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SYP/Synaptophysin 抗体 (H-8): sc-55507

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说明书
  • SYP/Synaptophysin抗体(H-8)是小鼠单克隆IgG1 κ, 在34篇文献中引用,规格为200 µg/ml
  • 免疫human物种的SYP的氨基酸221-313
  • 抗-SYP/Synaptophysin 抗体 (H-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SYP
  • 抗SYP/Synaptophysin抗体(H-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是SYP/Synaptophysin Antibody (H-8) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与SYP/Synaptophysin Antibody (H-8) 打包提供(请参阅下面的订购信息)。
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関連項目

SYP 抗体 (H-8) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过各种应用检测小鼠、大鼠和人样本中的突触素 (SYP),包括免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学和酶联免疫吸附试验 (ELISA)。抗 SYP 抗体 (H-8) 有非共轭型和多种共轭型,如琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物。突触素是一种重要的整体膜蛋白,主要存在于神经元和内分泌细胞中,SYP (H-8) 单克隆抗体在突触小泡的形成和循环中发挥着重要作用。突触素的特点是 N-糖基化和形成六聚体复合物的能力,这有助于发挥大电导通道的功能。SYP 含有四个跨膜结构域,可调节膜胆固醇,这对囊泡的形成至关重要。突触素与突触珠蛋白(VAMP)相互作用,形成一种在神经元发育过程中上调的复合物,但对外渗过程本身并不重要。脑肌球蛋白 V 的尾部结构域与突触珠蛋白 II 和突触素形成稳定的复合物,在去极化过程中,当钙离子流入时,该复合物就会解体,这突显了突触囊泡循环的动态性质以及 SYP 在神经传递中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SYP/Synaptophysin 抗体 (H-8) 参考文献:

  1. Syp Y279、Y304可介导Bcr-Abl与Grb2和其他蛋白的结合。  |  Zhu, K., et al. 1999. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 31: 448-450. PMID: 12115002
  2. 实验室检测新型杀螨剂SYP-9625对红蜘蛛(Boisduval)及其天敌加州捕螨虫(McGregor)的影响。  |  Ouyang, J., et al. 2018. PLoS One. 13: e0199269. PMID: 30395569
  3. 中国果孢菌对DMI杀菌剂SYP-Z048的基线敏感性以及对DMI抗性突变体的分析。  |  Chen, FP., et al. 2012. Plant Dis. 96: 416-422. PMID: 30727143
  4. Syp酪氨酸磷酸酶氨基端SH2结构域的肽复合物的晶体结构。  |  Lee, CH., et al. 1994. Structure. 2: 423-38. PMID: 7521735
  5. 胰岛素受体结合位点与SH2结构域蛋白p85、Syp和GAP的相互作用。  |  Staubs, PA., et al. 1994. J Biol Chem. 269: 27186-92. PMID: 7525547
  6. 胰岛素样生长因子I受体结合位点与SH2结构域蛋白p85、Syp和GTP酶激活蛋白的本地化。  |  Seely, BL., et al. 1995. J Biol Chem. 270: 19151-7. PMID: 7642582
  7. 3T3细胞中催化不活跃的Syp磷酸酶的表达可阻断胰岛素对促分裂原活化蛋白激酶的刺激。  |  Milarski, KL. and Saltiel, AR. 1994. J Biol Chem. 269: 21239-43. PMID: 8063746
  8. 表达Syp水平降低的小鼠的胰岛素信号传导。  |  Arrandale, JM., et al. 1996. J Biol Chem. 271: 21353-8. PMID: 8702915
  9. 大鼠肝癌细胞中一种60千道尔顿的蛋白质在胰岛素受体过度表达的情况下,以胰岛素依赖的方式与酪氨酸磷酸化,并与Syp、磷脂酰肌醇3-激酶和Grb2结合。  |  Zhang-Sun, G., et al. 1996. Endocrinology. 137: 2649-58. PMID: 8770882
  10. 磷脂酶Cγ、酪氨酸磷酸酶Syp以及连接蛋白Shc和Nck对PDGF诱导的DNA合成的作用:证明存在Ras依赖性和非Ras依赖性途径。  |  Roche, S., et al. 1996. EMBO J. 15: 4940-8. PMID: 8890167

订购信息

产品名称产品编号规格价格数量收藏夹

SYP/Synaptophysin 抗体 (H-8)

sc-55507
200 µg/ml
CNY2422.00

SYP/Synaptophysin (H-8): m-IgG Fc BP-HRP 套装

sc-526859
200 µg Ab; 10 µg BP
CNY2715.00

SYP/Synaptophysin (H-8): m-IgG1 BP-HRP 套装

sc-532232
200 µg Ab; 20 µg BP
CNY2715.00

SYP/Synaptophysin 抗体 (H-8) AC

sc-55507 AC
500 µg/ml, 25% agarose
CNY3189.00

SYP/Synaptophysin 抗体 (H-8) HRP

sc-55507 HRP
200 µg/ml
CNY2422.00

SYP/Synaptophysin 抗体 (H-8) FITC

sc-55507 FITC
200 µg/ml
CNY2527.00

SYP/Synaptophysin 抗体 (H-8) PE

sc-55507 PE
200 µg/ml
CNY2625.00

SYP/Synaptophysin 抗体 (H-8) Alexa Fluor® 488

sc-55507 AF488
200 µg/ml
CNY2738.00

SYP/Synaptophysin 抗体 (H-8) Alexa Fluor® 546

sc-55507 AF546
200 µg/ml
CNY2738.00

SYP/Synaptophysin 抗体 (H-8) Alexa Fluor® 594

sc-55507 AF594
200 µg/ml
CNY2738.00

SYP/Synaptophysin 抗体 (H-8) Alexa Fluor® 647

sc-55507 AF647
200 µg/ml
CNY2738.00

SYP/Synaptophysin 抗体 (H-8) Alexa Fluor® 680

sc-55507 AF680
200 µg/ml
CNY2738.00

SYP/Synaptophysin 抗体 (H-8) Alexa Fluor® 790

sc-55507 AF790
200 µg/ml
CNY2738.00

I am using SYP (H-8): sc-55507 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Histologistics from Works in Mouse and HumanWe got this as a sample. Performed on FFPE human and mouse tissues. Sodium citrate 30 minutes at 1:250. Very easy and looks great!
Date published: 2017-11-17
Rated 4 out of 5 by Tsen from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by DefinitelyNotMatt from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-25
Rated 4 out of 5 by Valencia from Great antibodyGreat antibody. It worked well without any problems.
Date published: 2015-10-07
Rated 4 out of 5 by CJ from Various publishable data in WB using mouseVarious publishable data in WB using mouse cerebral cortex and hippocampal tissues -SCBT Publication Review
Date published: 2015-06-30
Rated 4 out of 5 by HF from Publishable data in WB using rat brainPublishable data in WB using rat brain. -SCBT Publication Review
Date published: 2015-06-06
Rated 4 out of 5 by CJ from Publishable data in IHC using human DAOYPublishable data in IHC using human DAOY,G401,central nervous system atypical teratoid rhabdoid tumor (AT/RT) -SCBT Publication Review
Date published: 2015-04-21
Rated 3 out of 5 by ET from Robust bands detected in WB using IMRRobust bands detected in WB using IMR-32 whole cell lysate and rat and mouse brain tissue extracts. -SCBT QC
Date published: 2015-04-17
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SYP/Synaptophysin 抗体 (H-8) is rated 4.2 out of 5 by 10.
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