Date published: 2025-9-6
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TIF1α Antibody (C-4) - Western Blotting - Image 359928
TIF1α 抗体 (C-4): sc-271266. IMR-32 (A), Hep G2 (B), COLO 205 (C), Sol8 (D) 和 F9 (E) 全细胞裂解液中 TIF1α 表达的 Western 印迹分析.
TIF1α 抗体 (C-4): sc-271266. IMR-32 (A), Hep G2 (B), COLO 205 (C), Sol8 (D) 和 F9 (E) 全细胞裂解液中 TIF1α 表达的 Western 印迹分析.

TIF1α 抗体 (C-4): sc-271266

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说明书
  • TIF1 alpha 抗体 C-4 是小鼠单克隆 IgG1 κ，TIF1α抗体, 在13篇文献中引用，规格为200 µg/ml
  • 针对氨基酸631-820的图谱，在TIF1α的内部区域，human起源
  • TIF1 alpha 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TIF1α long and short isoforms
  • 抗TIF1 alpha抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271266 X, 200 µg/0.1 ml)
  • 关于如何获取TIF1α (C-4): sc-271266的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是TIF1α Antibody (C-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与TIF1α Antibody (C-4) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外TIF1抗体，包括TIF1 α, TIF1 β和TIF1 γ

TIF1α抗体（C-4）是一种IgG1 κ小鼠单克隆TIF1 alpha抗体（也称为TIF1α抗体），可通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的TIF1 alpha蛋白。TIF1α抗体（C-4）既可作为非偶联抗TIF1 alpha抗体形式，也可作为多种偶联形式的抗TIF1 alpha抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。TIF1a通过与几种核受体的AF2区域相互作用来介导转录事件，如雌激素、维甲酸和维生素D3受体。TIF1α定位于核体，被认为与染色质和异染色质相关因子有关。TIF1α是三联基序（TRIM）家族的成员。TRIM基序包括三个锌结合域（RING、B-box类型1和B-box类型2）和一个卷曲螺旋区域。TIF1a基因映射到人类染色体7q34上，编码两种选择性剪接的转录本。然而，其中一种变体的全长性质尚未确定。在果蝇中已经鉴定出一种TIF1α同源物（称为bonus），它与几个参与蜕皮激素途径的基因相关，蜕皮激素途径是果蝇发育过程中所需的核激素受体途径，这表明该蛋白在进化过程中具有保守的功能作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TIF1α 抗体 (C-4) 参考文献:

  1. 儿童甲状腺乳头状癌中的转录辅激活因子HTIF1和一种相关蛋白与RET受体酪氨酸激酶融合。  |  Klugbauer, S. and Rabes, HM. 1999. Oncogene. 18: 4388-93. PMID: 10439047
  2. 与异染色质蛋白 1（HP1）家族成员的相互作用以及组蛋白去乙酰化在 TIF1 家族成员的转录沉默中起着不同的作用。  |  Nielsen, AL., et al. 1999. EMBO J. 18: 6385-95. PMID: 10562550
  3. Bonus 是果蝇 TIF1 蛋白的同源物，它与核受体相互作用，并能抑制 betaFTZ-F1 依赖性转录。  |  Beckstead, R., et al. 2001. Mol Cell. 7: 753-65. PMID: 11336699
  4. TIF1α在小鼠合子中作为胚胎转录调节剂的作用。  |  Torres-Padilla, ME. and Zernicka-Goetz, M. 2006. J Cell Biol. 174: 329-38. PMID: 16880268
  5. Trim24（Tif1α）基因功能的丧失使维甲酸受体α具有致癌活性。  |  Khetchoumian, K., et al. 2007. Nat Genet. 39: 1500-6. PMID: 18026104
  6. 缺乏TIF1α的小鼠出现动脉钙化和维生素D受体靶标表达增加。  |  Ignat, M., et al. 2008. Proc Natl Acad Sci U S A. 105: 2598-603. PMID: 18287084
  7. 三联体24（Trim24/Tif1α）肿瘤抑制蛋白是一种新型的负调控因子，通过抑制视黄酸受体α（Rarα）来影响干扰素（IFN）/信号转导和转录激活因子（STAT）信号通路。  |  Tisserand, J., et al. 2011. J Biol Chem. 286: 33369-79. PMID: 21768647
  8. 腺病毒 E4-ORF3 依赖于 TIF1α 和 TIF1γ 的重新定位，这有赖于对盘绕线圈基团的访问。  |  Vink, EI., et al. 2012. Virology. 422: 317-25. PMID: 22123502
  9. TIF1α相关的TRIM辅助因子将染色质修饰与转录调控、信号传导和肿瘤抑制联系起来。  |  Herquel, B., et al. 2011. Transcription. 2: 231-6. PMID: 22231120
  10. T细胞固有Tif1α/Trim24调节TH2细胞和TH2细胞介导的气道过敏的IL-1R表达。  |  Perez-Lloret, J., et al. 2016. Proc Natl Acad Sci U S A. 113: E568-76. PMID: 26787865
  11. 推测的辅助因子 TIF1alpha 是一种蛋白激酶，在与配体核受体相互作用时会过度磷酸化。  |  Fraser, RA., et al. 1998. J Biol Chem. 273: 16199-204. PMID: 9632676
  12. TIF1alpha：KRAB锌指蛋白与核受体之间可能存在的联系。  |  Le Douarin, B., et al. 1998. J Steroid Biochem Mol Biol. 65: 43-50. PMID: 9699856

