Date published: 2025-9-6

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TCP-1 α 抗体 (91A): sc-53454

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说明书
  • TCP-1 alpha 抗体 91A 是大鼠单克隆 IgG2aTCP-1 α抗体, 在12篇文献中引用,规格为200 µg/ml
  • 针对murine来源的TCP的C-terminal一半
  • TCP-1 alpha 抗体 (91A) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 TCP-1α
  • 抗TCP-1 alpha抗体(91A)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TCP-1 α (91A): sc-53454免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成TCP-1 α 抗体 (91A)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

TCP-1 α 抗体(91A)是一种大鼠单克隆 IgG2a 抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的 TCP-1 α 蛋白。TCP-1 α (91A) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。TCP-1,也称为t复合多肽1,是TCP-1(CCT)复合物中一个至关重要的亚基,对于细胞质蛋白(尤其是肌动蛋白和微管蛋白)的正常折叠至关重要。CCT复合物位于真核细胞质中,由八个不同的亚基组成,每个亚基由不同的基因编码,对于维持细胞功能和完整性至关重要。TCP-1在协助蛋白质折叠方面起着尤为重要的作用,因为蛋白质折叠错误会导致细胞功能紊乱,引发各种疾病。TCP-1最初在小鼠体内被发现,小鼠的突变与无尾和胚胎致死性表型有关,而在其他物种中也发现了同源序列,这凸显了TCP-1在进化中的重要性。酵母菌版本的TCP-1充当肌动蛋白和微管蛋白的分子伴侣,这凸显了其在不同生物体中功能的保守性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TCP-1 α 抗体 (91A) 参考文献:

  1. 细胞膜伴侣素 CCT 亚基在从导致未折叠蛋白质积累的化学应激中恢复的过程中上调。  |  Yokota, SI., et al. 2000. Eur J Biochem. 267: 1658-64. PMID: 10712596
  2. 曼氏血吸虫伴侣素 t-复合物多肽 1 基因的克隆及其在热休克和氧化应激下的表达水平研究。  |  Campos, EG. and Hamdan, FF. 2000. Parasitol Res. 86: 253-8. PMID: 10726997
  3. 真核细胞胞质合子蛋白的各个亚基介导与位于β-肌动蛋白亚域上的结合位点的相互作用。  |  Hynes, GM. and Willison, KR. 2000. J Biol Chem. 275: 18985-94. PMID: 10748209
  4. 确定人类小管蛋白中的真核细胞胞质伴侣素结合位点。  |  Ritco-Vonsovici, M. and Willison, KR. 2000. J Mol Biol. 304: 81-98. PMID: 11071812
  5. 拟南芥 CRY1 的信号机制涉及与 COP1 的直接相互作用。  |  Yang, HQ., et al. 2001. Plant Cell. 13: 2573-87. PMID: 11752373
  6. 细胞凋亡过程中抑制 CDP-choline 通路的机制  |  Morton, CC., et al. 2013. J Lipid Res. 54: 3373-84. PMID: 24136823
  7. 核定位的 CTP:磷脂酰胆碱胞苷酸转移酶α;调节脂滴生物形成所需的磷脂酰胆碱合成。  |  Aitchison, AJ., et al. 2015. Mol Biol Cell. 26: 2927-38. PMID: 26108622
  8. 巨噬细胞中磷脂酰胆碱的加速周转促进了肥胖症的脂肪组织炎症。  |  Petkevicius, K., et al. 2019. Elife. 8: PMID: 31418690
  9. 与脂质相关的 PML 结构组装了含有 CCTα 和 Lipin1 的核脂滴。  |  Lee, J., et al. 2020. Life Sci Alliance. 3: PMID: 32461215
  10. 膀胱癌患者膀胱切除术前新辅助全身化疗的生物标志物组合。  |  Türker, P., et al. 2021. Transl Res. 235: 77-84. PMID: 33766713
  11. 黄瓜 T 复合蛋白。在子叶和下胚轴的 22-S 细胞质复合体中的分子克隆, 细菌表达和表征。  |  Ahnert, V., et al. 1996. Eur J Biochem. 235: 114-9. PMID: 8631317
  12. 盘基竹荪与 Tcp-1 的同源物对生长和发育至关重要。  |  Iijima, M., et al. 1998. Gene. 213: 101-6. PMID: 9630545

订购信息

产品名称产品编号规格价格数量收藏夹

TCP-1 α 抗体 (91A)

sc-53454
200 µg/ml
RMB2377.00

TCP-1 α 抗体 (91A) AC

sc-53454 AC
500 µg/ml, 25% agarose
RMB3129.00

TCP-1 α 抗体 (91A) HRP

sc-53454 HRP
200 µg/ml
RMB2377.00

TCP-1 α 抗体 (91A) FITC

sc-53454 FITC
200 µg/ml
RMB2482.00

TCP-1 α 抗体 (91A) PE

sc-53454 PE
200 µg/ml
RMB2580.00

TCP-1 α 抗体 (91A) Alexa Fluor® 488

sc-53454 AF488
200 µg/ml
RMB2685.00

TCP-1 α 抗体 (91A) Alexa Fluor® 546

sc-53454 AF546
200 µg/ml
RMB2685.00

TCP-1 α 抗体 (91A) Alexa Fluor® 594

sc-53454 AF594
200 µg/ml
RMB2685.00

TCP-1 α 抗体 (91A) Alexa Fluor® 647

sc-53454 AF647
200 µg/ml
RMB2685.00

TCP-1 α 抗体 (91A) Alexa Fluor® 680

sc-53454 AF680
200 µg/ml
RMB2685.00

TCP-1 α 抗体 (91A) Alexa Fluor® 790

sc-53454 AF790
200 µg/ml
RMB2685.00

Are there any issues with using sc-53454: TCP-1 α (91A) mouse monoclonal primary antibody on mouse samples?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53454 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Great for WB, not working for IPThis antibody works great in Western Blot, I used it at 1:500 and produced a clean and strong signal. However I needed it for inmunoprecipitation and it didn´t work.
Date published: 2018-03-21
Rated 5 out of 5 by from a fantastic antibody for WBThis antibody recognizes a clear and strong signal at the predicted M.W. (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-20
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 5 out of 5 by from Good Western Blots For PublicationIt was tested by Surgery and Oncology Department in Vanderbilt University recently. This Antibody produces high quality western blots results in Barrett's esophagus (BE) cells and in esophageal adenocarcinoma (EAC) cells, which is good for publication.
Date published: 2017-04-30
Rated 5 out of 5 by from Reliable Antibody, Great for IHCThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-08
Rated 5 out of 5 by from Very good antibody for western blotI used this antibody on murine cell line. It gives very nice results by western blot.
Date published: 2017-02-07
Rated 5 out of 5 by from Published data for WB and IF in rabbit heartPublished data for WB and IF in rabbit heart tissue. -SCBT Publication Review
Date published: 2015-05-24
Rated 5 out of 5 by from Published data for WB in human TRiC control samplePublished data for WB in human TRiC control sample. -SCBT Publication Review
Date published: 2015-05-15
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TCP-1 α 抗体 (91A) is rated 4.9 out of 5 by 11.
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