Date published: 2025-9-7
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T-type Ca<sup>++</sup> CP &alpha;1H Antibody (G-10) - Immunohistochemistry - Image 369551
T 型 Ca++ CP α1H 抗体 (G-10): sc-377510. 对福尔马林固定, 石蜡包埋的人类睾丸组织进行免疫过氧化物酶染色, 显示曲细精管中细胞的微弱胞质染色以及 Leydig 细胞的胞质和细胞核染色.
T 型 Ca++ CP α1H 抗体 (G-10): sc-377510. 对福尔马林固定, 石蜡包埋的人类睾丸组织进行免疫过氧化物酶染色, 显示曲细精管中细胞的微弱胞质染色以及 Leydig 细胞的胞质和细胞核染色.

T-type Ca++ CP α1H 抗体 (G-10): sc-377510

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寫評論提問

说明书
  • T-type Ca++ CP alpha 1H 抗体 G-10 是小鼠单克隆 IgG1 κ，T-type Ca++ CP α1H抗体, 在5篇文献中引用，规格为200 µg/ml
  • 免疫human物种的T-type Ca++ CP α1H的氨基酸2174-2353
  • T-type Ca++ CP alpha 1H 抗体 (G-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 T-type Ca++ CP α1H
  • 抗T-type Ca++ CP alpha 1H抗体（G-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取T-type Ca++ CP α1H (G-10): sc-377510的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 T-type Ca++ CP α1H 抗体 (G-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与T-type Ca++ CP α1H 抗体 (G-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Calcium Channel Protein 抗体包括 L-type Ca++ CP α 1F, L-type Ca++ CP α 1S, L-type Ca++ CP β 1A, L-type Ca++ CP β 2, L-type Ca++ CP β 3, L-type Ca++ CP β 4, L-type Ca++CP γ 2, L-type Ca++CP γ 3, L-type Ca++CP γ 8 和 N-type Ca++ CP α 1B

T型钙离子通道α1H抗体（G-10）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人类来源的T型钙离子通道α1H蛋白。T型钙离子通道蛋白α1H（G-10）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。T型钙离子通道蛋白α1H（也称为CACNA1H）在调节钙离子进入兴奋性细胞方面发挥着至关重要的作用，而钙离子进入兴奋性细胞对于许多生理过程（如肌肉收缩、神经递质释放和基因表达）至关重要。CACNA1H主要位于神经元和肌肉细胞的质膜上，有助于产生低阈值钙电流，而钙电流对于启动动作电位至关重要。T型钙通道具有独特的生物物理特性，可在相对高极化的膜电位下激活，因此这些通道对于调节神经元兴奋性和心脏功能至关重要。T型通道与多种病理生理状况有关，包括癫痫和心律失常，这凸显了它们作为潜在治疗靶点的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

T-type Ca++ CP α1H 抗体 (G-10) 参考文献:

  1. 电压门控 Ca2+ 通道的结构和调节。  |  Catterall, WA. 2000. Annu Rev Cell Dev Biol. 16: 521-55. PMID: 11031246
  2. 与 Ca2+ 通道 Cav1.2 组装在一起的 beta2 肾上腺素能受体信号复合体。  |  Davare, MA., et al. 2001. Science. 293: 98-101. PMID: 11441182
  3. CaV3.2 T型通道介导卵母细胞成熟和受精后的Ca²⁺进入。  |  Bernhardt, ML., et al. 2015. J Cell Sci. 128: 4442-52. PMID: 26483387
  4. 电压门控Ca（2+）通道Cav3.2和乳腺癌的治疗反应。  |  Pera, E., et al. 2016. Cancer Cell Int. 16: 24. PMID: 27034617
  5. 原发性全身性癫痫患儿中的致病性Cav3.2通道突变。  |  Souza, IA., et al. 2019. Mol Brain. 12: 86. PMID: 31651342
  6. Cav3.2 T型钙通道控制小鼠的急性瘙痒。  |  Gadotti, VM., et al. 2020. Mol Brain. 13: 119. PMID: 32873320
  7. 中枢表达的Cav3.2 T型钙通道对神经性疼痛的启动和维持至关重要。  |  Fayad, SL., et al. 2022. Elife. 11: PMID: 36416409
  8. 关节动员的抗痛觉过敏效应需要Cav3.2钙通道。  |  Martins, DF., et al. 2023. Mol Brain. 16: 60. PMID: 37464359
  9. ABT-639对Cav3.2通道活性的影响及其在炎症性和神经性疼痛小鼠模型中的镇痛作用。  |  Antunes, FTT., et al. 2024. Eur J Pharmacol. 967: 176416. PMID: 38342359
  10. Cav3.2缺失可减轻小鼠的非酒精性脂肪肝。  |  Li, X., et al. 2024. Gene. 929: 148812. PMID: 39116959
  11. 钙通道的分子生物学。  |  Perez-Reyes, E. and Schneider, T. 1995. Kidney Int. 48: 1111-24. PMID: 8569073
  12. 电压门控 Ca2+ 通道多样性的分子基础：现在是 T 的时候了吗？  |  Randall, AD. 1998. J Membr Biol. 161: 207-13. PMID: 9493126

