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Thy-1/CD90 Antibody (aTHy-1A1) - Western Blotting - Image 52295 — Thy-1 抗体 (aTHy-1A1): sc-53456. 小鼠 PBL 全细胞裂解液中 Thy-1 表达的 Western 印迹分析.

Thy-1/CD90 Antibody (aTHy-1A1) - Immunohistochemistry - Image 361405 — Thy-1抗体 (aTHy-1A1): sc-53456. 福尔马林固定, 石蜡包埋的小鼠脑组织的免疫过氧化物酶染色显示神经元细胞和胶质细胞的胞浆染色.

Thy-1 抗体 (aTHy-1A1): sc-53456. 小鼠 PBL 全细胞裂解液中 Thy-1 表达的 Western 印迹分析.

Thy-1/CD90 抗体 (aTHy-1A1): sc-53456

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说明书

Thy-1/CD90 抗体 aTHy-1A1 是小鼠单克隆 IgG_2a κ，Thy-1/CD90抗体, 在36篇文献中引用，规格为200 µg/ml
针对纯化的Thy-1 human 起源
Thy-1/CD90 抗体 (aTHy-1A1) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Thy-1
抗Thy-1/CD90抗体（aTHy-1A1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG_2a BP-HRP是Thy-1/CD90 Antibody (aTHy-1A1) 适用于 WB 和 IHC(P) 应用。的首选辅助检测试剂。这些试剂现与Thy-1/CD90 Antibody (aTHy-1A1) 打包提供（请参阅下面的订购信息）。

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Thy-1/CD90 抗体 (aTHy-1A1) 参考文献:

Thy-1与星形胶质细胞上的整合素β（3）结合，触发形成局灶性接触点。 | Leyton, L., et al. 2001. Curr Biol. 11: 1028-38. PMID: 11470407
Sca-1和Thy-1加速骨髓基质细胞中神经元样分化。 | Mizobe, T., et al. 2006. Kobe J Med Sci. 52: 119-25. PMID: 17006052
内源性Thy-1基因和转染小鼠Thy-1.2基因在大鼠嗜碱性白血病细胞中的功能表达。 | Dráberová, L. and Dráber, P. 1991. Eur J Immunol. 21: 1583-90. PMID: 1711974
心脏成纤维细胞中的Thy-1表达：与肌成纤维细胞收缩标记无关。 | Hudon-David, F., et al. 2007. J Mol Cell Cardiol. 42: 991-1000. PMID: 17395197
Thy-1启动子高甲基化：肺纤维化中一种新的表观遗传致病机制。 | Sanders, YY., et al. 2008. Am J Respir Cell Mol Biol. 39: 610-8. PMID: 18556592
从一组Thy-1骨髓前体细胞中独立产生Thy-1+表皮细胞。 | Honjo, M., et al. 1990. J Invest Dermatol. 95: 562-7. PMID: 1977801
黑色素瘤细胞利用内皮细胞上的Thy-1（CD90）形成转移。 | Schubert, K., et al. 2013. Am J Pathol. 182: 266-76. PMID: 23159525
人类Thy-1的GPI锚对抗体识别和功能的影响。 | Bradley, JE., et al. 2013. Lab Invest. 93: 365-74. PMID: 23358110
Wnt/β-catenin在thy-1的介导下帮助调节hepG2细胞的增殖。 | Cheng, BQ., et al. 2014. Genet Mol Res. 13: 5115-27. PMID: 25061736
转基因小鼠中Thy-1基因表达的调控。 | Gordon, JW., et al. 1987. Cell. 50: 445-52. PMID: 2886226

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	THY1	Entrez Gene ID: 7070	11q23.3	NM_001311160, NM_001311162, NM_006288	UniProt ID: P04216	188230
Mouse	Thy1	Entrez Gene ID: 21838	9 A5.1	NM_009382	UniProt ID: P01831	N/A

