Date published: 2026-7-21

1-800-457-3801

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.
Cas9n Nickase gRNA Plasmid Targeting: Dual gRNA plasmids create single-strand nicks at precise DNA sequences for efficient genome editing using Cas9n Nickase.
Cas9n Nickase gRNA Plasmid Targeting: Dual gRNA plasmids create single-strand nicks at precise DNA sequences for efficient genome editing using Cas9n Nickase.
This image illustrates the Cas9n Nickase mechanism used for precise genome editing. Two plasmids (Plasmid 1 and Plasmid 2) are shown, each containing a targeted DNA sequence. The system utilizes single-guide RNAs (sgRNA) to direct Cas9n Nickase to specific genomic locations, represented by the blue and pink DNA strands. The sgRNA scaffold aids in guiding Cas9n to the 20 nucleotide (nt) target sequence on the DNA. Cas9n makes single-strand cuts at NCC and NGG sites, enabling precise gene modifications without creating double-strand breaks.
This image illustrates the Cas9n Nickase mechanism used for precise genome editing. Two plasmids (Plasmid 1 and Plasmid 2) are shown, each containing a targeted DNA sequence. The system utilizes single-guide RNAs (sgRNA) to direct Cas9n Nickase to specific genomic locations, represented by the blue and pink DNA strands. The sgRNA scaffold aids in guiding Cas9n to the 20 nucleotide (nt) target sequence on the DNA. Cas9n makes single-strand cuts at NCC and NGG sites, enabling precise gene modifications without creating double-strand breaks.
The Double Nickase Plasmid features a U6 promoter for sgRNA expression, a 20 nt targeting sequence, and a gRNA scaffold to guide Cas9n. It includes a CBh promoter for Cas9n (D10A) and puromycin resistance, GFP for transfection verification, and nuclear localization signals (NLS). The 2A peptide allows co-expression of Cas9n and Puro from a single promoter, enabling precise genome editing with reduced off-target effects.

TIM-3双切口酶质粒(h): sc-416365-NIC

0.0(0)
寫評論提問

说明书
  • 针对种属：human
  • 20 µg 纯化的即用型的质粒DNA，最多可供20次转染
  • TIM-3 双切口酶质粒（h）含有一对质粒，分别编码D10A突变的Cas9核酸酶，和目标特异的 20 nt 向导RNA （gRNA），与其相应的/%base_sku_name%/ CRISPR/Cas9敲除质粒相比，它在基因敲除方面具有更好的特异性
  • 成对的向导RNA序列与目标基因中约20个碱基对互补，从而使Cas9可以模仿DNA双链断裂（DSB），介导特异的基因组DNA双切割
  • 质粒对中的一个包含供筛选使用的嘌呤霉素抗性基因；另一个包含供观察转染使用的绿色荧光蛋白标记
  • TIM-3双切酶质粒（h）和TIM-3双切酶质粒（h2）编码针对HAVCR2的不同配对gRNA设计。其中一种或两种设计可能均有提供
  • 转染后，基因敲除效率可以用抗体：TIM-3: sc-517632，通过WB, IF或者IHC分析
    Gene Editing Promo Banner

    订购信息

    产品名称产品编号规格价格数量收藏夹

    TIM-3双切口酶质粒(h)

    sc-416365-NIC
    20 µg
    		CNY3084.00

    TIM-3双切口酶质粒(h2)

    sc-416365-NIC-2
    20 µg
    		CNY3084.00

    HAVCR2 编码人T细胞免疫球蛋白与黏蛋白结构域含量蛋白3（TIM-3）。TIM-3 是一种免疫调节受体，表达于活化T细胞、调节性T细胞、NK细胞以及髓系细胞群体。TIM-3 可与半乳糖凝集素-9（galectin-9）、磷脂酰丝氨酸（phosphatidylserine）、CEACAM1 和 HMGB1 等配体结合，从而调控T细胞受体信号传导、免疫突触功能、细胞因子产生，以及与耗竭和免疫耐受相关的细胞命运决策。通过与免疫检查点网络及炎症信号程序的串扰，TIM-3 在慢性免疫刺激背景下影响抗原呈递与效应反应。HAVCR2/TIM-3 活性的失调与肿瘤免疫学、慢性病毒感染以及自身免疫性炎症中的免疫稳态改变有关，因此是开展免疫调控机制研究的一个有价值节点。

    TIM-3 双切酶质粒（h）由一对匹配的质粒组成，专为在 human 细胞系中对 HAVCR2 位点进行高特异性编辑而设计。每个质粒分别表达Cas9 D10A切口酶和针对HAVCR2内不同DNA链的独特sgRNA。当这两种切口酶被引导至相邻但位于DNA链相反侧的位点时，会产生错位的单链切口，从而共同形成错位双链断裂，这需要两个引导RNA在靶位点上协同发挥作用。由此产生的DNA断裂通过内源性细胞修复途径（最常见的是非同源末端连接（NHEJ））得到修复，从而导致插入或缺失，进而破坏HAVCR2的功能。通过要求双sgRNA在靶位点结合，双切口方法提高了编辑特异性，并为需要对靶向精度进行额外控制的应用提供了互补的CRISPR策略。

    为高效识别编辑后的细胞，其中一个质粒编码GFP以实现转染细胞群的荧光可视化，而配套质粒则携带嘌呤霉素抗性基因用于抗生素筛选。这些特性共同支持共转染细胞群的高效富集，并简化了HAVCR2基因失活克隆的验证流程。

    仅供研究使用。不用于诊断或治疗。

    1-800-457-3801
    访问 Santa Cruz Animal Health|访问 San Juan Ranch
    Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打1-800-457-3801联系我们。
    版权所有© 2007-2026 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。