Date published: 2025-9-7

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TIA-1 抗体 (G-3): sc-166247

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说明书
  • TIA-1 抗体 G-3 是小鼠单克隆 IgG1 κ,TIA-1抗体, 在41篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的TIA-1的N-terminus附近的氨基酸37-65之间
  • TIA-1 抗体 (G-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 TIA-1
  • 抗TIA-1抗体(G-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TIA-1 (G-3): sc-166247免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRPm-IgGκ BP-HRP 是 TIA-1 抗体 (G-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TIA-1 抗体 (G-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

TIA-1抗体(G-3)是一种IgG1 κ小鼠单克隆TIA-1抗体(也称为TIA1抗体、Nucleolysin TIA-1抗体或p40-TIA-1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的TIA-1蛋白。TIA-1抗体(G-3)既可作为非偶联抗TIA-1抗体形式提供,也可作为多种偶联形式的抗TIA-1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。FAS,也被称为CD95或APO-1,是一种I型跨膜蛋白,在介导病毒免疫中起着核心作用。TIA-1和TIAR是两个密切相关的蛋白,它们拥有三个RRM(RNA识别基序),分别被命名为RRM 1、2和3。尽管TIA-1和TIAR都被认为是凋亡细胞死亡的介质,但它们在这些通路中的具体作用尚不清楚。与在细胞毒性淋巴细胞颗粒中发现的TIA-1不同,TIAR的表达仅限于细胞核,并且在更广泛的细胞中发现,包括但不限于造血起源的细胞。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TIA-1 抗体 (G-3) 参考文献:

  1. 促进细胞凋亡蛋白TIA-1调控基因表达的分子机制。  |  Förch, P. and Valcárcel, J. 2001. Apoptosis. 6: 463-8. PMID: 11595836
  2. 鉴定介导 TIAR 和 TIA-1 RNA 结合蛋白的核-细胞质穿梭的序列决定因素。  |  Zhang, T., et al. 2005. J Cell Sci. 118: 5453-63. PMID: 16278295
  3. RNA结合蛋白TIA-1, TIAR和HuR控制ATP合成酶β亚基的表达。  |  Izquierdo, JM. 2006. Biochem Biophys Res Commun. 348: 703-11. PMID: 16890199
  4. Fas 活化丝氨酸/苏氨酸激酶(FAST K)与 TIA-1/TIAR 蛋白协同调节 Fas 的替代剪接。  |  Izquierdo, JM. and Valcárcel, J. 2007. J Biol Chem. 282: 1539-43. PMID: 17135269
  5. 人类结肠癌中 TIA-1 的替代剪接调节血管内皮生长因子同工酶的表达, 血管生成, 肿瘤生长和贝伐珠单抗的耐药性。  |  Hamdollah Zadeh, MA., et al. 2015. Mol Oncol. 9: 167-78. PMID: 25224594
  6. TIA-1、MFF、microRNA-200a-3p和microRNA-27作为肝癌新型标志物的潜在用途。  |  Tak, H., et al. 2018. Biochem Biophys Res Commun. 497: 1117-1122. PMID: 29496454
  7. Fas 及其配体在 T 细胞介导的细胞毒性的一般机制中的作用。  |  Hanabuchi, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 4930-4. PMID: 7515183
  8. 在 Fas 介导的细胞凋亡过程中,RNA 结合蛋白 TIAR 从细胞核转位到细胞质。  |  Taupin, JL., et al. 1995. Proc Natl Acad Sci U S A. 92: 1629-33. PMID: 7533298
  9. 在 Fas 介导的细胞凋亡过程中,Fas 激活的丝氨酸/苏氨酸激酶(FAST)将 TIA-1 磷酸化。  |  Tian, Q., et al. 1995. J Exp Med. 182: 865-74. PMID: 7544399
  10. 已丧失 MHC 非限制性细胞毒性活性,但在 CD3 受体刺激下仍能保持重定向杀伤力的 TCR γ δ + 细胞克隆。  |  Visonneau, S., et al. 1995. Cell Immunol. 165: 252-65. PMID: 7553890
  11. TIA-1:一类新型细胞溶解效应分子的结构和功能研究。  |  Anderson, P. 1995. Curr Top Microbiol Immunol. 198: 131-43. PMID: 7774278
  12. TIA-1 和 TIAR 的各个 RNA 识别基团具有不同的 RNA 结合特异性。  |  Dember, LM., et al. 1996. J Biol Chem. 271: 2783-8. PMID: 8576255