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
Thy-1/CD90 抗体 (aTHy-1A1)	sc-53456	200 µg/ml	CNY2422.00
Thy-1/CD90 (aTHy-1A1): m-IgG Fc BP-HRP 套装	sc-525599	200 µg Ab; 10 µg BP	CNY2715.00
Thy-1/CD90 (aTHy-1A1): m-IgG_2a BP-HRP 套装	sc-546333	200 µg Ab; 10 µg BP	CNY2715.00
Thy-1/CD90 抗体 (aTHy-1A1) AC	sc-53456 AC	500 µg/ml, 25% agarose	CNY3189.00
Thy-1/CD90 抗体 (aTHy-1A1) HRP	sc-53456 HRP	200 µg/ml	CNY2422.00
Thy-1/CD90 抗体 (aTHy-1A1) FITC	sc-53456 FITC	200 µg/ml	CNY2527.00
Thy-1/CD90 抗体 (aTHy-1A1) PE	sc-53456 PE	200 µg/ml	CNY2625.00
Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor^® 488	sc-53456 AF488	200 µg/ml	CNY2738.00
Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor^® 546	sc-53456 AF546	200 µg/ml	CNY2738.00
Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor^® 594	sc-53456 AF594	200 µg/ml	CNY2738.00
Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor^® 647	sc-53456 AF647	200 µg/ml	CNY2738.00
Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor^® 680	sc-53456 AF680	200 µg/ml	CNY2738.00
Thy-1/CD90 抗体 (aTHy-1A1) Alexa Fluor^® 790	sc-53456 AF790	200 µg/ml	CNY2738.00

请问这个抗体适用于哪些细胞株?

Asked by: ljy123

Thanks for your question. Thy-1/CD90 Antibody (aTHy-1A1) is recommended for detection of Thy-1 of mouse, rat and human origin. CTLL-2 cell lysate, IMR-32 cell lysate or CCRF-CEM cell lysate can be used as Positive Controls. 感谢您的提问。Thy-1/CD90 抗体 (aTHy-1A1) 推荐用于检测mouse, rat 和human 来源的 Thy-1。CTLL-2 cell lysate, IMR-32 cell lysate or CCRF-CEM cell lysate 可以作为阳性对照细胞系。

Answered by: Yanru Tao

Date published: 2023-05-05

Can Thy-1 (aTHy-1A1): sc-53456 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Thy-1 (aTHy-1A1): sc-53456 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by Miguel from Thy-1/CD90 AntibodyThe antibody worked perfectly fine in my experiments with mouse cells.

Date published: 2025-11-16

Rated 5 out of 5 by P Vyas from IF for human Mesenchymal Stem Cells is great!Received a sample of this antibody two months ago and it has been excellent for IF for human mesenchymal stem cells. Tried 1:50 and it was great! Definitely purchasing!

Date published: 2020-03-10

Rated 4 out of 5 by Arely G from antibody acceptableuse dilution 1: 150 over night for immunofluoresce

Date published: 2018-04-10

Rated 4 out of 5 by Yaogo from Equine dermal cells are negativeGot sample of this item and tried to use on my equine dermal cells (fibroblast cell line). The procedure: triton X-100 for permeabilization for 30 min, followed by blocking for 30 min. Then, 1:20 dilution of this Ab for 1h at room temperature, followed by standard protocol of immunohistochemical staining with ABC kit and AEC chromogen. Cells were totally negative. But these cells are strongly positive for vimentin v9.

Date published: 2017-12-10

Rated 3 out of 5 by sun J from 阴性申请了试用装，态度很好，速度很快，但是大鼠眼球切片节细胞染色为阴性，免疫荧光，1:200，不知为什么

Date published: 2017-10-09

Rated 4 out of 5 by JM from Positive signal in WB in mouse PBL wholePositive signal in WB in mouse PBL whole cell lysate. -SCBT QC

Date published: 2015-06-19

Rated 4 out of 5 by MS from Published for IHC and ELISA in human liverPublished for IHC and ELISA in human liver cancer tissue. -SCBT Publication Review

Date published: 2015-05-15

Rated 4 out of 5 by SF from Good publishable data in flow cytometry inGood publishable data in flow cytometry in human tooth germ stem cells. -SCBT Publication Review

Date published: 2014-07-18

Thy-1/CD90 抗体 (aTHy-1A1) is rated 4.1 out of 5 by 8.

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Thy-1/CD90 抗体 (aTHy-1A1): sc-53456

Thy-1/CD90 抗体 (aTHy-1A1) 参考文献:

Thy-1/CD90 抗体 (aTHy-1A1) 规格

替代产品名称: CD90

产品克隆名称: aTHy-1A1

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG_2a kappa light chain

氨基酸:

抗原表位靶标: Thy-1

抗原表位种属: human

产品反应性/检测: Thy-1

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), FCM

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 36篇各种科学出版物的引文

Thy-1/CD90 抗体 (aTHy-1A1)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 Thy-1/CD90 抗體是 Thy-1/CD90 抗體 (OX7): sc-53116.