Date published: 2025-9-8
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Thymidine Kinase Antibody (C-4) - Western Blotting - Image 358019
胸苷激酶抗体 (C-4): sc-377211. K-562 (A) 和 HL-60 (B) 全细胞裂解液中胸苷激酶表达的 Western 印迹分析.
Thymidine Kinase Antibody (C-4) - Western Blotting - Image 358019
胸苷激酶抗体 (C-4): sc-377211. K-562 (A) 和 HL-60 (B) 全细胞裂解液中胸苷激酶表达的 Western 印迹分析.
Thymidine Kinase Antibody (C-4) - Western Blotting - Image 282536
胸苷激酶抗体 (C-4): sc-377211. K-562 (A) 和 AML-193 (B) 全细胞裂解液中胸苷激酶表达的 Western 印迹分析.
Thymidine Kinase Antibody (C-4) - Western Blotting - Image 364032
胸苷激酶抗体 (C-4): sc-377211. K-562 (A), THP-1 (B) 和 TF-1 (C) 全细胞裂解液中胸苷激酶表达的 Western 印迹分析.
胸苷激酶抗体 (C-4): sc-377211. K-562 (A) 和 HL-60 (B) 全细胞裂解液中胸苷激酶表达的 Western 印迹分析.

Thymidine Kinase 抗体 (C-4): sc-377211

4.2(5)
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说明书
  • Thymidine Kinase 抗体 C-4 是小鼠单克隆 IgG1 κ，Thymidine Kinase抗体, 在3篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长Thymidine Kinase的氨基酸1-234
  • Thymidine Kinase 抗体 (C-4) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Thymidine Kinase
  • 抗Thymidine Kinase抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Thymidine Kinase (C-4): sc-377211的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Thymidine Kinase Antibody (C-4) for WB applications. 的首选辅助检测试剂，这些试剂现与Thymidine Kinase Antibody (C-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

胸腺嘧啶激酶抗体（C-4）是一种IgG1 κ小鼠单克隆胸腺嘧啶激酶抗体（也称为胸腺嘧啶激酶抗体），它能够通过WB、IP、IF和ELISA等方法检测人源胸腺嘧啶激酶蛋白。胸腺嘧啶激酶抗体（C-4）以非偶联的抗胸腺嘧啶激酶抗体形式提供，同时也有多种偶联形式的抗胸腺嘧啶激酶抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胸腺嘧啶激酶（TK1）是一种存在于大多数活细胞中的高度保守的磷酸转移酶。胸腺嘧啶激酶催化磷酸化反应：脱氧胸苷+ATP=脱氧胸苷5'-磷酸+ADP；因此，它参与将脱氧胸苷引入DNA的反应链。胸腺嘧啶激酶是许多抗病毒药物（如叠氮胸苷（AZT））发挥作用所必需的，同时也用于在单克隆抗体生产过程中选择杂交瘤细胞系。胸腺嘧啶激酶具有许多临床应用，因为它仅在预期细胞分裂时才出现。因此，胸腺嘧啶激酶可作为增殖标志物，用于恶性肿瘤，尤其是血液系统恶性肿瘤的诊断、治疗和随访。胸腺嘧啶激酶可以单体、二聚体、三聚体或四聚体的形式存在。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Thymidine Kinase 抗体 (C-4) 参考文献:

  1. 用表达单纯疱疹病毒胸苷激酶的复制腺病毒载体治疗恶性胶质瘤。  |  Nanda, D., et al. 2001. Cancer Res. 61: 8743-50. PMID: 11751394
  2. 线粒体胸苷激酶和培养的人细胞中调节三磷酸胸苷池的酶网络。  |  Rampazzo, C., et al. 2007. J Biol Chem. 282: 34758-69. PMID: 17913703
  3. 溶组织阿米巴的胸苷激酶和尿苷-胞苷激酶：克隆、表征和寻找特异性抑制剂。  |  Lossani, A., et al. 2009. Parasitology. 136: 595-602. PMID: 19368743
  4. 人类与黑猩猩的基因同源性：半乳糖激酶和胸苷激酶基因的同源关系以及利用黑猩猩-小鼠体细胞杂交诱导的腺病毒-12间隙。  |  Chen, S., et al. 1976. Somatic Cell Genet. 2: 205-13. PMID: 195354
  5. 胸苷激酶和半乳糖激酶基因在麝第 11 号染色体上的分配以及该染色体在中国仓鼠/小鼠体细胞杂交种中的优先分离。  |  Kozak, CA. and Ruddle, FH. 1977. Somatic Cell Genet. 3: 121-33. PMID: 204065
  6. 用 TaqI 酶检测细胞质胸苷激酶基因 TK1（17q21-q22）的频繁多态性。  |  Murphy, PD., et al. 1986. Nucleic Acids Res. 14: 4381. PMID: 3012471
  7. 人类胸苷激酶基因的序列, 结构和启动子特征。  |  Flemington, E., et al. 1987. Gene. 52: 267-77. PMID: 3301530
  8. 人类细胞质胸苷激酶。从 HeLa 细胞中纯化这种酶并确定其物理特性。  |  Sherley, JL. and Kelly, TJ. 1988. J Biol Chem. 263: 375-82. PMID: 3335503
  9. 基于纳米通道的生物传感器，用于超灵敏和无标记检测胸苷激酶活性。  |  Rauf, A., et al. 2024. Talanta. 279: 126626. PMID: 39116732
  10. 将三个人类基因分配到染色体上（LDH-A 到 11，TK 到 17，IDH 到 20），以及体细胞杂交中人类和小鼠染色体之间易位的证据（胸苷激酶-乳酸脱氢酶 A-异柠檬酸脱氢酶-C-11, E-17 和 F-20 染色体）。  |  Boone, C., et al. 1972. Proc Natl Acad Sci U S A. 69: 510-4. PMID: 4110482
  11. 腺病毒对正常人体细胞和 E 染色体三体细胞染色体的影响。  |  McDougall, JK. 1970. Nature. 225: 456-8. PMID: 5411122
  12. 人类胸苷激酶基因：可在哺乳动物细胞中表达的 cDNA 的分子克隆和核苷酸序列。  |  Bradshaw, HD. and Deininger, PL. 1984. Mol Cell Biol. 4: 2316-20. PMID: 6549046

