Date published: 2025-9-7
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TAL1 Antibody (C-4) - Western Blotting - Image 354668
TAL1 抗体 (C-4): sc-393287. Western 印迹分析 Jurkat 细胞核提取物中 TAL1 的表达.
TAL1 Antibody (C-4) - Western Blotting - Image 354668
TAL1 抗体 (C-4): sc-393287. Western 印迹分析 Jurkat 细胞核提取物中 TAL1 的表达.
TAL1 Antibody (C-4) - Western Blotting - Image 294516
TAL1 抗体 (C-4): sc-393287. 非转染: sc-117752 (A) 和人 TAL1 转染: sc-172270 (B) 293T 全细胞裂解液以及 K-562 (C), Jurkat (D) 和 CCRF-CEM (E) 核提取物中 TAL1 表达的 Western 印迹分析.
TAL1 抗体 (C-4): sc-393287. Western 印迹分析 Jurkat 细胞核提取物中 TAL1 的表达.

TAL1 抗体 (C-4): sc-393287

4.2(5)
寫評論提問

说明书
  • TAL1 抗体 C-4 是小鼠单克隆 IgG1 κ，TAL1抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列272-331位于human物种的TAL1的C-terminus
  • TAL1 抗体 (C-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 TAL1 pp42, pp39 and pp22 isoforms; 也和以下物种反应，包括： canine
  • 抗TAL1抗体（C-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-393287 X, 200 µg/0.1 ml)
  • 关于如何获取TAL1 (C-4): sc-393287的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是TAL1 Antibody (C-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与TAL1 Antibody (C-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外TAL抗体，包括TAL2

TAL1抗体（C-4）是一种IgG1 κ小鼠单克隆TAL1抗体（也被称为TAL BHLH转录因子1抗体、红细胞分化因子抗体、BHLHa17抗体、T细胞急性淋巴细胞白血病蛋白1抗体、A类基础螺旋-环-螺旋蛋白17抗体、T细胞白血病/淋巴瘤蛋白5抗体、干细胞蛋白抗体、TCL5抗体或BHLHA17抗体），它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的TAL1蛋白。TAL1抗体（C-4）既可用作非偶联的抗TAL1抗体形式，也可用作多种偶联形式的抗TAL1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在人类T细胞急性淋巴细胞白血病中，TAL1的激活高达60%的病例，使其成为该疾病中最常见的功能获得性突变。TAL1（也被称为SCL）是一种丝氨酸磷酸蛋白和基本的螺旋-环-螺旋转录因子，已知可调节胚胎造血过程。这种转录因子与E2A和HEB/HTF4结合形成异二聚体，并结合到一个被称为E-box的核苷酸序列基序上。此外，TAL1与酪蛋白激酶IIalpha的催化亚基（一种已知的丝氨酸/苏氨酸蛋白激酶，可调节其他bHLH转录因子的活性）的转基因共表达会显著加速白血病的发生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TAL1 抗体 (C-4) 参考文献:

  1. mSin3A通过与TAL1（或SCL）转录因子缔合作用，调控小鼠红白血病细胞分化。  |  Huang, S. and Brandt, SJ. 2000. Mol Cell Biol. 20: 2248-59. PMID: 10688671
  2. 与 E47/E12 螺旋环-螺旋蛋白缔合的 tal-1 肿瘤蛋白的增强子结合活性。  |  Hsu, HL., et al. 1991. Mol Cell Biol. 11: 3037-42. PMID: 2038315
  3. T细胞急性淋巴细胞白血病的表观遗传学图景。  |  Van der Meulen, J., et al. 2014. Int J Biochem Cell Biol. 53: 547-57. PMID: 24786297
  4. microRNAs调节T细胞急性淋巴细胞白血病中的TAL1表达。  |  Correia, NC., et al. 2016. Oncotarget. 7: 8268-81. PMID: 26882564
  5. 造血和细胞重编程中的SCL/TAL1。  |  Hoang, T., et al. 2016. Curr Top Dev Biol. 118: 163-204. PMID: 27137657
  6. TAL1作为T细胞急性淋巴细胞白血病中的主要致癌转录因子。  |  Sanda, T. and Leong, WZ. 2017. Exp Hematol. 53: 7-15. PMID: 28652130
  7. TAL1在T细胞急性淋巴细胞白血病中驱动的致癌转录程序。  |  Tan, TK., et al. 2019. Int J Hematol. 109: 5-17. PMID: 30145780
  8. TAL1转录因子的异构体在造血和细胞生长中具有不同的作用。  |  Sharma, A., et al. 2023. PLoS Biol. 21: e3002175. PMID: 37379322
  9. KLF4抑制T细胞急性淋巴细胞白血病中由超级增强子驱动的TAL1表达。  |  Noura, M., et al. 2024. Oncogene. 43: 447-456. PMID: 38102337
  10. 大多数 T 细胞急性淋巴细胞白血病患者的 TAL1 基因会被激活吗？儿科肿瘤学小组的一项研究。  |  Bash, RO., et al. 1995. Blood. 86: 666-76. PMID: 7605997
  11. 利用新型单克隆抗体 2TL 242 对白血病和淋巴瘤中的 TAL-1 蛋白表达进行免疫组化研究。  |  Chetty, R., et al. 1996. J Pathol. 178: 311-5. PMID: 8778337
  12. Tal-1 通过酪蛋白激酶 IIalpha 诱导 T 细胞急性淋巴细胞白血病加速发展。  |  Kelliher, MA., et al. 1996. EMBO J. 15: 5160-6. PMID: 8895560