订购信息

产品名称产品编号规格价格数量收藏夹

TIA-1 抗体 (G-3)

sc-166247
200 µg/ml
RMB2377.00

TIA-1 (G-3): m-IgGκ BP-HRP 套装

sc-521543
200 µg Ab, 40 µg BP
RMB2662.00

TIA-1 (G-3): m-IgG1 BP-HRP 套装

sc-533497
200 µg Ab; 20 µg BP
RMB2662.00

TIA-1 抗体 (G-3) AC

sc-166247 AC
500 µg/ml, 25% agarose
RMB3129.00

TIA-1 抗体 (G-3) HRP

sc-166247 HRP
200 µg/ml
RMB2377.00

TIA-1 抗体 (G-3) FITC

sc-166247 FITC
200 µg/ml
RMB2482.00

TIA-1 抗体 (G-3) PE

sc-166247 PE
200 µg/ml
RMB2580.00

TIA-1 抗体 (G-3) Alexa Fluor® 488

sc-166247 AF488
200 µg/ml
RMB2685.00

TIA-1 抗体 (G-3) Alexa Fluor® 546

sc-166247 AF546
200 µg/ml
RMB2685.00

TIA-1 抗体 (G-3) Alexa Fluor® 594

sc-166247 AF594
200 µg/ml
RMB2685.00

TIA-1 抗体 (G-3) Alexa Fluor® 647

sc-166247 AF647
200 µg/ml
RMB2685.00

TIA-1 抗体 (G-3) Alexa Fluor® 680

sc-166247 AF680
200 µg/ml
RMB2685.00

TIA-1 抗体 (G-3) Alexa Fluor® 790

sc-166247 AF790
200 µg/ml
RMB2685.00

TIA-1 (G-3) 中和勝肽

sc-166247 P
100 µg/0.5 ml
RMB511.00

Does the Anti-TIA-1 Antibody (G-3) bind NKG7? Has anyone used the Anti-TIA-1 Antibody (G-3) PE sc-166247 PE for flow cytometry?

Asked by: DannyP
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hi, we want to use this TIA-1 antibody to do IF staining on mouse brain tissue. Could you please give me some information about the dilution ratio of the primary antibody and secondary antibody? what secondary antibody did you use? Thanks! Jianjun

Asked by: Jianjun
Thank you for your question. We recommend a starting dilution of 1:50 for this TIA-1 antibody for immunofluorescence use. You can find the recommended protocol here: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining You can use any anti-mouse secondary antibody. We highly recommend using our optimized mouse IgG binding proteins, you can find them here: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2020-01-27

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 10
Date published: 2017-01-19
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Rated 3 out of 5 by from Low signal in XenopusI tried this antibody for IF in Xenopus RGC axons with a 1:100 dilution. Low signal but specific.
Date published: 2021-10-07
Rated 4 out of 5 by from Great antibody for both WB and IF!I used this antibody for IF and WB in HT1080 cells, and worked very well!
Date published: 2019-03-18
Rated 5 out of 5 by from It is good~~Thought it detected two band in our cell lines,but when using siRNA to knock it down, one band disappear thoroughly. This antibody surly can be used for TIA1 detection
Date published: 2018-02-19
Rated 5 out of 5 by from it works for western blotThis antibody works well for western blot. We tested in PANC-1 and MD-MBA-231
Date published: 2018-02-06
Rated 1 out of 5 by from This antibody does not work for ICC or IFI tried this sample antibody for ICC and IF using HEK239T cells and mouse brain sections. I tried different dilutions (1:50-1:500) and different block buffers (milk, serum and commercial block buffers). Unfortunately, I did not get any specific staining.
Date published: 2018-01-02
Rated 5 out of 5 by from Wow !! Superb productOutstanding for multiple applications including WB, IF and IHC staining
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of TIA-1 expression in BJAB, Jurkat and K-562 whole cell lysates.
Date published: 2017-01-12
Rated 2 out of 5 by from Tried in IHC for ASTMATried in IHC for ASTMA-23 & Lymph TMA. NSS -Not good Specificity
Date published: 2016-06-13
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TIA-1 抗体 (G-3) is rated 4.2 out of 5 by 13.
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