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SYND4/SDC4/Syndecan-4 Antibody (5G9) - Western Blotting - Image 352774
Syndecan-4 抗体 (5G9): sc-12766. HeLa (A) 和 MDA-MB-231 (B) 全细胞裂解液中 Syndecan-4 表达的 Western 印迹分析.
Syndecan-4 抗体 (5G9): sc-12766. HeLa (A) 和 MDA-MB-231 (B) 全细胞裂解液中 Syndecan-4 表达的 Western 印迹分析.

SYND4/SDC4/Syndecan-4 抗体 (5G9): sc-12766

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说明书
  • SYND4/SDC4/Syndecan-4 抗体 5G9 是小鼠单克隆 IgG2a κ，SYND4/SDC4/Syndecan-4抗体, 在99篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Syndecan-4
  • SYND4/SDC4/Syndecan-4 抗体 (5G9) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 the ectodomain of Syndecan-4
  • 抗SYND4/SDC4/Syndecan-4抗体（5G9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SYND4/SDC4/Syndecan-4 (5G9): sc-12766的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是SYND4/SDC4/Syndecan-4 Antibody (5G9) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与SYND4/SDC4/Syndecan-4 Antibody (5G9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Syndecan抗体，包括Syndecan-1, Syndecan-2, Syndecan-3和Syndecan-4

Syndecan-4抗体（5G9）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测小鼠、大鼠和人类中的Syndecan-4，也称为amphiglycan或ryudocan。抗Syndecan-4抗体（5G9）针对人类Syndecan-4抗原表位，可用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）。Syndecan-4是一种I型整合膜蛋白聚糖，在细胞-细胞外基质粘附中起着关键作用，与整合素协同促进细胞扩散、焦点粘附组装和肌动蛋白应力纤维的形成。这些功能对于细胞迁移、组织修复和信号转导等过程至关重要，凸显了Syndecan-4在维持细胞结构和通信中的重要性。Syndecan-4 单克隆抗体（5G9）有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 变体，可满足各种实验需求。通过靶向 Syndecan-4，研究人员可以探索其在关键生理和病理机制中的参与，包括癌症转移、伤口愈合和发育生物学。抗 Syndecan-4 抗体 (5G9) 是研究 Syndecan-4 在不同生物学环境中的复杂相互作用和功能的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SYND4/SDC4/Syndecan-4 抗体 (5G9) 参考文献:

  1. 在局灶黏合剂和肌动蛋白应力纤维的组装过程中，Syndecan-4 以 Rho 依赖性方式与整合素协同发出信号。  |  Saoncella, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 2805-10. PMID: 10077592
  2. B 淋巴细胞（MPC-11）与 I 型胶原蛋白的黏合剂是由整体膜蛋白多糖--辛迪加介导的。  |  Sanderson, RD., et al. 1992. J Immunol. 148: 3902-11. PMID: 1602136
  3. 细胞表面蛋白多糖 Syndecan 家族：细胞外效应分子的发育调节受体。  |  Salmivirta, M. and Jalkanen, M. 1995. Experientia. 51: 863-72. PMID: 7556568
  4. 小鼠胚胎发育过程中纤维聚糖（syndecan-2）表达的时空变化  |  David, G., et al. 1993. Development. 119: 841-54. PMID: 8187643
  5. 中枢神经系统胚胎发育过程中辛迪加-1 和辛迪加-3 的表达与 midkine 结合的关系。  |  Nakanishi, T., et al. 1997. J Biochem. 121: 197-205. PMID: 9089390

订购信息

产品名称产品编号规格价格数量收藏夹

SYND4/SDC4/Syndecan-4 抗体 (5G9)

sc-12766
200 µg/ml
RMB2377.00

SYND4/SDC4/Syndecan-4 (5G9): m-IgG2a BP-HRP 套装

sc-545913
200 µg Ab; 10 µg BP
RMB2662.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) AC

sc-12766 AC
500 µg/ml, 25% agarose
RMB3129.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) HRP

sc-12766 HRP
200 µg/ml
RMB2377.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) FITC

sc-12766 FITC
200 µg/ml
RMB2482.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) PE

sc-12766 PE
200 µg/ml
RMB2580.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) Alexa Fluor® 488

sc-12766 AF488
200 µg/ml
RMB2685.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) Alexa Fluor® 546

sc-12766 AF546
200 µg/ml
RMB2685.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) Alexa Fluor® 594

sc-12766 AF594
200 µg/ml
RMB2685.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) Alexa Fluor® 647

sc-12766 AF647
200 µg/ml
RMB2685.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) Alexa Fluor® 680

sc-12766 AF680
200 µg/ml
RMB2685.00

SYND4/SDC4/Syndecan-4 抗体 (5G9) Alexa Fluor® 790

sc-12766 AF790
200 µg/ml
RMB2685.00

Do you know if this can be used as blocking antibody?

Asked by: Davide90
Thank you for your question. Unfortunately this antibody has not been tested for blocking applications so we do not have information regarding this use.
Answered by: Tech Service
Date published: 2019-07-08

I would like to know if this antibody sc-12766 could detect mouse shed syndecan-4 (ectodomain) in serum by WB? If yes, any publications or data to support it?

Asked by: wuchen
Thank you for your question. Here is a publication that states that this antibody detects the ectodomain of Syndecan-4: PMID: 9565572. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-21

What is the best secondary to use with Syndecan-4 Antibody (5G9): sc-12766  in Western Blot?

Asked by: cjMara
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 5 out of 5 by from Works well on westernAntibody works well to detect transfected Syndecan-4 by western.
Date published: 2022-09-27
Rated 4 out of 5 by from lane is very clearthe product we bought was SDC4, we got an excellent result in COIP
Date published: 2022-05-15
Rated 2 out of 5 by from Molecular weightI would like to know what size I should see the band by western. In theory at 24kda, but it doesn't..
Date published: 2018-09-21
Rated 5 out of 5 by from Worked well in mouse tissue for IHC-PStain worked well for IHC-P in mouse tissue. I did a 1:50 dilution.
Date published: 2017-09-22
Rated 5 out of 5 by from Works wellQC controled antibody, this antibody detects target protein at 1:800 dilution by WB . Works well detect target protein in mouse kidney tissue,very great.
Date published: 2017-09-02
Rated 5 out of 5 by from Positive signalPositive signal observed in Western blot. Recommended for detection of mouse Syndecan-4.
Date published: 2017-05-27
Rated 5 out of 5 by from specificity is goodGood specificity and signal strength in western blot analysis of Syndecan-4 expression in Hela cell.
Date published: 2017-02-12
Rated 5 out of 5 by from Best choice for Syndecan-4If you need to detect syndecan-4, look no further. This antibody will get you the results you need in mouse, rat, or human. It is a proven winner with over 70 publications to support it.
Date published: 2017-01-11
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SYND4/SDC4/Syndecan-4 抗体 (5G9) is rated 4.4 out of 5 by 15.
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