Date published: 2025-9-7

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TGase2 抗体 (E-3): sc-48387

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说明书
  • TGase2 抗体 E-3 是小鼠单克隆 IgG2b κ,TGase2抗体, 在13篇文献中引用,规格为200 µg/ml
  • 免疫human物种的TGase2的氨基酸451-687
  • TGase2 抗体 (E-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 TGase2
  • 抗TGase2抗体(E-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TGase2 (E-3): sc-48387免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 TGase2 抗体 (E-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TGase2 抗体 (E-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

TGase2 抗体(E-3)是一种小鼠单克隆 IgG2b kappa 轻链抗体,可通过西部印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附试验(ELISA)检测人源 TGase2 蛋白。TGase2 (E-3) 抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。TGase2 又称转谷氨酰胺酶 2,通过催化蛋白质交联所必需的异肽键的形成,在细胞过程中发挥着至关重要的作用。这种酶活性在哺乳动物表皮细胞末期分化形成交联细胞包膜(CE)时尤为重要,有助于表皮的结构完整性和屏障功能。CE通过转谷氨酰胺酶形成二硫键和γ-谷氨酰-赖氨酸异二肽键,从而增强皮肤对环境压力的抵御能力。TGase2 参与血液凝固、细胞分化和细胞凋亡,在正常生理和病理情况下都具有重要意义。人组织转谷氨酰胺酶(TGase2)是一种 687 个氨基酸的蛋白质,特异性地表达于内皮细胞,因此 TGase2 是血管生物学和疾病研究的一个重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TGase2 抗体 (E-3) 参考文献:

  1. 转谷氨酰胺酶 1 的新功能:通过形成酯键将长链欧米伽羟基甘油酰胺附着到内卷蛋白上。  |  Nemes, Z., et al. 1999. Proc Natl Acad Sci U S A. 96: 8402-7. PMID: 10411887
  2. 一氧化氮对转谷氨酰胺酶的调节。  |  Bernassola, F., et al. 1999. Ann N Y Acad Sci. 887: 83-91. PMID: 10668466
  3. 转谷氨酰胺酶的抑制剂 GTP 可被组织型转谷氨酰胺酶(TGase 2)水解,但不能被表皮型转谷氨酰胺酶(TGase 3)水解。  |  Hitomi, K., et al. 2000. Biosci Biotechnol Biochem. 64: 657-9. PMID: 10803976
  4. 人类转谷氨酰胺酶 1 基因的结构和组织。  |  Kim, IG., et al. 1992. J Biol Chem. 267: 7710-7. PMID: 1348508
  5. 小鼠巨噬细胞和人类内皮细胞组织转谷氨酰胺酶 cDNA 克隆的分离与鉴定。  |  Gentile, V., et al. 1991. J Biol Chem. 266: 478-83. PMID: 1670766
  6. 从培养的角质细胞中分子克隆出人表皮转谷氨酰胺酶 cDNA。  |  Yamanishi, K., et al. 1991. Biochem Biophys Res Commun. 175: 906-13. PMID: 1673840
  7. 癌症中的转谷氨酰胺酶 2。  |  Huang, L., et al. 2015. Am J Cancer Res. 5: 2756-76. PMID: 26609482
  8. 人类疾病中的转谷氨酰胺酶 2。  |  Szondy, Z., et al. 2017. Biomedicine (Taipei). 7: 15. PMID: 28840829
  9. 转谷氨酰胺酶 2 通过 BAF250a 多聚酶介导转录物调控。  |  Kim, HJ., et al. 2021. Genes Genomics. 43: 333-342. PMID: 33555506
  10. 转谷氨酰胺酶 2 抑制剂治疗乳糜泻。  |  Ray, K. 2021. Nat Rev Gastroenterol Hepatol. 18: 594. PMID: 34267378
  11. 沉默转谷氨酰胺酶基因 TGase2 和 TGase3 对冈比亚按蚊的心率有依赖性影响  |  Ramakrishnan, A. and Hillyer, JF. 2022. Insects. 13: PMID: 35886758
  12. 转谷氨酰胺酶在新型细胞粘附中的双重功能  |  Ueki, S., et al. 1996. J Cell Sci. 109 (Pt 11): 2727-35. PMID: 8937990