订购信息

产品名称产品编号规格价格数量收藏夹

Thymidine Kinase 抗体 (C-4)

sc-377211
200 µg/ml
RMB2377.00

Thymidine Kinase (C-4): m-IgG Fc BP-HRP 套装

sc-527586
200 µg Ab; 10 µg BP
RMB2662.00

Thymidine Kinase (C-4): m-IgG1 BP-HRP 套装

sc-532959
200 µg Ab; 20 µg BP
RMB2662.00

Thymidine Kinase 抗体 (C-4) AC

sc-377211 AC
500 µg/ml, 25% agarose
RMB3129.00

Thymidine Kinase 抗体 (C-4) HRP

sc-377211 HRP
200 µg/ml
RMB2377.00

Thymidine Kinase 抗体 (C-4) FITC

sc-377211 FITC
200 µg/ml
RMB2482.00

Thymidine Kinase 抗体 (C-4) PE

sc-377211 PE
200 µg/ml
RMB2580.00

Thymidine Kinase 抗体 (C-4) Alexa Fluor® 488

sc-377211 AF488
200 µg/ml
RMB2685.00

Thymidine Kinase 抗体 (C-4) Alexa Fluor® 546

sc-377211 AF546
200 µg/ml
RMB2685.00

Thymidine Kinase 抗体 (C-4) Alexa Fluor® 594

sc-377211 AF594
200 µg/ml
RMB2685.00

Thymidine Kinase 抗体 (C-4) Alexa Fluor® 647

sc-377211 AF647
200 µg/ml
RMB2685.00

Thymidine Kinase 抗体 (C-4) Alexa Fluor® 680

sc-377211 AF680
200 µg/ml
RMB2685.00

Thymidine Kinase 抗体 (C-4) Alexa Fluor® 790

sc-377211 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for sc-377211: Thymidine Kinase (C-4) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from L'anticorpo funziona molto benequesto campione è stato richiesto per verificare la presenza della timidina chinasi in cellule HEK293T
Date published: 2021-05-12
Rated 2 out of 5 by from TK1 reviewIn this experiment, we want to troubleshoot a potential phenotype on the endogenous TK1 levels in presence of two differents "non-TK1 expressors". HEK-293T cells were transfected or not with non-TK1 expressors for 48h. Cells were lysed in RIPA-DOC (+ protease inhibitors) for 30min at 4c and spin 10.000rpm/5min/4c. Proteins quantification was done and 50µg of lysates were migrated on a 12% SDS-PAGE. After 4h transfer on nitrocellulose membrane, membrane were blocked with 5% non-fat milk and probe overnight with the anti-TK1 antibody (C-4 from Santa Cruz; dilution 1/500). Following the O/N incubation, membrane was washed with 1x TBS-T and probe with the corresponding secondary HRP-conjugated Ab. WB was revealed and the corresponding data are shown in the attached photo. The detection signal is very poor and we are almost not able to see the corresponding endogenous TK1 band in the mock and Cond2.
Date published: 2017-10-10
Rated 4 out of 5 by from WBI have detected three strong WB bands in THP1 cells , but there was also signal in human samples by IHC-P
Date published: 2017-02-10
Rated 5 out of 5 by from Great for Western blotBeautiful antibody for Western blot: nice strong single bands every time. Smart choice!
Date published: 2017-01-24
Rated 5 out of 5 by from Produced positive Western blot data of ThymidineProduced positive Western blot data of Thymidine Kinase expression in K-562 and AML-193 whole cell lysates. -SCBT QC
Date published: 2014-07-10
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Thymidine Kinase 抗体 (C-4) is rated 4.2 out of 5 by 5.
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