Date published: 2025-9-8
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TAF II p250 Antibody (6B3) - Western Blotting - Image 1366
TAF II p250 抗体 (6B3): sc-735. HeLa (A) 和 A-431 (B) 全细胞裂解液中 hTAF II p250 表达的 Western 印迹分析.
TAF II p250 抗体 (6B3): sc-735. HeLa (A) 和 A-431 (B) 全细胞裂解液中 hTAF II p250 表达的 Western 印迹分析.

TAF II p250 抗体 (6B3): sc-735

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寫評論提問

说明书
  • TAF II p250 抗体 6B3 是小鼠单克隆 IgG1 κ，TAF II p250抗体, 在69篇文献中引用，规格为200 µg/ml
  • 针对TAF II p250的，从HeLa细胞中分离
  • TAF II p250 抗体 (6B3) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 TAF II p250; 不会与 TAF II p250 of hamster or Drosophila origin产生交联反应
  • 抗TAF II p250抗体（6B3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-735 X, 200 µg/0.1 ml)
  • 关于如何获取TAF II p250 (6B3): sc-735的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 TAF II p250 抗体 (6B3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TAF II p250 抗体 (6B3) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 TAF Basal Transcription Factor 抗体 包括 TAF I p110, TAF I p48, TAF I p95, TAF II p100, TAF II p105, TAF II p130, TAF II p135, TAF II p170, TAF II p18和TAF II p20

TAF II p250 抗体（6B3）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）检测人类 TAF II p250。TAF II p250 单克隆抗体（6B3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。TAF II p250是TFIID复合物的一部分，在基因转录调控中发挥着关键作用，尤其是那些参与细胞周期进展的基因。TFIID复合物对于在TATA启动子处组装预启动复合物至关重要。TAF II p250的结构由多个亚基组成，包括TATA结合蛋白和各种TBP相关因子，它们共同促进基础转录和激活子依赖性转录。TAF II p250已被证实与CCG1相同，后者是一种对细胞周期正常调节至关重要的核DNA结合蛋白。TAF II p250与特定转录因子的相互作用能够精确激活细胞周期进展所需的基因，从而强调了其在细胞功能和发育中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TAF II p250 抗体 (6B3) 参考文献:

  1. 鉴定人类 TFIID 成分以及 250 kDa 多肽与 TATA 盒结合蛋白（TFIID tau）之间的直接相互作用。  |  Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
  2. 与介导转录激活的 TATA 结合蛋白缔合作用的辅助激活子的分离。  |  Dynlacht, BD., et al. 1991. Cell. 66: 563-76. PMID: 1907890
  3. 人类 CCG1 基因对 G1 阶段的进展至关重要，它编码一种 210 千道尔顿的核 DNA 结合蛋白。  |  Sekiguchi, T., et al. 1991. Mol Cell Biol. 11: 3317-25. PMID: 2038334
  4. RNA 聚合酶 II 启动转录过程中的五个中间复合物。  |  Buratowski, S., et al. 1989. Cell. 56: 549-61. PMID: 2917366
  5. 人类 X 染色体基因（CCG1）cDNA 的分子克隆，该基因与 BHK 细胞系的温度敏感 G1 突变体 tsBN462 和 ts13 互补。  |  Sekiguchi, T., et al. 1988. EMBO J. 7: 1683-7. PMID: 3169001
  6. 纯化的 RNA 聚合酶 II 准确启动转录所需的多种因素。  |  Matsui, T., et al. 1980. J Biol Chem. 255: 11992-6. PMID: 7440580
  7. 克隆和表达人类 TAFII250：一种与细胞周期调控有关的 TBP 缔合作用因子。  |  Ruppert, S., et al. 1993. Nature. 362: 175-9. PMID: 7680771
  8. 原生 TATA 盒结合因子 TFIID 的 p250 亚基是细胞周期调控蛋白 CCG1。  |  Hisatake, K., et al. 1993. Nature. 362: 179-81. PMID: 8450888

