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TCR α Antibody (H-1) - Western Blotting - Image 379603 — TCR α 抗体 (H-1): sc-515719. SUP-T1 (A) 和 MOLT-4 (B) 全细胞裂解液中 TCR α 表达的 Western 印迹分析.

TCR α 抗体 (H-1): sc-515719. SUP-T1 (A) 和 MOLT-4 (B) 全细胞裂解液中 TCR α 表达的 Western 印迹分析.

TCR α 抗体 (H-1): sc-515719

Name: TCR &alpha;抗体 (H-1)
Brand: SCBIO
SKU: sc-515719
Price: 2377.0 CNY
Availability: InStock

4.5(2)

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说明书

TCR alpha 抗体 H-1 是小鼠单克隆 IgG_2a κ，TCR α抗体, 在6篇文献中引用，规格为200 µg/ml
针对human起源的TCR α恒定区域内的氨基酸1-142图谱
TCR alpha 抗体 (H-1) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 TCR α C region
抗TCR alpha抗体（H-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取TCR α (H-1): sc-515719的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP, m-IgG_2a BP-HRP 和 m-IgGκ BP-HRP 是 TCR α 抗体 (H-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TCR α 抗体 (H-1) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(6)

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TCR α 抗体 (H-1) 参考文献:

在 TCR 组装过程中，TCR-α 对 pT alpha 的竞争性置换可防止前 TCR 和 alpha beta TCR 在表面上共表达。 | Trop, S., et al. 2000. J Immunol. 165: 5566-72. PMID: 11067911
发育中胸腺细胞中 T 细胞受体基因重排的可及性控制。TCR α/δ位点。 | Krangel, MS., et al. 2000. Immunol Res. 22: 127-35. PMID: 11339350
克隆 TCR 重组系统揭示了 T 细胞受体（TCR）-α 链在形成免疫前 TCR 复合物中的主要作用。 | Yokosuka, T., et al. 2002. J Exp Med. 195: 991-1001. PMID: 11956290
自身和外来超抗原与小鼠 TCR alpha beta 的特异性 V beta 元素相互作用的结果。 | Kappler, JW., et al. 1989. Cold Spring Harb Symp Quant Biol. 54 Pt 1: 401-7. PMID: 2534796
粘膜相关不变 T 细胞上的 TCR-&α 双重表达是 TCR 解释的潜在干扰因素。 | Suliman, S., et al. 2022. J Immunol. 208: 1389-1395. PMID: 35246495
Tnpo3 控制着 iNKT 细胞中编码规范 TCR α 链的前 mRNA 的剪接。 | Iwanami, N., et al. 2023. Nat Commun. 14: 3645. PMID: 37339974
CD11a 和 CD45R 异构体的表达定义了体内不同的 CD8+ TCR alpha beta 和 TCR gamma delta CTL 亚群。 | Hedlund, G., et al. 1995. Immunol Rev. 146: 82-94. PMID: 7493762
未成熟 CD4+ CD8+ 胸腺细胞中 T 细胞受体（TCR）信号诱导的早期分子事件：TCR-α 蛋白的合成增加是对 TCR 信号的早期反应，可补偿 TCR-α 的不稳定性，改善 TCR 组装，并与其他正向选择指标并行。 | Kearse, KP., et al. 1995. J Exp Med. 181: 193-202. PMID: 7528767
T 细胞抗原受体 (TCR) 复合物的化学计量：每个 TCR/CD3 复合物包含一个 TCR alpha、一个 TCR beta 和两个 CD3 epsilon 链。 | Punt, JA., et al. 1994. J Exp Med. 180: 587-93. PMID: 8046335
TCR/CD3复合物的组装：CD3 epsilon/delta和CD3 epsilon/gamma二聚体与TCR alpha和TCR beta链结合不明确。双TCR异二聚体模型的证据。 | San José, E., et al. 1998. Eur J Immunol. 28: 12-21. PMID: 9485181

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
TCR α 抗体 (H-1)	sc-515719	200 µg/ml	RMB2377.00
TCR α (H-1): m-IgG Fc BP-HRP 套装	sc-531481	200 µg Ab; 10 µg BP	RMB2662.00
TCR α (H-1): m-IgGκ BP-HRP 套装	sc-525178	200 µg Ab, 40 µg BP	RMB2662.00
TCR α (H-1): m-IgG_2a BP-HRP 套装	sc-547800	200 µg Ab; 10 µg BP	RMB2662.00
TCR α 抗体 (H-1) AC	sc-515719 AC	500 µg/ml, 25% agarose	RMB3129.00
TCR α 抗体 (H-1) HRP	sc-515719 HRP	200 µg/ml	RMB2377.00
TCR α 抗体 (H-1) FITC	sc-515719 FITC	200 µg/ml	RMB2482.00
TCR α 抗体 (H-1) PE	sc-515719 PE	200 µg/ml	RMB2580.00
TCR α 抗体 (H-1) Alexa Fluor^® 488	sc-515719 AF488	200 µg/ml	RMB2685.00
TCR α 抗体 (H-1) Alexa Fluor^® 546	sc-515719 AF546	200 µg/ml	RMB2685.00
TCR α 抗体 (H-1) Alexa Fluor^® 594	sc-515719 AF594	200 µg/ml	RMB2685.00
TCR α 抗体 (H-1) Alexa Fluor^® 647	sc-515719 AF647	200 µg/ml	RMB2685.00
TCR α 抗体 (H-1) Alexa Fluor^® 680	sc-515719 AF680	200 µg/ml	RMB2685.00
TCR α 抗体 (H-1) Alexa Fluor^® 790	sc-515719 AF790	200 µg/ml	RMB2685.00

Does this antibody bind to the intracellular or extracellular domain of TCR α?