订购信息

产品名称产品编号规格价格数量收藏夹

TGase2 抗体 (E-3)

sc-48387
200 µg/ml
$316.00

TGase2 (E-3): m-IgG Fc BP-HRP 套装

sc-528538
200 µg Ab; 10 µg BP
$354.00

TGase2 (E-3): m-IgGκ BP-HRP 套装

sc-520935
200 µg Ab, 40 µg BP
$354.00

TGase2 (E-3): m-IgG2b BP-HRP 套装

sc-550050
200 µg Ab; 10 µg BP
$354.00

TGase2 抗体 (E-3) AC

sc-48387 AC
500 µg/ml, 25% agarose
$416.00

TGase2 抗体 (E-3) HRP

sc-48387 HRP
200 µg/ml
$316.00

TGase2 抗体 (E-3) FITC

sc-48387 FITC
200 µg/ml
$330.00

TGase2 抗体 (E-3) PE

sc-48387 PE
200 µg/ml
$343.00

TGase2 抗体 (E-3) Alexa Fluor® 488

sc-48387 AF488
200 µg/ml
$357.00

TGase2 抗体 (E-3) Alexa Fluor® 546

sc-48387 AF546
200 µg/ml
$357.00

TGase2 抗体 (E-3) Alexa Fluor® 594

sc-48387 AF594
200 µg/ml
$357.00

TGase2 抗体 (E-3) Alexa Fluor® 647

sc-48387 AF647
200 µg/ml
$357.00

TGase2 抗体 (E-3) Alexa Fluor® 680

sc-48387 AF680
200 µg/ml
$357.00

TGase2 抗体 (E-3) Alexa Fluor® 790

sc-48387 AF790
200 µg/ml
$357.00

Im perfoming IF with sc-48387 anti-TG2 ab (1:50). Paraffin embedded tissue. 2º ab: Alexa555 gt-anti ms (1:200) Tried several antigen retrieval Blocking: NGS 5% Tween20 0.1%. I never get any signal, only precipitates. Need help

Asked by: Javi25
Thank you for your question. Please contact our Technical Service Department or reach out directly to your local distributor for assistance in troubleshooting this antibody. We are happy to help!
Answered by: Tech Service
Date published: 2019-10-03

I'm intending to use it for both IHC and ELISA testing. How many slides  and serum would 1 vial stain?!

Asked by: MMRD
Thank you for your question. We provide this mouse monoclonal, TGase2 (E-3): sc-48387, at 200 µg in 1 ml volume. The number of slides you could stain for IHC with this amount of antibody is going to depend on your conditions. We recommend a starting dilution of 1:50, but the primary antibody concentration should be titrated for optimal use on your samples, and could be anywhere within a range of 1:50-1:1,000. The volume you use for your samples will have to be determined experimentally.
Answered by: Technical Support
Date published: 2017-05-12

Can TGase2 (E-3): sc-48387 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, TGase2 (E-3): sc-48387 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from TGase2 antibodyTGase2 antibody used for checking expression level in TM cells used western analysis.
Date published: 2023-06-30
Rated 2 out of 5 by from Poor for western blotsLots of background but no bands with this antibody, even with 60 micrograms of protein from cell lines with robust TG2 expression.
Date published: 2018-02-21
Rated 5 out of 5 by from Also suitable for chickenI applied a sample product, this antibody got very good application in chicken lung tissue, the dilution ratio is 1: 200
Date published: 2017-12-18
Rated 5 out of 5 by from Good antibodyThis antibody shows a specific band using whole cell extract.
Date published: 2017-10-30
Rated 5 out of 5 by from Effective antibodyI got this antibody several months ago, it was effective and I have got satisfactory results.
Date published: 2017-04-05
Rated 5 out of 5 by from Effect is obvious.In our laboratory, 1:500 was stained with this antibody to make stained cells. Effect is obvious, very popular.
Date published: 2017-02-09
Rated 5 out of 5 by from This antibody is good for IHC experimentI use this antibody do IHC in lung cancer tissues, the result is very good.
Date published: 2017-01-18
Rated 5 out of 5 by from Strong band of interest observed in WB in HUVStrong band of interest observed in WB in HUV-EC-C and TF-1 whole cell lysates. -SCBT QC
Date published: 2015-05-14
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TGase2 抗体 (E-3) is rated 4.7 out of 5 by 11.
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