订购信息

产品名称产品编号规格价格数量收藏夹

TIF1α 抗体 (C-4)

sc-271266
200 µg/ml
RMB2377.00

TIF1α (C-4): m-IgG Fc BP-HRP 套装

sc-529151
200 µg Ab; 10 µg BP
RMB2662.00

TIF1α (C-4): m-IgGκ BP-HRP 套装

sc-521895
200 µg Ab, 40 µg BP
RMB2662.00

TIF1α (C-4): m-IgG1 BP-HRP 套装

sc-543355
200 µg Ab; 20 µg BP
RMB2662.00

TIF1α 抗体 (C-4) X

sc-271266 X
200 µg/0.1 ml
RMB2377.00

TIF1α 抗体 (C-4) AC

sc-271266 AC
500 µg/ml, 25% agarose
RMB3129.00

TIF1α 抗体 (C-4) HRP

sc-271266 HRP
200 µg/ml
RMB2377.00

TIF1α 抗体 (C-4) FITC

sc-271266 FITC
200 µg/ml
RMB2482.00

TIF1α 抗体 (C-4) PE

sc-271266 PE
200 µg/ml
RMB2580.00

TIF1α 抗体 (C-4) Alexa Fluor® 488

sc-271266 AF488
200 µg/ml
RMB2685.00

TIF1α 抗体 (C-4) Alexa Fluor® 546

sc-271266 AF546
200 µg/ml
RMB2685.00

TIF1α 抗体 (C-4) Alexa Fluor® 594

sc-271266 AF594
200 µg/ml
RMB2685.00

TIF1α 抗体 (C-4) Alexa Fluor® 647

sc-271266 AF647
200 µg/ml
RMB2685.00

TIF1α 抗体 (C-4) Alexa Fluor® 680

sc-271266 AF680
200 µg/ml
RMB2685.00

TIF1α 抗体 (C-4) Alexa Fluor® 790

sc-271266 AF790
200 µg/ml
RMB2685.00

Does it work for ChIP？

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-03-28

What secondary is best to use with TIF1α (C-4): sc-271266 monoclonal antibody?

Asked by: Anna99
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with sc-271266, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-19
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Rated 5 out of 5 by from Recommended for western blotting!It detected endogenous TRIM24 in immortalized HFFs (1:500 in 5% milk in TBS-0.1% Tween, overnight at 4C).
Date published: 2017-12-12
Rated 5 out of 5 by from Works well for WBworks very well for western - samples were from human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from an excellent antibody for both WBI am very happy with this product. It gives a very good signal for WB (1:500 dilution, 40 ug HeLa lysate)
Date published: 2017-07-09
Rated 5 out of 5 by from Superb productStrong western blot data with minimal background; great IF staining
Date published: 2017-02-02
Rated 5 out of 5 by from Works Great!This antibody works great in western blot analysis of TIF1α expression in IMR-32 whole cell lysate and HeLa nuclear extract.
Date published: 2017-01-11
Rated 5 out of 5 by from Excellent antibody!We bought this antibody a month ago and it works beautifully for both human and mouse samples to detect TIF1a.
Date published: 2016-12-17
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Satisfactory Western Blot data of TIF1 expressionSatisfactory Western Blot data of TIF1 expression in IMR-32 whole cell lysate and HeLa nuclear extract . -SCBT QC
Date published: 2013-02-21
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TIF1α 抗体 (C-4) is rated 5.0 out of 5 by 8.
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