宿主免疫。TLR家族成员的特征是高度保守的Toll同源（TH）结构域，这对于Toll诱导的信号转导至关重要。TLRs是I型跨膜受体，含有一个由几个富含亮氨酸的区域和单个细胞质Toll/IL-1R样结构域组成的细胞外结构域。TLR家族的三个成员，TLR7、TLR8和TLR9，属于一个差异表达的TLR亚家族。TLR7在肺、胎盘和脾脏中表达。TLR8在肺和外周血白细胞中表达，而TLR9主要在脾脏、淋巴结、骨髓和外周血白细胞中表达。TLR7、TLR8和TLR9刺激NFκB信号通路，表明它们在免疫反应中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TLR8 抗体 (D-8) 参考文献:

  1. 微生物脂蛋白通过收费样受体触发的宿主防御机制。  |  Brightbill, HD., et al. 1999. Science. 285: 732-6. PMID: 10426995
  2. 三种新型哺乳动物收费样受体：基因结构, 表达和进化。  |  Du, X., et al. 2000. Eur Cytokine Netw. 11: 362-71. PMID: 11022119
  3. 克隆和鉴定人类收费样受体亚家族：hTLR7, hTLR8 和 hTLR9。  |  Chuang, TH. and Ulevitch, RJ. 2000. Eur Cytokine Netw. 11: 372-8. PMID: 11022120
  4. 果蝇 Toll 和 IL-1 受体  |  Gay, NJ. and Keith, FJ. 1991. Nature. 351: 355-6. PMID: 1851964
  5. 利用 TLR7 和 TLR8 配体增强癌症免疫疗法。  |  Smits, EL., et al. 2008. Oncologist. 13: 859-75. PMID: 18701762
  6. MicroRNA21 与神经元 TLR8 的相遇：MicroRNA 在神经病理性疼痛中的非典型功能  |  Wang, ZH. and Liu, T. 2019. Neurosci Bull. 35: 949-952. PMID: 30887247
  7. 利用小分子药物靶向先天性免疫受体 TLR8。  |  Sakaniwa, K. and Shimizu, T. 2020. Acta Crystallogr D Struct Biol. 76: 621-629. PMID: 32627735
  8. 新型TLR8突变导致的TLR8/TLR7失调会引起同卵双胞胎严重的自身免疫性溶血性贫血和自身炎症。  |  Fejtkova, M., et al. 2022. Am J Hematol. 97: 338-351. PMID: 34981838
  9. CXCL4 与 TLR8 协同促进人类单核细胞中 TBK1-IRF5 的激活、表观基因组重塑和炎症反应。  |  Yang, C., et al. 2022. Nat Commun. 13: 3426. PMID: 35701499
  10. 低密度脂蛋白输送微生物小 RNA 通过巨噬细胞 TLR8 驱动动脉粥样硬化。  |  Allen, RM., et al. 2022. Nat Cell Biol. 24: 1701-1713. PMID: 36474072
  11. 在 CXCL4 和 TLR8 成本刺激过程中，TBK1 和 Caspase-8 可抑制坏死，但会激活 NLRP3 炎症小体的活化。  |  Cui, Y., et al. 2024. Cell Death Discov. 10: 511. PMID: 39715768
  12. 在结构上与果蝇 Toll 有关的人类受体家族。  |  Rock, FL., et al. 1998. Proc Natl Acad Sci U S A. 95: 588-93. PMID: 9435236

订购信息

产品名称产品编号规格价格数量收藏夹

TLR8 抗体 (D-8)

sc-373760
200 µg/ml
RMB2377.00

TLR8 抗体 (D-8) AC

sc-373760 AC
500 µg/ml, 25% agarose
RMB3129.00

TLR8 抗体 (D-8) HRP

sc-373760 HRP
200 µg/ml
RMB2377.00

TLR8 抗体 (D-8) FITC

sc-373760 FITC
200 µg/ml
RMB2482.00

TLR8 抗体 (D-8) PE

sc-373760 PE
200 µg/ml
RMB2580.00

TLR8 抗体 (D-8) Alexa Fluor® 488

sc-373760 AF488
200 µg/ml
RMB2685.00

TLR8 抗体 (D-8) Alexa Fluor® 546

sc-373760 AF546
200 µg/ml
RMB2685.00

TLR8 抗体 (D-8) Alexa Fluor® 594

sc-373760 AF594
200 µg/ml
RMB2685.00

TLR8 抗体 (D-8) Alexa Fluor® 647

sc-373760 AF647
200 µg/ml
RMB2685.00

TLR8 抗体 (D-8) Alexa Fluor® 680

sc-373760 AF680
200 µg/ml
RMB2685.00

TLR8 抗体 (D-8) Alexa Fluor® 790

sc-373760 AF790
200 µg/ml
RMB2685.00

Hello, It is described that the band of the TLR8 appears around 120 KDa. So, what are the 2 bands between 55KDa and 90KDa that appear in the examples, which in one case is described as being TLR8 too?

Asked by: De Pinho
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended fixation method for immunofluorescence staining with TLR8 (D-8): sc-373760 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 3 out of 5 by from WBI was able to detect TLR8 in 20 different rabbit tissues, including sperm, although as it shows in the datasheet I also obtained bands in non-specific sizes. The cleanest membranes were obtained when using rabbit semen samples. It also worked with bovine semen samples. Conditions: 1:300-1:450, 3% Milk/TBS-T, 1h.
Date published: 2021-11-04
Rated 2 out of 5 by from Indirect FCM on HFF cellsThis antibody was tested by indirect FCM on HFF cells and did not give a strong signal distinct from the background even at dilution 1:50
Date published: 2018-11-02
Rated 5 out of 5 by from Gute Ergebnisse ImmunfluoreszenzHabe verschiedene Zelllinien getestet und der AK hat super funktioniert.
Date published: 2017-12-15
Rated 1 out of 5 by from IHC Assay Review for TLR8 mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: Low, nonspecific stain at dilution factor of 10
Date published: 2017-08-31
Rated 5 out of 5 by from Single strong bands detected in human recombinantSingle strong bands detected in human recombinant TLR8. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, membrane staining in MeOH fixed HeLa cells. -SCBT QC
Date published: 2014-11-11
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TLR8 抗体 (D-8) is rated 3.5 out of 5 by 6.
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