Date published: 2025-9-7

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TBR-1 抗体 (G-5): sc-376258

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说明书
  • TBR-1 抗体 G-5 是小鼠单克隆 IgG2b κ,TBR-1抗体, 在5篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-200位于mouse物种的TBR-1的N-terminus
  • TBR-1 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 TBR-1
  • 抗TBR-1抗体(G-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376258 X, 200 µg/0.1 ml)
  • 关于如何获取TBR-1 (G-5): sc-376258免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是TBR-1 Antibody (G-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与TBR-1 Antibody (G-5) 打包提供(请参阅下面的订购信息)。

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関連項目

TBR-1抗体(G-5)是一种IgG2b κ小鼠单克隆TBR-1抗体(也称为TBR-1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的TBR-1蛋白。TBR-1抗体(G-5)既有非偶联型抗TBR-1抗体形式,也有多种偶联型抗TBR-1抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。一种新型的小鼠和人类基因TBR-1编码一种假定的与Brachyury(T)基因相关的转录因子,该基因仅在分裂后细胞中表达。T-brain-1(TBR-1)mRNA主要限于大脑皮层,在胚胎发育期间,它定义了产生古皮层、边缘皮层和新皮层的不同区域。TBR-1、Pax-6和Emx-1在小鼠和鸡的大脑皮层中表达。皮层下边界位于TBR-1和Dlx-2表达域之间。在发育过程中,与这些小鼠基因同源的鸡基因以拓扑结构相似的模式表达,这表明小鼠和鸡可能具有相似的组织发生过程和场同源性。CASK/LIN-2是一种膜相关的鸟苷酸激酶,对EGFR定位和信号传导是必需的。在成年大鼠脑中,CASK集中在神经元突触处并与细胞表面蛋白结合。CASK可以与参与前脑发育的TBR-1相互作用。CASK进入细胞核并与TBR-1形成复合物,结合特定的DNA序列(T元素)。因此,CASK作为TBR-1的共激活因子,诱导含有T元素的基因(包括reelin)转录。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TBR-1 抗体 (G-5) 参考文献:

  1. 膜缔合作用鸟苷酸激酶 CASK/LIN-2 的核转位和转录调控。  |  Hsueh, YP., et al. 2000. Nature. 404: 298-302. PMID: 10749215
  2. 小鼠 T-brain-1 的海星同源基因在 Asterina pectinifera 胚胎的弓形体中表达:两个 T-box 基因可能参与了海星的胃形成。  |  Shoguchi, E., et al. 2000. Dev Growth Differ. 42: 61-8. PMID: 10831044
  3. 通过 Dlx-2, Emx-1, Nkx-2.1, Pax-6 和 Tbr-1 基因的表达追踪胚胎雏鸡和小鼠端脑中的髓质和髓质下衍生物。  |  Puelles, L., et al. 2000. J Comp Neurol. 424: 409-38. PMID: 10906711
  4. 斑马鱼前脑发育过程中 T-brain-1 和 eomesodermin 的重叠表达。  |  Mione, M., et al. 2001. Mech Dev. 100: 93-7. PMID: 11118891
  5. 成年啮齿动物大脑中 T-box 转录因子 Tbr-1 的细胞质分布。  |  Hong, CJ. and Hsueh, YP. 2007. J Chem Neuroanat. 33: 124-30. PMID: 17329080
  6. Tbr1单倍体缺陷会损害杏仁轴突突生,并导致认知异常。  |  Huang, TN., et al. 2014. Nat Neurosci. 17: 240-7. PMID: 24441682
  7. TBR1 在发育中的新皮层中调控自闭症风险基因。  |  Notwell, JH., et al. 2016. Genome Res. 26: 1013-22. PMID: 27325115
  8. Tbr1表达错误会改变大脑皮层神经元的发育  |  Crespo, I., et al. 2022. Mol Neurobiol. 59: 5750-5765. PMID: 35781633
  9. 患者特异性 Tbr1 基因突变对皮层发育的共同和不同功能影响  |  Co, M., et al. 2022. J Neurosci. 42: 7166-7181. PMID: 35944998
  10. 利用聚合酶链式反应比较 ITS-1 和 TBR-1/2 引物检测实验鼠体内伊万斯锥虫本地分离株的灵敏度。  |  Suprihati, E., et al. 2022. Vet World. 15: 1772-1778. PMID: 36185509
  11. TBR1 相互作用组的特征:与神经发育障碍相关的变体破坏了新的蛋白质相互作用。  |  Sollis, E., et al. 2023. Hum Mol Genet. 32: 1497-1510. PMID: 36579832
  12. T-脑-1:Brachyury 的同源物,其表达定义了大脑皮层内不同的分子区域。  |  Bulfone, A., et al. 1995. Neuron. 15: 63-78. PMID: 7619531