订购信息

产品名称产品编号规格价格数量收藏夹

TAL1 抗体 (C-4)

sc-393287
200 µg/ml
RMB2377.00

TAL1 (C-4): m-IgG Fc BP-HRP 套装

sc-530418
200 µg Ab; 10 µg BP
RMB2662.00

TAL1 (C-4): m-IgGκ BP-HRP 套装

sc-523887
200 µg Ab, 40 µg BP
RMB2662.00

TAL1 (C-4): m-IgG1 BP-HRP 套装

sc-544043
200 µg Ab; 20 µg BP
RMB2662.00

TAL1 抗体 (C-4) X

sc-393287 X
200 µg/0.1 ml
RMB2377.00

TAL1 抗体 (C-4) AC

sc-393287 AC
500 µg/ml, 25% agarose
RMB3129.00

TAL1 抗体 (C-4) HRP

sc-393287 HRP
200 µg/ml
RMB2377.00

TAL1 抗体 (C-4) FITC

sc-393287 FITC
200 µg/ml
RMB2482.00

TAL1 抗体 (C-4) PE

sc-393287 PE
200 µg/ml
RMB2580.00

TAL1 抗体 (C-4) Alexa Fluor® 488

sc-393287 AF488
200 µg/ml
RMB2685.00

TAL1 抗体 (C-4) Alexa Fluor® 546

sc-393287 AF546
200 µg/ml
RMB2685.00

TAL1 抗体 (C-4) Alexa Fluor® 594

sc-393287 AF594
200 µg/ml
RMB2685.00

TAL1 抗体 (C-4) Alexa Fluor® 647

sc-393287 AF647
200 µg/ml
RMB2685.00

TAL1 抗体 (C-4) Alexa Fluor® 680

sc-393287 AF680
200 µg/ml
RMB2685.00

TAL1 抗体 (C-4) Alexa Fluor® 790

sc-393287 AF790
200 µg/ml
RMB2685.00

该抗体可以用于冰冻切片免疫荧光染色吗

Asked by: 赵 啾啾
Thank you for your question. Yes, this antibody is recommended for immunofluorescence staining of frozen sections for mouse, rat and human. thank you. 感谢您的提问。是的，该抗体推荐用于小鼠、大鼠和人类冷冻切片的免疫荧光染色。谢谢。
Answered by: Jiajun Z
Date published: 2025-01-16

can it be used for cut & run?

Asked by: jun113
Thank you for your question. This antibody has not been tested in CUT&RUN sequencing applications.
Answered by: Tech Support Europe
Date published: 2020-12-16

What's the work concentration for WB?

Asked by: Cong
Thank you for your question. We recommend using this at the starting dilution of 1:200 (range 1:100-1:1,000) for Western blotting.
Answered by: Technical Support
Date published: 2019-04-20

Are there any issues with using sc-393287: TAL1 (C-4) mouse monoclonal primary antibody on mouse samples?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-393287 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from Unfortunately did not work on mouse brain tissueWe tried Immunofluorescence (1:200) on cryosections from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0). Unfortunately, we saw no signal.
Date published: 2017-11-09
Rated 5 out of 5 by from very niceTAL1 antibody with a precise position effect and very powerful signal in Jurkat nuclear extract. The effect is very good.
Date published: 2017-10-22
Rated 5 out of 5 by from Good AntibodyIn the experiment, TAL1 antibody has high expression and extra strong signal in Jurkat nuclear extract, the antibody is used conveniently, and has good effect of cell.
Date published: 2017-10-22
Rated 5 out of 5 by from Nice dark western blot bandWe used this antibody for western blot and got a nice dark band for the correct molecular weight with Jurkat cells
Date published: 2017-01-17
Rated 5 out of 5 by from Produced positive Western blot data of TAL1Produced positive Western blot data of TAL1 expression in non-transfected and human TAL1 transfected 293T whole cell lysates and K-562, Jurkat and CCRF-CEM nuclear extracts. -SCBT QC
Date published: 2014-02-03
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TAL1 抗体 (C-4) is rated 4.2 out of 5 by 5.
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