Date published: 2025-9-7
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Thrombin Antibody (F-1) - Western Blotting - Image 355308
凝血酶抗体 (F-1): sc-271449. Hep G2 (A), AML-193 (B), FHs 173We (C), CCRF-CEM (D) 和 HL-60 (E) 全细胞裂解液中凝血酶表达的 Western 印迹分析.
凝血酶抗体 (F-1): sc-271449. Hep G2 (A), AML-193 (B), FHs 173We (C), CCRF-CEM (D) 和 HL-60 (E) 全细胞裂解液中凝血酶表达的 Western 印迹分析.

Thrombin 抗体 (F-1): sc-271449

4.6(5)
寫評論提問

说明书
  • Thrombin抗体(F-1)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Thrombin的内部区域内的氨基酸331-376之间
  • 抗-Thrombin 抗体 (F-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Prothrombin precursor and mature chain, and Thrombin LC
  • 抗Thrombin抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Thrombin (F-1): sc-271449的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是Thrombin Antibody (F-1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Thrombin Antibody (F-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Thrombin抗体，包括Thrombin API, Thrombin APII和Thrombin LC

凝血酶抗体（F-1）是一种IgG1 κ小鼠单克隆凝血酶抗体，它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的凝血酶原前体和成熟链，以及凝血酶轻链。凝血酶抗体（F-1）可作为非共轭抗凝血酶抗体形式提供，也可作为多种共轭形式的抗凝血酶抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。组织损伤后的止血涉及基本血浆促凝剂（凝血酶原和因子X、IX、V和VIII）的部署，这些促凝剂参与凝血级联反应，导致不溶性纤维蛋白凝块的形成和血小板聚集的促进。凝血因子2，也被称为凝血酶原或因子2，在凝血级联反应的第一步中被蛋白水解切割形成凝血酶。凝血酶是一种丝氨酸蛋白酶，影响细胞有丝分裂、肿瘤生长和转移，并能启动血小板聚集和分泌。凝血酶还在发育和出生后生命中影响血管完整性。在伤口愈合机制中，凝血酶可能通过刺激成纤维细胞前胶原蛋白的产生来协调结缔组织蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Thrombin 抗体 (F-1) 参考文献:

  1. 凝血酶抑制肿瘤细胞生长与 p21(waf/cip1)和 caspases 的上调有关，而 p21(waf/cip1)和 caspases 是通过 p53 依赖性和 STAT-1 依赖性途径实现的。  |  Huang, YQ., et al. 2000. J Biol Chem. 275: 6462-8. PMID: 10692450
  2. 一项关于局部重组人凝血酶和牛凝血酶在外科止血中的有效性和安全性的 3 期随机双盲比较研究。  |  Chapman, WC., et al. 2007. J Am Coll Surg. 205: 256-65. PMID: 17660072
  3. 基于掺杂 Ru(bpy)3(2+) 的二氧化硅纳米粒子的电生化学发光传感器通过靶蛋白诱导的链位移检测凝血酶。  |  Wang, X., et al. 2007. Anal Chim Acta. 598: 242-8. PMID: 17719898
  4. 酪氨酸磷酸化/去磷酸化平衡参与凝血酶诱发的人类血小板微管重组  |  Bouaziz, A., et al. 2007. Thromb Haemost. 98: 375-84. PMID: 17721620
  5. 血浆中 Xa 因子驱动的凝血酶生成：取决于膜的氨基磷脂密度和磷脂结合蛋白的抑制作用。  |  Wielders, SJ., et al. 2007. Thromb Haemost. 98: 1056-62. PMID: 18000611
  6. 凝血级联：启动, 维持和调节。  |  Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
  7. 血液凝固的基本机制  |  Davie, EW. and Fujikawa, K. 1975. Annu Rev Biochem. 44: 799-829. PMID: 237463
  8. 凝血酶和凝血酶受体激活对急性心肌梗死后心脏功能的影响。  |  Gu, X., et al. 2015. Am J Transl Res. 7: 654-69. PMID: 26064435
  9. 一种测量纤维蛋白原和凝血酶粉混合物中凝血酶活性的方法。  |  DeAnglis, AP., et al. 2017. Blood Coagul Fibrinolysis. 28: 134-138. PMID: 26991860
  10. 编码凝血酶原和脑磷脂的人类基因分别位于 11p11-q12 和 3q21-24。  |  Royle, NJ., et al. 1987. Somat Cell Mol Genet. 13: 285-92. PMID: 3474786
  11. 凝血酶对基质纤连蛋白的刺激。  |  Armstrong, MT., et al. 1996. J Cell Physiol. 166: 112-20. PMID: 8557759
  12. 凝血酶通过蛋白酶活化受体 1 的蛋白水解激活作用刺激成纤维细胞产生胶原蛋白原。  |  Chambers, RC., et al. 1998. Biochem J. 333 (Pt 1): 121-7. PMID: 9639571

