Date published: 2025-9-7
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TGFβ2 Antibody (H-6) - Western Blotting - Image 155085
TGFβ2 (H-6): sc-374659. 人重组 TGFβ2 融合蛋白的 Western 印迹分析.
TGFβ2 Antibody (H-6) - Western Blotting - Image 155085
TGFβ2 (H-6): sc-374659. 人重组 TGFβ2 融合蛋白的 Western 印迹分析.
TGFβ2 (H-6): sc-374659. 人重组 TGFβ2 融合蛋白的 Western 印迹分析.

TGFβ2 抗体 (H-6): sc-374659

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寫評論提問

说明书
  • TGF beta 2 抗体 H-6 是小鼠单克隆 IgG3 κ，TGFβ2抗体, 在1篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的TGFβ2的C-terminus的氨基酸343-377之间
  • 推荐用于 mouse, rat， human 和 avian 来源的mature and precursor forms of TGFβ2 WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 关于如何获取TGFβ2 (H-6): sc-374659的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是TGFβ2 Antibody (H-6) 适用于 WB 应用。 的首选辅助检测试剂，该试剂现与TGFβ2 Antibody (H-6) 搭配使用（请参阅下面的订购信息）。

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研究信息

関連項目

额外TGF抗体，包括TGF α, TGF β 1, TGF β 2, TGF β 3和TGF β

TGFβ2抗体（H-6）是一种IgG3 κ小鼠单克隆TGFβ2抗体（也称为TGFB2抗体、TGFβ2抗体或Cetermin抗体），可通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）以及酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的TGFβ2蛋白。TGFβ2抗体（H-6）为非偶联形式的抗TGFβ2抗体。转化生长因子β（TGFβs）最初因其能够在TGFα存在下促进大鼠NRK成纤维细胞的非锚定生长而被发现。现在人们认识到，TGFβs介导胚胎发育过程中发生的许多细胞-细胞相互作用。在哺乳动物中已经鉴定出三种TGFβ。TGFβ1、TGFβ2和TGFβ3均以前体蛋白的形式合成，它们非常相似，每一种都被切割以产生一个由112个氨基酸组成的多肽，该多肽仍然与分子的潜伏部分相关联。具有生物活性的TGFβ需要单体的二聚化（通常为同源二聚体）并释放潜伏肽部分。总体而言，TGFβ3蛋白的成熟区域与TGFβ1和TGFβ2的成熟区域具有约80%的同一性。然而，它们的分子的NH2末端或前体区域仅具有27%的序列同一性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TGFβ2 抗体 (H-6) 参考文献:

  1. 转化生长因子-β。生长调节肽家族。  |  Miller, DA., et al. 1990. Ann N Y Acad Sci. 593: 208-17. PMID: 2375595
  2. 转化生长因子 beta3 的分子特征。  |  ten Dijke, P., et al. 1990. Ann N Y Acad Sci. 593: 26-42. PMID: 2375596
  3. 重组 TGF-beta 1 合成为一种双组分潜伏复合物，与原生血小板潜伏 TGF-beta 1 复合物具有某些结构特征。  |  Wakefield, LM., et al. 1989. Growth Factors. 1: 203-18. PMID: 2629880
  4. 内耳早期发育过程中TGFβ2诱导的衰老。  |  Gibaja, A., et al. 2019. Sci Rep. 9: 5912. PMID: 30976015
  5. 鉴定转化生长因子β型基因家族的另一个成员。  |  ten Dijke, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 4715-9. PMID: 3164476
  6. TGFβ2介导的上皮-间充质转化和NF-κB通路激活是奥希替尼耐药的原因之一。  |  Jiang, XM., et al. 2021. Acta Pharmacol Sin. 42: 451-459. PMID: 32678313
  7. 人类胶质母细胞瘤衍生 T 细胞抑制因子（转化生长因子-β 基因家族的新成员）的互补 DNA。  |  de Martin, R., et al. 1987. EMBO J. 6: 3673-7. PMID: 3322813
  8. Sigma-1 受体激活对 TGFβ2 诱导的人小梁网状细胞细胞外基质变化具有保护作用  |  Tran, MN., et al. 2023. Life (Basel). 13: PMID: 37511956
  9. 人类转化生长因子-β互补 DNA 序列及在正常细胞和转化细胞中的表达。  |  Derynck, R., et al. Nature. 316: 701-5. PMID: 3861940
  10. 某些人类肿瘤细胞产生的转化生长因子：与表皮生长因子受体相互作用的多肽。  |  Todaro, GJ., et al. 1980. Proc Natl Acad Sci U S A. 77: 5258-62. PMID: 6254071
  11. 来自病毒转化细胞条件培养基的肉瘤生长因子由α型和β型转化生长因子组成。  |  Anzano, MA., et al. 1983. Proc Natl Acad Sci U S A. 80: 6264-8. PMID: 6604914
  12. TGFB2和人类同源基因HLX1在染色体1q上的连锁定位。  |  Nishimura, DY., et al. 1993. Genomics. 15: 357-64. PMID: 8095486

订购信息

产品名称产品编号规格价格数量收藏夹

TGFβ2 抗体 (H-6)

sc-374659
200 µg/ml
RMB2377.00

TGFβ2 (H-6): m-IgG3 BP-HRP 套装

sc-550583
200 µg Ab; 40 µg BP
RMB2662.00

TGFβ2 (H-6) 中和勝肽

sc-374659 P
100 µg/0.5 ml
RMB511.00

Regarding storage for long period of time (> 1 year). Can we keep this Ab at -20 in 50% glycerol ?

Asked by: calimero
Thank you for your question. We do not recommend ever freezing our primary antibodies. The antibody is vialed in a buffer than contains gelatin as a stabilizer protein, and when frozen the gelatin can sequester IgG out of solution and negatively impact antibody performance. For long term storage, we recommend storing at 4 degrees C. If you are concerned about contamination, then we would recommend aliquoting the antibody for long-term storage at 4 degrees C.
Answered by: Technical Support
Date published: 2017-05-18

What application is the blocking peptide sc-374659 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_6, h_5CST
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Rated 4 out of 5 by from Good productThis product worked well for western blotting at 1:500 dilution
Date published: 2018-02-18
Rated 4 out of 5 by from good functionthis antibody have the same result with TGFb1 in mouse brian tissue protein at WB ,the dilution is 1:500,the reslut is success.
Date published: 2017-10-01
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 3 out of 5 by from No band in airway smooth muscleNo band in western using 16ug total protein from human airway smooth muscle cell lysates (1:100).
Date published: 2017-07-31
Rated 5 out of 5 by from TGF beta2 antibodyThe mouse TGF beta2 monoclonal antibody is so nice for WB.
Date published: 2017-05-23
Rated 5 out of 5 by from expected result for WBI got expected result for WB analysis of the target protein ,the result shows correct band ,I am satisfied
Date published: 2017-01-18
Rated 5 out of 5 by from Good WB antibody in human recombinant TGFb2Good WB antibody in human recombinant TGFb2 fusion protein. -SCBT QC
Date published: 2013-10-30
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TGFβ2 抗体 (H-6) is rated 4.3 out of 5 by 7.
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