订购信息

产品名称产品编号规格价格数量收藏夹

TBR-1 抗体 (G-5)

sc-376258
200 µg/ml
RMB2377.00

TBR-1 (G-5): m-IgGκ BP-HRP 套装

sc-522898
200 µg Ab, 40 µg BP
RMB2662.00

TBR-1 (G-5): m-IgG2b BP-HRP 套装

sc-549080
200 µg Ab; 10 µg BP
RMB2662.00

TBR-1 抗体 (G-5) X

sc-376258 X
200 µg/0.1 ml
RMB2377.00

TBR-1 抗体 (G-5) AC

sc-376258 AC
500 µg/ml, 25% agarose
RMB3129.00

TBR-1 抗体 (G-5) HRP

sc-376258 HRP
200 µg/ml
RMB2377.00

TBR-1 抗体 (G-5) FITC

sc-376258 FITC
200 µg/ml
RMB2482.00

TBR-1 抗体 (G-5) PE

sc-376258 PE
200 µg/ml
RMB2580.00

TBR-1 抗体 (G-5) Alexa Fluor® 488

sc-376258 AF488
200 µg/ml
RMB2685.00

TBR-1 抗体 (G-5) Alexa Fluor® 546

sc-376258 AF546
200 µg/ml
RMB2685.00

TBR-1 抗体 (G-5) Alexa Fluor® 594

sc-376258 AF594
200 µg/ml
RMB2685.00

TBR-1 抗体 (G-5) Alexa Fluor® 647

sc-376258 AF647
200 µg/ml
RMB2685.00

TBR-1 抗体 (G-5) Alexa Fluor® 680

sc-376258 AF680
200 µg/ml
RMB2685.00

TBR-1 抗体 (G-5) Alexa Fluor® 790

sc-376258 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-376258: TBR-1 (G-5) mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 4 out of 5 by from Good!It works nicely in IF of mouse primary neurons! Bright signal is visible.
Date published: 2025-01-27
Rated 4 out of 5 by from High specificity and good IHC resultsI purchursed this antibody and used for an alternitive of the discontinuous one fro this same company, and from the images I got, I'm pretty satisfied with this product. Easy to use.
Date published: 2019-04-23
Rated 5 out of 5 by from Clear bandsI used the sample for my western blot and the bands were very clear and nice. thanks.
Date published: 2018-02-23
Rated 1 out of 5 by from Poor results for IF stainingI requested a sample of this antibody for an IF stain. Unfortunately, the staining did not work.
Date published: 2018-01-29
Rated 4 out of 5 by from works good in immunoblotting but not in IFThe antibody gives a clean and specific band in immunoblotting of human neural culture samples. However it didn't work in immunostainings of samples that were run in parallel.
Date published: 2018-01-24
Rated 1 out of 5 by from not workingBoth my colleague and me tried this antibody with and without antigen retrieval, unfortunately it didn't work.
Date published: 2018-01-15
Rated 5 out of 5 by from Great productthe product is very good ,i'm very appreciate to it
Date published: 2017-01-04
Rated 5 out of 5 by from It's helpfulIt's helpful to our research.The sections were then incubated with this antibody 4 hours at 24°C.
Date published: 2016-11-30
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TBR-1 抗体 (G-5) is rated 3.8 out of 5 by 9.
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