Asked by: KzHs

Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. お問い合わせありがとうございます。当社の技術サービス部門にお問い合わせください。当社のアジア技術サービスチームは、(+86)021-60936350または[email protected]、または当社のウェブサイトのライブチャットでご利用いただけます。

Answered by: Yanru Tao

Date published: 2025-01-06

I am using conjugated TCR alpha for flow staining . I have repeated several times with dilution of 1:100 and 1:20 and have had negative results . seems to me this AB has not been validated. Could you suggest any trouble shooting steps.

Asked by: Tara M

Thank for your question. Please contact our Technical Support at (800)-457-3801 or email [email protected]

Answered by: BlakeJ

Date published: 2022-09-07

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TCR α (H-1): sc-515719?

Asked by: cjMara

We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Technical Support

Date published: 2017-02-27

Rated 5 out of 5 by DrBrain from Good for western blotI've got anti-TCR (H-1) a free samples and it worked really well and considering placing an order for full size product.

Date published: 2017-09-23

Rated 4 out of 5 by JImmun from Worked well for Positive Ligation AssayWorked well when paired with TCR beta from different species for PLA.

Date published: 2017-09-01

TCR α 抗体 (H-1) is rated 4.5 out of 5 by 2.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

TCR α 抗体 (H-1): sc-515719

TCR α 抗体 (H-1) 参考文献:

TCR α 抗体 (H-1) 规格

替代产品名称: T Cell Receptor

产品克隆名称: H-1

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG_2a kappa light chain

氨基酸: 1-142

抗原表位靶标: TCR α

抗原表位种属: human

产品反应性/检测: TCR α C region

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 6篇各种科学出版物的引文

TCR α 抗体 (H-1)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 TCR α 抗體是 TCR α 抗體 (H28-710): sc-101410.

TCR α 抗体 (H-1)

TCR α (H-1): m-IgG Fc BP-HRP 套装

TCR α (H-1): m-IgGκ BP-HRP 套装

TCR α (H-1): m-IgG_2a BP-HRP 套装

TCR α 抗体 (H-1) AC

TCR α 抗体 (H-1) HRP

TCR α 抗体 (H-1) FITC

TCR α 抗体 (H-1) PE

TCR α 抗体 (H-1) Alexa Fluor^® 488

TCR α 抗体 (H-1) Alexa Fluor^® 546

TCR α 抗体 (H-1) Alexa Fluor^® 594

TCR α 抗体 (H-1) Alexa Fluor^® 647

TCR α 抗体 (H-1) Alexa Fluor^® 680

TCR α 抗体 (H-1) Alexa Fluor^® 790

Does this antibody bind to the intracellular or extracellular domain of TCR α?

I am using conjugated TCR alpha for flow staining . I have repeated several times with dilution of 1:100 and 1:20 and have had negative results . seems to me this AB has not been validated. Could you suggest any trouble shooting steps.

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TCR α (H-1): sc-515719?

TCR α 抗体 (H-1): sc-515719

TCR α 抗体 (H-1) 参考文献:

TCR α 抗体 (H-1) 规格

替代产品名称: T Cell Receptor

产品克隆名称: H-1

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG2a kappa light chain

氨基酸: 1-142

抗原表位靶标: TCR α

抗原表位种属: human

产品反应性/检测: TCR α C region

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 6篇各种科学出版物的引文

TCR α 抗体 (H-1)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 TCR α 抗體是 TCR α 抗體 (H28-710): sc-101410.

TCR α 抗体 (H-1)

TCR α (H-1): m-IgG Fc BP-HRP 套装

TCR α (H-1): m-IgGκ BP-HRP 套装

TCR α (H-1): m-IgG2a BP-HRP 套装

TCR α 抗体 (H-1) AC

TCR α 抗体 (H-1) HRP

TCR α 抗体 (H-1) FITC

TCR α 抗体 (H-1) PE

TCR α 抗体 (H-1) Alexa Fluor® 488

TCR α 抗体 (H-1) Alexa Fluor® 546

TCR α 抗体 (H-1) Alexa Fluor® 594

TCR α 抗体 (H-1) Alexa Fluor® 647

TCR α 抗体 (H-1) Alexa Fluor® 680

TCR α 抗体 (H-1) Alexa Fluor® 790

Does this antibody bind to the intracellular or extracellular domain of TCR &alpha;?

I am using conjugated TCR alpha for flow staining . I have repeated several times with dilution of 1:100 and 1:20 and have had negative results . seems to me this AB has not been validated. Could you suggest any trouble shooting steps.

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TCR &alpha; (H-1): sc-515719?

抗体同型: IgG_2a kappa light chain

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

TCR α 抗体 (H-1)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

TCR α (H-1): m-IgG_2a BP-HRP 套装

TCR α 抗体 (H-1) Alexa Fluor^® 488

TCR α 抗体 (H-1) Alexa Fluor^® 546

TCR α 抗体 (H-1) Alexa Fluor^® 594

TCR α 抗体 (H-1) Alexa Fluor^® 647

TCR α 抗体 (H-1) Alexa Fluor^® 680

TCR α 抗体 (H-1) Alexa Fluor^® 790

Does this antibody bind to the intracellular or extracellular domain of TCR α?

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with TCR α (H-1): sc-515719?