订购信息

产品名称产品编号规格价格数量收藏夹

Thrombin 抗体 (F-1)

sc-271449
200 µg/ml
RMB2377.00

Thrombin (F-1): m-IgG1 BP-HRP 套装

sc-531686
200 µg Ab; 20 µg BP
RMB2662.00

Thrombin 抗体 (F-1) AC

sc-271449 AC
500 µg/ml, 25% agarose
RMB3129.00

Thrombin 抗体 (F-1) HRP

sc-271449 HRP
200 µg/ml
RMB2377.00

Thrombin 抗体 (F-1) FITC

sc-271449 FITC
200 µg/ml
RMB2482.00

Thrombin 抗体 (F-1) PE

sc-271449 PE
200 µg/ml
RMB2580.00

Thrombin 抗体 (F-1) Alexa Fluor® 488

sc-271449 AF488
200 µg/ml
RMB2685.00

Thrombin 抗体 (F-1) Alexa Fluor® 546

sc-271449 AF546
200 µg/ml
RMB2685.00

Thrombin 抗体 (F-1) Alexa Fluor® 594

sc-271449 AF594
200 µg/ml
RMB2685.00

Thrombin 抗体 (F-1) Alexa Fluor® 647

sc-271449 AF647
200 µg/ml
RMB2685.00

Thrombin 抗体 (F-1) Alexa Fluor® 680

sc-271449 AF680
200 µg/ml
RMB2685.00

Thrombin 抗体 (F-1) Alexa Fluor® 790

sc-271449 AF790
200 µg/ml
RMB2685.00

Thrombin (F-1) 中和勝肽

sc-271449 P
100 µg/0.5 ml
RMB511.00

Dear team, I would like to know the antibody antigen used. It was indicated in the information, but could you let me know the exact peptide amino acid sequences listed in the information? (sorry that I sometimes miss the numbering in the mature form)

Asked by: Antibody Epitope seq
Thank you for contacting Santa Cruz Biotechnology. The requested information is proprietary but, here is a link for more information. https://www.uniprot.org/uniprotkb/P00734/entry
Answered by: BlakeJ
Date published: 2025-04-30

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 4 out of 5 by from Good labelling!This antibody was used in paraffin-embedded mouse brain sections and only worked with a pH 9.0 antigen retrieval. The labelling obtained was strong and without background.
Date published: 2017-03-23
Rated 4 out of 5 by from Boa marcação, sem marcação inespecíficaUtilizado em amostras de cérebro embebidas em parafina; necessário fazer recuperação antigénica com tampão Tris-EDTA, pH 9.
Date published: 2017-03-08
Rated 5 out of 5 by from Very reliable and strong!This antibody excels in both WB and IHC applications. Nice strong banding and specific staining. Great choice!
Date published: 2017-01-09
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Strong band in WB in human transfected 293TStrong band in WB in human transfected 293T and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-01-19
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Thrombin 抗体 (F-1) is rated 4.6 out of 5 by 5.
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