Date published: 2025-9-8

021-6093-6350

SCBT Portrait Logo
Seach Input

TFIIIB90-1 抗体 (BRF1G2A8): sc-81405

5.0(6)
寫評論提問

说明书
  • TFIIIB90-1 抗体 BRF1G2A8 是小鼠单克隆 IgG1TFIIIB90-1抗体, 在9篇文献中引用,规格为100 µg/ml
  • 针对与human TFIIIB90-1 C-terminus附近区域相对应的重组蛋白
  • 推荐用于 human 来源的TFIIIB90-1 WB 和 IP检测
  • 目前,我们还没有完成TFIIIB90-1 抗体 (BRF1G2A8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

関連項目

TFIIIB90-1抗体(BRF1G2A8)是一种IgG1小鼠单克隆TFIIIB90-1抗体,也可称为TFIIIB90-1抗体,它能够通过蛋白质印迹(WB)和免疫沉淀(IP)检测到人源的TFIIIB90-1蛋白。TFIIIB90-1抗体(BRF1G2A8)为非偶联形式的抗TFIIIB90-1抗体。RNA聚合酶III(pol III)负责合成tRNA、5s rRNA、7SL RNA和U6 snRNA,并且在许多转化的细胞系和体内的肿瘤中会过度表达,这是因为细胞在分裂之前必须复制其蛋白质成分。因此,为了维持快速生长,细胞必须产生大量的Pol III转录RNA,而这需要TFIIIB和TFIIIC2转录因子复合物的存在。TFIIIC2复合物由五个亚基组成,包括TFIIIC220、TFIIIC110、TFIIIC102、TFIIIC90和TFIIIC63,这些亚基在腺病毒转化的细胞以及体内的恶性细胞(如卵巢癌)中会过度表达。TFIIIC2能够募集RNA pol III和TFIIIB到启动子元件上,并可能是恶性细胞调控失常的关键组成部分。TFIIIB复合物包含TATA结合蛋白(TBP)、TFIIB相关因子1(TFIIIB90,BRF1)和TFIIIB'',这些成分在转化的细胞中的表达也会上调。在许多癌症中,肿瘤抑制因子视网膜母细胞瘤(RB)和p53会被灭活,这会影响它们结合并灭活TFIIIB功能的能力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TFIIIB90-1 抗体 (BRF1G2A8) 参考文献:

  1. TFIIIC 的 TFIIIC90 亚基与 RNA 聚合酶 III 机器的多个组件相互作用,并含有组蛋白特异性乙酰转移酶活性。  |  Hsieh, YJ., et al. 1999. Mol Cell Biol. 19: 7697-704. PMID: 10523658
  2. 含四肽重复转录因子 TFIIIC131 与其配体 TFIIIB70 之间的相互作用。复合体构象变化的证据。  |  Moir, RD., et al. 2000. J Biol Chem. 275: 26591-8. PMID: 10859316
  3. 替代剪接的 hBRF 变体在不同的 RNA 聚合酶 III 启动子上发挥作用。  |  McCulloch, V., et al. 2000. EMBO J. 19: 4134-43. PMID: 10921893
  4. 人类 TFIIIB 从含 TATA 和不含 TATA 的启动子引导 RNA 聚合酶 III 转录的活性不同。  |  Schramm, L., et al. 2000. Genes Dev. 14: 2650-63. PMID: 11040218
  5. RNA 聚合酶 III 转录因子 TFIIIC2 在卵巢肿瘤中过度表达。  |  Winter, AG., et al. 2000. Proc Natl Acad Sci U S A. 97: 12619-24. PMID: 11058163
  6. 视网膜母细胞瘤蛋白会破坏 RNA 聚合酶 III 转录所需的相互作用。  |  Sutcliffe, JE., et al. 2000. Mol Cell Biol. 20: 9192-202. PMID: 11094071
  7. RNA 聚合酶 III 转录:肿瘤抑制因子对其的控制以及转化因子对其的调控。  |  Brown, TR., et al. 2000. Gene Expr. 9: 15-28. PMID: 11097422
  8. 激活转化细胞中的小鼠基因。  |  Scott, MR., et al. 1983. Cell. 34: 557-67. PMID: 6311432
  9. 神经 BC1 RNA 的表达:在小鼠肿瘤中的诱导。  |  Chen, W., et al. 1997. Eur J Cancer. 33: 288-92. PMID: 9135502

订购信息

产品名称产品编号规格价格数量收藏夹

TFIIIB90-1 抗体 (BRF1G2A8)

sc-81405
100 µg/ml
RMB2504.00

I am using TFIIIB90-1 (BRF1G2A8): sc-81405 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_81405, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 114ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from very good resultThis antibody have been used published many papers,I use it for study Human liver tissue expression , it shows high expression in this sample by WB, I got the specific band by diluted the antibody 1:800.
Date published: 2017-06-25
Rated 5 out of 5 by from worked wellWorks extremely well in WB at a 1:500 dilution using HeLa cell lysate.
Date published: 2017-05-28
Rated 5 out of 5 by from Worked with WBGood antibody with clear signal and no background, the best part is that it's not too strong to saturate the band.
Date published: 2017-05-07
Rated 5 out of 5 by from worked greatworked great on human brains both by western blot. The dilution I used was 1:1000.
Date published: 2017-04-30
Rated 5 out of 5 by from Well doneSpecial bands is detected when the dilution rate is 1:500, it works well in WB.
Date published: 2016-11-16
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_5
  • loc_zh_CN, sid_81405, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
TFIIIB90-1 抗体 (BRF1G2A8) is rated 5.0 out of 5 by 6.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_5
  • loc_zh_CN, sid_81405, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 122ms
  • REVIEWS, PRODUCT