订购信息

产品名称产品编号规格价格数量收藏夹

TAF II p250 抗体 (6B3)

sc-735
200 µg/ml
RMB2377.00

TAF II p250 (6B3): m-IgG Fc BP-HRP 套装

sc-528118
200 µg Ab; 10 µg BP
RMB2662.00

TAF II p250 (6B3): m-IgGκ BP-HRP 套装

sc-520420
200 µg Ab, 40 µg BP
RMB2662.00

TAF II p250 (6B3): m-IgG1 BP-HRP 套装

sc-542770
200 µg Ab; 20 µg BP
RMB2662.00

TAF II p250 抗体 (6B3) X

sc-735 X
200 µg/0.1 ml
RMB2377.00

TAF II p250 抗体 (6B3) AC

sc-735 AC
500 µg/ml, 25% agarose
RMB3129.00

TAF II p250 抗体 (6B3) HRP

sc-735 HRP
200 µg/ml
RMB2377.00

TAF II p250 抗体 (6B3) FITC

sc-735 FITC
200 µg/ml
RMB2482.00

TAF II p250 抗体 (6B3) PE

sc-735 PE
200 µg/ml
RMB2580.00

TAF II p250 抗体 (6B3) Alexa Fluor® 488

sc-735 AF488
200 µg/ml
RMB2685.00

TAF II p250 抗体 (6B3) Alexa Fluor® 546

sc-735 AF546
200 µg/ml
RMB2685.00

TAF II p250 抗体 (6B3) Alexa Fluor® 594

sc-735 AF594
200 µg/ml
RMB2685.00

TAF II p250 抗体 (6B3) Alexa Fluor® 647

sc-735 AF647
200 µg/ml
RMB2685.00

TAF II p250 抗体 (6B3) Alexa Fluor® 680

sc-735 AF680
200 µg/ml
RMB2685.00

TAF II p250 抗体 (6B3) Alexa Fluor® 790

sc-735 AF790
200 µg/ml
RMB2685.00

To which part of TAF1 protein is this antibody directed? Is it for N or C terminus?

Asked by: Anonymous
Thank you for your question. No epitope mapping has been performed for this particular antibody yet. Its binding site is not known.
Answered by: Tech Support Europe
Date published: 2023-06-30

Can you let me know what was the epitope used to produce this antibody thanks

Asked by: Anonymous
Thank you for your question. TAF II p250 Antibody (6B3): sc-735 was raised against TAF II p250 isolated from HeLa cells. Its epitope region has not been determined yet.
Answered by: Tech Support Europe
Date published: 2019-11-15

Can the TransCruz reagent sc-735 X be used for Western Blot and other applications, or is it exclusively for use in GelShift and ChIP?

Asked by: TinTin
Thank you for your question. The TransCruz form of this antibody, sc-735 X, can be used in applications such as Western Blot or IF/IHC, the recommended dilution factor just needs to be scaled up by 10 due to the increased concentration of the vial.
Answered by: Tech Service
Date published: 2017-01-30
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Rated 3 out of 5 by from OK for WB, but not good for co-IPIt works for Western blot using human cell nuclear extract, but does not work for co-IP at all.
Date published: 2023-04-16
Rated 2 out of 5 by from Faint band after western blotting.It detected a very faint band at the size expected for endogenous TAFII-p250 in lysates prepared from HEK 293T cells (1:500 in 5% milk in TBS-0.1% Tween overnight at 4C).
Date published: 2018-04-09
Rated 1 out of 5 by from TAF1 antibodyI got free sample to test, and it works neither for western nor IP.
Date published: 2017-10-17
Rated 5 out of 5 by from Well characterized TAF II ab for ChIP and WBYour go to monoclonal ab for TAF II for ChIP and WB. With over 45 citations in the primary literature and strong in-house data this is a solid choice.
Date published: 2017-01-07
Rated 5 out of 5 by from Good WB publishable data gathered with HEpGood WB publishable data gathered with HEp-2 cells. -SCBT Publication Review
Date published: 2015-06-24
Rated 5 out of 5 by from no commentno comment
Date published: 2015-05-15
Rated 4 out of 5 by from This antibody worked well for IPThis antibody worked well for IP, published with MCF-7, U2OS and HCT116. -SCBT Publication Review
Date published: 2015-05-04
Rated 5 out of 5 by from Desirable band using HeLa and ADesirable band using HeLa and A-431 whole cell lysates in Western Blot. -SCBT QC
Date published: 2015-05-02
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TAF II p250 抗体 (6B3) is rated 4.3 out of 5 by 14.
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