Date published: 2025-9-7
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Tie-2 Antibody (3A5) - Western Blotting - Image 352147
Tie-2 抗体 (3A5): sc-293414. NIH/3T3 全细胞裂解液中 Tie-2 表达的 Western 印迹分析.
Tie-2 抗体 (3A5): sc-293414. NIH/3T3 全细胞裂解液中 Tie-2 表达的 Western 印迹分析.

Tie-2 抗体 (3A5): sc-293414

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说明书
  • Tie-2 抗体 3A5 是小鼠单克隆 IgG2a κ，Tie-2抗体, 在11篇文献中引用，规格为100 µg/ml
  • 针对氨基酸66-185，代表human起源的Tie-2部分重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的Tie-2 WB, IP 和 ELISA检测
  • 关于如何获取Tie-2 (3A5): sc-293414的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Tie-2 Antibody (3A5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Tie-2 Antibody (3A5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Tie抗体，包括Tie-1和Tie-2

Tie-2抗体（3A5）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的Tie-2蛋白。抗Tie-2抗体（3A5）以非结合形式提供，这对于研究受体酪氨酸激酶在各种生物过程（尤其是血管系统）中的作用至关重要。Tie-2（3A5）抗体可识别Tie-2（也称为Tek），该蛋白主要位于内皮细胞表面，在血管生成和血管稳定性中起着至关重要的作用。Tie-2 (3A5) 单克隆抗体可用于研究血管形成和维持的调节机制，因为 Tie-2 介导的信号通路会影响内皮细胞的存活、增殖和迁移。 Tie-2 信号的失调与多种病理状况有关，包括癌症和心血管疾病，因此 Tie-2 成为重要的治疗干预靶点。抗Tie-2抗体（3A5）有助于研究人员研究Tie-2与多种配体的相互作用，包括对促进血管完整性和重塑至关重要的血管生成素。Tie-2（3A5）单克隆抗体为血管发育机制和Tie-2在疾病状态下的潜在影响提供了宝贵的见解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tie-2 抗体 (3A5) 参考文献:

  1. fms 样酪氨酸激酶，血管内皮生长因子的受体。  |  de Vries, C., et al. 1992. Science. 255: 989-91. PMID: 1312256
  2. 一种具有细胞外表皮生长因子同源结构域的新型内皮细胞表面受体酪氨酸激酶。  |  Partanen, J., et al. 1992. Mol Cell Biol. 12: 1698-707. PMID: 1312667
  3. tek是位于小鼠第4号染色体上的一种新型酪氨酸激酶基因，可在内皮细胞及其假定前体中表达。  |  Dumont, DJ., et al. 1992. Oncogene. 7: 1471-80. PMID: 1630810
  4. Ang-1、-2、Tie-2和VEGF在大鼠血管生成中内皮细胞和周细胞交错的定位。  |  Wakui, S., et al. 2006. Lab Invest. 86: 1172-84. PMID: 16969369
  5. 受体酪氨酸激酶：果蝇和小鼠发育过程中作用的遗传学证据。  |  Pawson, T. and Bernstein, A. 1990. Trends Genet. 6: 350-6. PMID: 1965067
  6. 在患有先兆子痫的妊娠中，血管生成素（Ang）-1、Ang-2及其受体Tie-2的胎盘mRNA表达发生了变化。  |  Kappou, D., et al. 2014. Placenta. 35: 718-23. PMID: 25047691
  7. T11TS抑制血管生成素-1/Tie-2信号传导、表皮生长因子受体激活和Raf/MEK/ERK通路，从而抑制脑内皮细胞中的血管生成，从而抑制胶质瘤模型中的血管生成。  |  Bhattacharya, D., et al. 2015. Exp Mol Pathol. 98: 455-66. PMID: 25797371
  8. 在表达 Tie-2 的细胞中缺乏 FADD 会导致 RIPK3 介导的胚胎致死。  |  Fan, C., et al. 2016. Cell Death Dis. 7: e2351. PMID: 27584790
  9. 透明细胞肾癌肿瘤血管中B7-H3和Tie-2的表达和意义。  |  Zhang, X., et al. 2017. Onco Targets Ther. 10: 5417-5424. PMID: 29180874
  10. 高氧诱导的S1P1信号通过抑制TIE-2减少血管生成，导致实验性支气管肺发育不良。  |  Sudhadevi, T., et al. 2021. Cell Biochem Biophys. 79: 561-573. PMID: 34176100
  11. 内皮特异性受体酪氨酸激酶 tek 是一个新的受体亚家族成员。  |  Dumont, DJ., et al. 1993. Oncogene. 8: 1293-301. PMID: 8386827
  12. Tie-1和tie-2定义了在早期胚胎血管系统中表达的另一类假定受体酪氨酸激酶基因。  |  Sato, TN., et al. 1993. Proc Natl Acad Sci U S A. 90: 9355-8. PMID: 8415706

订购信息

产品名称产品编号规格价格数量收藏夹

Tie-2 抗体 (3A5)

sc-293414
100 µg/ml
RMB2377.00

Tie-2 (3A5): m-IgG Fc BP-HRP 套装

sc-537744
100 µg Ab; 10 µg BP
RMB2662.00

Tie-2 (3A5): m-IgGκ BP-HRP 套装

sc-535110
100 µg Ab; 40 µg BP
RMB2662.00

Tie-2 (3A5): m-IgG2a BP-HRP 套装

sc-548106
100 µg Ab; 10 µg BP
RMB2662.00

HELLO I LOOK FOR A TIE 2 ANTIBODY THAT REACTS IN EQUINE TISSUES, THIS ANTIBODY IS SERVED? HAVE YOU BEEN TESTED?

Asked by: Stefani
Thank you for your question. We have not tested this antibody in equine tissues, nor are we aware of any publications using this antibody in equine tissues. Please contact our Technical Service Department and we can help you perform sequence analysis of the Tie-2 antibody antigens against the equine Tie-2 protein to predict any potential cross-reactivity with this species.
Answered by: Technical Support
Date published: 2018-12-14

For Western Blot, is it recommended to use denatured or non-denatured conditions with Tie-2 (3A5): sc-293414 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with human dental pulp stem cell lysate in published research. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from good resultWe already got a positive result after using Tie-2 Antibody (3A5) in our lab. We think it is a good Ab.
Date published: 2018-04-30
Rated 4 out of 5 by from it's very good for WB in ovary.This antibody is good worked for ovarian tissue for WB at 1:50
Date published: 2017-07-10
Rated 3 out of 5 by from good for western blot analysisFresh liver samples were homogenized in RIPA buffer. 40 ug of total protein were subjected to 10% SDS-PAGE and transferred onto a PVDF membrane. After blocking with 5% skim milk in TBS-T, membranes were incubated overnight with TIe-2 antibodies(1:1000).
Date published: 2017-01-06
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Tie-2 抗体 (3A5) is rated 4.0 out of 5 by 4.
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