订购信息

产品名称产品编号规格价格数量收藏夹

T-type Ca++ CP α1H 抗体 (G-10)

sc-377510
200 µg/ml
RMB2377.00

T-type Ca++ CP α1H (G-10): m-IgG Fc BP-HRP 套装

sc-527606
200 µg Ab; 10 µg BP
RMB2662.00

T-type Ca++ CP α1H (G-10): m-IgG1 BP-HRP 套装

sc-532979
200 µg Ab; 20 µg BP
RMB2662.00

T-type Ca++ CP α1H 抗体 (G-10) AC

sc-377510 AC
500 µg/ml, 25% agarose
RMB3129.00

T-type Ca++ CP α1H 抗体 (G-10) HRP

sc-377510 HRP
200 µg/ml
RMB2377.00

T-type Ca++ CP α1H 抗体 (G-10) FITC

sc-377510 FITC
200 µg/ml
RMB2482.00

T-type Ca++ CP α1H 抗体 (G-10) PE

sc-377510 PE
200 µg/ml
RMB2580.00

T-type Ca++ CP α1H 抗体 (G-10) Alexa Fluor® 488

sc-377510 AF488
200 µg/ml
RMB2685.00

T-type Ca++ CP α1H 抗体 (G-10) Alexa Fluor® 546

sc-377510 AF546
200 µg/ml
RMB2685.00

T-type Ca++ CP α1H 抗体 (G-10) Alexa Fluor® 594

sc-377510 AF594
200 µg/ml
RMB2685.00

T-type Ca++ CP α1H 抗体 (G-10) Alexa Fluor® 647

sc-377510 AF647
200 µg/ml
RMB2685.00

T-type Ca++ CP α1H 抗体 (G-10) Alexa Fluor® 680

sc-377510 AF680
200 µg/ml
RMB2685.00

T-type Ca++ CP α1H 抗体 (G-10) Alexa Fluor® 790

sc-377510 AF790
200 µg/ml
RMB2685.00

Can this antibody use in mouse brain? Does it have the quality of distinguishing the channel from membrane or cytoplasm

Asked by: yumao
Thank you for your question. T-type Ca++ CP &alpha;1H Antibody (G-10): sc-377510 is recommended for detection of T-type Ca++ CP &alpha;1H of human origin by WB, IP, IF, IHC(P) and ELISA. For clear subcellular localization of your target we recommend using a directly fluorescence-conjugated version of this antibody such as sc-377510 AF488.
Answered by: Tech Support Europe
Date published: 2019-07-17

Has this antibody been used in biotynilation assays?

Asked by: Kim02
Thank you for your question. We have not used this antibody in any such application.
Answered by: Technical Support
Date published: 2019-04-26

Could i use this antibody for rat tissue, i bought it according to your recommend (instead of T-type Ca ++ CP alpha 1H Antibody (H-300))?

Asked by: dancer81
Thank you for your question. T-type Ca++ CP &alpha;1H Antibody (G-10): sc-377510 is recommended for detection of T-type Ca++ CP &alpha;1H of human origin by WB, IP, IF, IHC(P) and ELISA. We do not recommend it for detection of the rat epitope. You can find an overview of all Calcium Channel Protein Antibodies in our portfolio by following this link: https://www.scbt.com/scbt/browse/calcium-channel-protein-Antibodies/_/N-zubfk4 For further assistance please contact our technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2025-08-05

We have been using H-300 in our lab. I was wondering if G-10 binds to the same epitope as H-300?

Asked by: Erika28
Thank you for your question. Our monoclonal alternative T-type Ca++ CP &alpha;1H Antibody (G-10): sc-377510 has been raised against the same epitope region as the discontinued polyclonal H-300 clone, amino acids 2174-2353 of T-type Ca++ CP &alpha;1H of human origin.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-11

Can T-type Ca++ CP &alpha;1H (G-10): sc-377510 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, T-type Ca++ CP &alpha;1H (G-10): sc-377510 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from good workThe antibody is used to detect the expression in extracellular matrix tissue, it works very well in WB and we have specific signals.
Date published: 2017-10-31
Rated 1 out of 5 by from Immunohistochemistry frozen & free floatingI tested the antibody for an immunohistochemistry on mouse embryo E12.5, fixed 1h in paraformaldehyde 4%. Dilution: 1/50, Permeabilization solution: PBS 1X- Triton 0,2%(& 0,4%) - Goat serum 10%. It did not work.
Date published: 2017-10-27
Rated 4 out of 5 by from Antibody works well if used as high concentrationsBought this antibody and tested using a number of vectors that contain the C terminus of CACNA1H. Was able to recognize all of them, albeit when used as higher concentrations (1:500). Clean bands though with minimal background.
Date published: 2017-06-13
Rated 5 out of 5 by from Good staing for IHCI got positive result use Hela cell, 1:400 dilution is best, I recomend use it for IHC staining.
Date published: 2017-04-03
Rated 5 out of 5 by from strong bandGood result by WB obtained using human hippocampus tissue from the pre-frontal lobe of the brain,nice strong band.
Date published: 2017-02-05
Rated 5 out of 5 by from Good Western blot data of TGood Western blot data of T-type Ca++ CP 1H expression in human placenta and extracellular matrix tissue extracts. -SCBT QC
Date published: 2013-04-29
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T-type Ca ++ CP α1H 抗体 (G-10) is rated 4.2 out of 5 by 6.
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